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Method of determining trace mercury ions in Eleocharis tuberose peel based on DNase SERS technology

A DNA enzyme and water chestnut skin technology, applied in the field of analysis and detection, can solve the problems of inconvenient operation of mercury ions, low sensitivity and the like

Inactive Publication Date: 2019-08-09
HEZHOU UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing surface-enhanced Raman scattering spectrometry method for the determination of mercury ions has disadvantages such as inconvenient operation and low sensitivity.

Method used

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  • Method of determining trace mercury ions in Eleocharis tuberose peel based on DNase SERS technology
  • Method of determining trace mercury ions in Eleocharis tuberose peel based on DNase SERS technology
  • Method of determining trace mercury ions in Eleocharis tuberose peel based on DNase SERS technology

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Embodiment Construction

[0037] The present invention will be described in further detail below in conjunction with specific examples, which do not constitute any limitation to the present invention.

[0038] Experimental reagents:

[0039] 2.4×10 -2 mol / L AgNO 3 (analytical pure, Guangdong Guanghua Science and Technology Co., Ltd.); 0.5g / L NaBH 4 (analytical pure, Jin Guangfu Fine Chemical Research Institute); 2mol / LNaCl (analytical pure, Xilong Chemical Co., Ltd.); 0.1mol / LHCl (36~38%, Shantou Xilong Chemical Factory Co., Ltd.); 10g / L Trisodium citrate (dihydrate) (Xilong Chemical Factory, Shantou, Guangdong); 5.23×10 -5 mol / L rhodamine 6G (Rh6G) (MERCK company of the United States); 0.2mol / L trishydroxymethylaminomethane (Tris) (Chengdu Kelong Chemical Reagent Factory); 0.2mol / L edetate disodium ( EDTANa 2 ) (Chengdu Kelong Chemical Reagent Factory); mixed solution: add 3.72mL 0.2mol / L EDTANa successively 2 , 5mL 2mol / L NaCl, 0.5mL0.2mol / L Tris, mix well and dilute to 16mL, add EDTANa 2 Calc...

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Abstract

The invention discloses a method of determining trace mercury ions in Eleocharis tuberose peel based on a DNase SERS technology. The method comprises the following steps: a Hg<2+> standard solution system with a known concentration and a blank reference solution system are prepared, SERS peak intensities of the Hg<2+> standard solution system with a known concentration and the blank reference solution system are determined respectively, deltaI is calculated, a working curve is drawn for the concentration relationship of Hg<2+> by deltaI, and based on the above method, the content of Hg<2+> inthe Eleocharis tuberose peel is further determined. The SERS spectrometry result shows that the linear regression equation of the Hg<2+> concentration and deltaI is deltaI=0.8685x+17.92, R<2>=0.9955,and a good linear relationship with deltaI is achieved when the Hg<2+> concentration is between 2.5*10<-8> and 7.5*10<-7>. When the method is used for Hg<2+> detection for the Eleocharis tuberose peel, the determined relative standard deviations are all within 10%, and the recovery rate is 101 to 105%. The method has the characteristics of simple operation and high sensitivity and can be used fordetecting heavy metals in agricultural products.

Description

[0001] 【Technical field】 [0002] The invention belongs to the field of analysis and detection, and in particular relates to a method for detecting trace mercury ions in water chestnut skins based on the DNase SERS technology with simple operation and high sensitivity. [0003] 【Background technique】 [0004] Mercury is a highly toxic heavy metal. Mercury and its compounds can easily accumulate in the human body through the food chain. When the mercury content accumulates in the human body to a certain extent, it will cause poisoning. For a long time, due to unreasonable discharge of industrial wastewater, improper treatment of domestic waste, and large-scale use of chemical fertilizers and chemical pesticides, a large amount of heavy metal mercury has remained in agricultural products. Human beings eat agricultural products contaminated by mercury for a long time, and mercury accumulates in the human body, leading to chronic or acute mercury poisoning in the human body. The p...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28G01N1/44
CPCG01N1/28G01N1/44G01N21/658
Inventor 黎小椿庞永丰罗杨合聂辉伍淑婕段振华
Owner HEZHOU UNIV
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