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Pediococcus pentosaceus and application thereof

A kind of Pediococcus pentosaceus, spherical technology, applied in the application, bacteria, microorganisms and other directions, can solve the problem of low product activity, loss of activity and other problems

Active Publication Date: 2019-08-16
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This effect causes a huge change in the molecular structure of mycotoxins. This process is irreversible in animals and humans, and the converted products have very low or no activity.

Method used

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  • Pediococcus pentosaceus and application thereof
  • Pediococcus pentosaceus and application thereof
  • Pediococcus pentosaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Screening, Identification and Detoxification Mechanism of Efficient Detoxification ZEN Probiotic Strain

[0032] 1.1 Source of bacteria

[0033] 15 strains of lactic acid bacteria preserved in the clinical laboratory of the College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, as follows: Streptococcus lactis, Lactobacillus rhamnosus, Pediococcus thermophiles, Lactobacillus acidophilus, Lactobacillus brevis, Enterococcus lactis, Bacillus subtilis, Lactobacillus casei.

[0034] 1.2 Main medium preparation

[0035] Lactic acid bacteria medium (MRS): peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, sulfuric acid Manganese 0.25g, solid medium plus agar powder 18g, distilled water 1000mL, pH 6.2~6.6

[0036] MacConkey medium (MAC): peptone 20g, sodium chloride 5g, lactose 10g, agar 20g, distilled water 1...

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Abstract

The invention discloses Pediococcus pentosaceus. Identification shows that the Pediococcus pentosaceus is a quadruple spherical Gram-positive facultative anaerobe. The preservaition number of the Pediococcus pentosaceus is CCTCC No. M2018363. The detoxification rate of the Pediococcus pentosaceus xy46 to zearalenone (ZEN) viable bacteria with the concentration being 4 microgram / mL is 60.4%, and the detoxification rate reaches up to 94.4% under a deactivation condition. The Pediococcus pentosaceus has an evident protecting effect on mouse reproductive system, reproductive organ and reproductivecell damage caused by zearalenone (ZEN) with the concentration being 40mg / kg and 70mg / kg. Therefore, the Pediococcus pentosaceus can be used as a feed additive to prevent the toxic influence of the zearalenone on animals.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a strain of Pediococcus pentosaceae and its application. Background technique [0002] Current studies suggest that the main toxic mechanism of zearalenone (ZEN) is to interfere with the estrogen signaling pathway, thereby affecting the overall function of the reproductive system. There are mainly two interference pathways: the chemical structure of ZEN is very similar to 17β-estradiol (17β-E2), which can competitively bind to estrogen receptors, thereby interfering with estrogen; in addition, ZEN and steroids It also has a similar structure, which can compete with it for the binding sites of 3α-hydroxysteroid dehydrogenase (3α-HSD) and 3β-hydroxysteroid dehydrogenase (3β-HSD), affect the synthesis of reproductive hormones, and interfere with the estrogen signaling pathway effect, leading to disturbances in the function of the reproductive system. [0003] At present, the elimi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/01
CPCA23K10/18C12R2001/01C12N1/205
Inventor 龙淼潘佳雯何剑斌李鹏杨淑华张燚王楠王明阳
Owner SHENYANG AGRI UNIV