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Method for extracting polysaccharide and d-mannitol from mogroside extraction waste liquid

A technology of mogroside and mannitol, which is applied in the field of extracting polysaccharides and D-mannitol, can solve the problems of not being able to extract polysaccharides from grosvenoria at the same time, require high production equipment conditions, and use a large amount of solvent, etc., to achieve continuous large-scale production, The effect of low cost and short extraction time

Active Publication Date: 2021-06-25
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses flammable and explosive ethyl acetate, which not only consumes a large amount of solvent, but also is difficult to recycle, has high requirements on production equipment conditions, high cost, and cannot extract polysaccharides from Luo Han Guo at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[0061] Before the anion exchange resin of the embodiment of the present invention is used, it is soaked in 2BV ethanol solution with a volume fraction of 95% for 24 hours, and then washed with an ethanol solution with a volume fraction of 95% until the effluent is colorless and odorless, and washed with water until it has no alcohol smell, and then Alkaline wash with 4BV of 5% sodium hydroxide solution, then wash with water until neutral, then pickle with 4BV of 5% hydrochloric acid solution, then wash with water until neutral, and finally oxidize with 4BV of 5% hydrogen Sodium solution alkaline washing, and then washing to neutral, Serve.

reference example 2

[0063] Before using the gel resin of the embodiment of the present invention, it is swollen with boiling water for 3 hours, then evenly filled into the resin column by wet method, the air bubbles are removed, and it is eluted with 8BV of water.

Embodiment 1

[0065] (1) Ultrafiltration and decolorization: Boil 24t mogroside extraction waste liquid to 95°C, keep it warm for 30min, sterilize it with UHT at 120°C and 0.5MPa for 15s, cool it down to room temperature in plate type, and put 23.5t sterilized liquid Use a ceramic membrane with a pore size of 0.3 μm for ultrafiltration, add water to wash the filter residue on the ceramic membrane until the permeate is colorless and transparent with a sugar content of 0, 28.5 tons of permeate (solid content: 2.0%) at a flow rate of 0.5BV / h, Put on the D-941 anion exchange resin column (the diameter-to-height ratio of the column is 1:4, the volume of the D-941 anion exchange resin is 4000L), collect the effluent, and then wash with water at a flow rate of 0.5BV / h until the sugar content is 0 , collect the washing solution, and concentrate under reduced pressure at 70°C and -0.08MPa to a solid content of 20%, to obtain 2.35t of decolorized concentrated solution;

[0066] (2) Gel column chromat...

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Abstract

The method for extracting polysaccharides and D-mannitol from the mogroside extraction waste liquid comprises the following steps: (1) boiling the mogroside extraction waste liquid, keeping warm, sterilizing, cooling, ultrafiltration, washing with water, passing through Put the anion exchange resin column on the liquid, collect the effluent, wash with water, collect the washing liquid, and concentrate; (2) put on the gel resin column, elute isocraticly, collect the target eluate in sections, and concentrate; Add alcohol solution to the concentrated solution, stir, stand still, filter, and dry to obtain the Luo Han Guo polysaccharide product; concentrate the filtrate and mix it with the crude D-mannitol concentrate, crystallize, filter, wash with alcohol, and dry to obtain the D-mannitol product . The mass content of Luo Han Guo polysaccharide obtained by the method of the present invention is more than or equal to 95%, and the yield is more than or equal to 92%; the mass content of the obtained D-mannitol is more than or equal to 98%, and the yield is more than or equal to 92%, and the quality is stable; Low, less waste liquid discharge, large-scale production can be realized.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides and D-mannitol, in particular to a method for extracting polysaccharides and D-mannitol from mogroside extraction waste liquid. Background technique [0002] Luo Han Guo is a perennial vine of Cucurbitaceae Luo Han Guo, mainly produced in Guilin, Guangxi. The fruit of Luo Han Guo is of the same origin as medicine and food, and has high nutritional value and medicinal value. The nutritional ingredients mainly include mogrosides, sugars, proteins, flavonoids, amino acids, vitamins and mineral elements. It has the effects of clearing away heat and detoxification, moistening the lungs and relieving asthma, promoting body fluid and relieving cough, moistening the intestines and detoxifying. [0003] The main components of mogroside sweeteners are mogroside Ⅲ, mogroside Ⅳ, mogroside Ⅴ, mogroside ⅡE, mogroside ⅢE, etc. Among them, the content and sweetness of mogroside Ⅴ in Luo Han Guo are rela...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07C29/74C07C31/26
CPCC07B2200/07C07C29/74C08B37/0003C07C31/26
Inventor 陈钱黄华学贺进军
Owner HUNAN HUACHENG BIOTECH
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