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A kind of preparation method of peony peptide selenium chelate

A technology of chelate and peony peptide, which is applied in the field of preparation of peony peptide selenium chelate, can solve the problems of low production purity and low selenium chelation amount, and achieve the effect of easy precipitation and increased yield

Inactive Publication Date: 2021-03-23
世堃堂(广东)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current protein peptide selenium chelates generally have low production purity and low selenium chelation capacity.

Method used

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  • A kind of preparation method of peony peptide selenium chelate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of peony peptide selenium chelate, comprising the following steps:

[0024] Preparation of S1 peony seed protein peptide;

[0025] S2 configures the prepared peony seed protein peptide into a solution with a mass fraction of 9% with deionized water;

[0026] Add 0.8mmol / L selenite to S3 and mix evenly, adjust the pH of the solution to 5, and stir at 55°C for 50min;

[0027] S4 Add a mixed solution of ethanol and tert-butanol twice the volume of the mixed solution, the volume ratio of ethanol and tert-butanol is 1:3, let it stand for 24 hours, keep the precipitate, and dry it in vacuum at 35°C.

[0028] The preparation of the above step S1 peony seed protein peptide comprises the following steps:

[0029] S11 extracting peony protein from peony seed meal;

[0030] S12 extracts peony protein with supercritical carbon dioxide;

[0031] S13 Take peony protein, add distilled water to dissolve the peony protein according to the mass ratio of 1:20, adj...

Embodiment 2

[0037] A preparation method of peony peptide selenium chelate, comprising the following steps:

[0038] Preparation of S1 peony seed protein peptide;

[0039] S2 configures the prepared peony seed protein peptide into a solution with a mass fraction of 14% with deionized water;

[0040] Add 1.2mmol / L selenite to S3 and mix evenly, adjust the pH of the solution to 5.5, and stir at 55°C for 90min;

[0041] S4 Add a mixed solution of ethanol and tert-butanol which is 3 times the volume of the mixed solution, the volume ratio of ethanol and tert-butanol is 1:5, let it stand for 36 hours, keep the precipitate, and dry it under vacuum at 40°C.

[0042] The preparation of the above step S1 peony seed protein peptide comprises the following steps:

[0043] S11 extracting peony protein from peony seed meal;

[0044] S12 extracts peony protein with supercritical carbon dioxide;

[0045] S13 Take peony protein, add distilled water to dissolve the peony protein according to the mass r...

Embodiment 3

[0051] A preparation method of peony peptide selenium chelate, comprising the following steps:

[0052] Preparation of S1 peony seed protein peptide;

[0053] S2 configures the prepared peony seed protein peptide into a solution with a mass fraction of 10% with deionized water;

[0054] Add 0.9mmol / L selenite to S3 and mix evenly, adjust the pH of the solution to 5.2, and stir at 55°C for 60min;

[0055] S4 Add a mixed solution of ethanol and tert-butanol twice the volume of the mixed solution, the volume ratio of ethanol and tert-butanol is 1:4, let it stand for 24 hours, keep the precipitate, and dry it in vacuum at 37°C.

[0056] The preparation of the above step S1 peony seed protein peptide comprises the following steps:

[0057] S11 extracting peony protein from peony seed meal;

[0058] S12 extracts peony protein with supercritical carbon dioxide;

[0059] S13 Take peony protein, add distilled water to dissolve the peony protein according to the mass ratio of 1:20, ...

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Abstract

The invention relates to the field of plant extracts, in particular to a preparing method of a peony peptide selenium chelate. The method comprises the following steps of S1, preparing peony seed protein peptides; S2, preparing the prepared peony seed protein peptides into a solution with the mass fraction of 9-14% through deionized water; S3, adding 0.8-1.2 mmol / L selenite, uniformly mixing the selenite and the solution, adjusting the pH of the mixed solution to be 5-5.5, and conducting stirring for 50-90 minutes under the condition of 55-60 DEG C; S4, adding a mixed solution of ethyl alcoholand tertiary butanol which accounts for 2-3 times the volume of the mixed solution, conducting standing for 24-36 hours, preserving a precipitate, and conducting vacuum drying on the precipitate at 35-40 DEG C, wherein the volume ratio of the ethyl alcohol to the tertiary butanol is 1:(3-5). According to the preparing method, the peony peptides and inorganic selenium are adopted for chelation, and compared with chelation of other protein peptides with selenium, the chelation rate of the peony peptides is higher. The reasonable pH is adopted, and the yield of the peony peptide selenium chelateis increased. The ethyl alcohol and the tertiary butanol are adopted for alcohol precipitation, so that the peony peptide selenium chelate is more easily precipitated.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to a preparation method of peony peptide selenium chelate. [0002] technical background [0003] Peony is a plant of Ranunculaceae and Paeoniae genus, known as "National Beauty and Heavenly Fragrance". Peony seed oil developed from peony seeds is a natural pollution-free food integrating nutrition and health care. The protein content of peony seed meal, one of the by-products of pressing peony seed oil, reaches 19.03%. [0004] Protein is an important component of food. Protein peptides are obtained after enzymatic hydrolysis. Its functional and biological characteristics are quite different from the original protein. For example, the solubility, emulsification and foaming of protein can be improved after enzymatic hydrolysis. In my country, there are a large number of peony seed meal products after oil production every year, and a large amount of peony protein can be obtained. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/107C07K1/30C12P21/06
CPCC07K1/1077C07K1/30C12P21/06Y02P20/54
Inventor 谢瑞生王世英赵欣
Owner 世堃堂(广东)生物科技有限公司