Pseudorabies virus for tumor treatment

A pseudorabies virus, tumor technology, applied in the field of virus and tumor treatment

Active Publication Date: 2019-08-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, pseudorabies virus is widely used as a veterinary vaccine, etc., and there is no report that any pseudorabies virus has oncolytic activity in this field

Method used

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  • Pseudorabies virus for tumor treatment
  • Pseudorabies virus for tumor treatment
  • Pseudorabies virus for tumor treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0147] Example 1: Acquisition and preparation of PRV and its modified form

[0148] 1.1 Isolation of pseudorabies virus PRV from specimens

[0149] (1) The throat swabs and anal swabs of sick pigs are from China, Xiamen City Center for Disease Control and Prevention, pig embryo kidney cells (PK-15; ATCC NumberCCL-33) TM ) It is preserved by Xiamen University, China, National Infectious Disease Diagnostic Reagents and Vaccine Engineering Technology Research Center, and cultured in DMEM medium supplemented with 10% fetal bovine serum, glutamine, penicillin and streptomycin.

[0150] (2) Specimen processing: Fully stir the throat swab and anal swab in the specimen storage solution to wash off the virus and virus-containing cells on the swab, and then put the specimen storage solution at 4000 rpm at 4°C Centrifuge at high speed for 30 minutes;

[0151] (3) Vaccination and observation:

[0152] A. Spread PK-15 cells on a 24-well plate, the number of cells in each well is 1×10 5 Aspirate t...

Embodiment 2

[0165] Example 2: Evaluation of in vitro anti-tumor activity of wild-type PRV

[0166] 2.1 Virus and cell line used

[0167] (1) Virus: This example uses the PRV-WT (SEQ ID NO:1) provided in Example 1.

[0168] (2) Cell line: human rhabdomyosarcoma cell RD( Number:CCL-136 TM ); Human colorectal cancer cell line SW1116( Number:CCL-233 TM ), SW480( Number:CCL-228 TM ) And HT-29 ( Number:HTB-38 TM ); Human gastric cancer cell line AGS ( Number:CRL-1739 TM ), SGC7901 (CCTCC deposit number: GDC150), BGC823 (CCTCC deposit number: GDC151) and NCI-N87 ( Number:CRL-5822 TM ); human esophageal cancer cell line TE-1 (purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, number: 3131C0001000700089); human small cell lung cancer cell line DMS114 ( Number:CRL-2066 TM ); Human non-small cell lung cancer cell line SPC-A-1 (CCTCC deposit number: GDC050), NCI-H1975 ( Number:CRL-5908 TM ), NCI-H1299( Number:CRL-5803 TM ), A5...

Embodiment 3

[0185] Example 3: In vivo anti-tumor experiment of wild-type PRV

[0186] 3.1 Viruses, cell lines and laboratory animals

[0187] (1) Virus: The PRV-WT provided in Example 1 is used in this example. Refer to Examples 2.2 and 2.3 for virus culture and virus titer determination methods, respectively.

[0188] (2) Cell line: human nasopharyngeal carcinoma cell line CNE1, human Burkitt's lymphoma cell Raji ( Number:CCL-86 TM ), human glioma cell line GBM (primary tumor cell line isolated from patient tumor tissue), mouse colon cancer cell CT26, mouse liver cancer cell Hep1-6, mouse kidney cancer cell Renca and mouse breast cancer cell 4T1 . The above cells except Raji use RPMI-1640 medium, all other cells are cultured in DMEM medium, and the above medium is supplemented with 10% fetal bovine serum, glutamine and penicillin-streptomycin double antibody. All the above cells are at 37℃, 5% CO 2 Culture under standard conditions.

[0189] (3) Experimental animals: Female C.B17SCID mice ...

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Abstract

The invention relates to the field of viruses and the field of tumor treatment, in particular to a pseudorabies virus (PRV) or a modification form thereof, or a genome sequence or cDNA sequence with the PRV and the modification form of the PRV, or a nucleic acid molecule with a compensatory sequence of the cDNA sequence, application of the nucleic acid molecule to treating tumors and / or reducing or restraining tumor relapse in a testee (such as human), and application to preparing a pharmaceutical composition for treating the tumors and / or reducing or restraining the tumor relapse in the testee (such as human). The invention further relates to a method for treating the tumors and / or reducing or restraining the tumor relapse. The method includes the step of applying the PRV or the modification form of the PRV to the testee needing the PRV or the modification form of the PRV or the nucleic acid molecule with the genome sequence of the PRV or the modification form of the PRV.

Description

Technical field [0001] The present invention relates to the field of viruses and tumor treatment. Specifically, the present invention relates to pseudorabies virus (PRV) or its modified form, or a nucleic acid molecule comprising the genome sequence of PRV or its modified form, for use in the treatment of tumors and / or reduction in a subject (e.g., human) Or the use of inhibiting tumor recurrence, and the use of preparing drugs for treating tumors and / or reducing or inhibiting tumor recurrence in subjects (for example, humans). The present invention also relates to a method for treating tumors and / or reducing or inhibiting tumor recurrence, which comprises administering PRV or a modified form thereof, or a nucleic acid molecule comprising a genomic sequence of PRV or a modified form thereof to a subject in need thereof step. Background technique [0002] The current treatment methods for malignant tumors mainly include surgery, chemotherapy and radiotherapy. These traditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/763A61K45/06A61P35/00C12N15/38C12N7/00
CPCA61K35/763A61K45/06A61P35/00C07K14/005C12N7/00C12N2710/16721C12N2710/16722C12N2710/16732A61K2300/00
Inventor 陈毅歆王国松付饶沈晨光林丽娜夏宁邵
Owner XIAMEN UNIV
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