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Novel biosensor for heavy metal mercury contaminant detection

A biosensor, heavy metal technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as restricting applications, and achieve the effects of facilitating commercialization, improving sensitivity, and increasing solid load

Inactive Publication Date: 2019-08-20
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, tedious sample preparation and expensive equipment limit the application of these instrumental methods

Method used

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  • Novel biosensor for heavy metal mercury contaminant detection
  • Novel biosensor for heavy metal mercury contaminant detection
  • Novel biosensor for heavy metal mercury contaminant detection

Examples

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Embodiment 1

[0044] Step 1. Add 2g Cu(NO 3 ) 2 ·H 2 O dissolved in 80mL C 2 h 6 O middle. Then, add 60mL NH 4 OH (25%) and 20mL NaOH (1mol L -1 ). After stirring the resulting mixed solution for 20 minutes, a clear solution was formed, which was sealed and heated on a hot plate at 130° C. for 12 hours, then cooled to room temperature. It was centrifuged and washed three times with ultrapure water. Finally, it was dried on a hot plate at 90°C for 8 hours to obtain dried CuO powder;

[0045] Step 2. Synthesize Au NPs first. Dissolve 0.01 g of Pluronic F127 in 1 mL of HAuCl under constant stirring 4 4H 2 O middle. Then, 1mL AA (0.4mol L -1 ) was added to the above solution. After the mixture was stirred at 600 rpm for 3 hours, the product was centrifuged at 12000 rpm for 20 minutes and washed three times with ultrapure water. Disperse the Au NPs in 1 mL of ultrapure water. The synthesis procedure of D-CuO / Au is as follows, 2mg D-CuO was added into 1mL ultrapure water and sonic...

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Abstract

The invention relates to a preparation method of a biosensor for heavy metal mercury contaminant detection, and an application thereof, and belongs to the technical field of the electrochemical detection. The novel biosensor is characterized by using TB as a trace indicator to perform redox reaction so as to provide an electric signal; and performing signal amplification by utilizing D-CuO / Au composite material and an HCR strategy, thereby further improving the sensitivity of the manufactured biosensor. The strategy utilizes a long-incision and duplex DNA structure; a large amount of TB molecules can be adhered to the double-stranded DNA through the pai-pai stacking on the DNA basic group, thereby realizing the electrochemical response of the amplification of the TB molecules. Furthermore,the specificity of the proposed biosensor is guaranteed through the T-Hg2+-T basic group, and can distinguish the Hg2+ and other interference metal ion. The sensor is successfully used for detectingthe mercury contaminant in the environment. The biosensor disclosed by the invention has the advantages of being high in sensitivity, strong in specificity and convenient to detect. The novel biosensor disclosed by the invention provides experiment evidence for the research and development of the mercury contaminant detection technology, thereby proving a new thought and new technology platform for the mercury contaminant monitoring in the environment.

Description

[0001] Technical field: [0002] The invention relates to a preparation method and application of a biosensor for quantitatively detecting heavy metal mercury ions in the environment, especially layered porous dandelion-shaped copper oxide microspheres based on gold nano-modification as the matrix material of the sensing platform, combined with toluidine blue A biosensor with hybridization chain reaction as a signal amplification strategy, and the use of thymine-thymine (T-T) base mismatch can specifically capture mercury ions to form T-Hg 2+ The -T base pair mechanism is used to detect heavy metal mercury ions, and belongs to the field of electrochemical detection. [0003] Background technique: [0004] Mercury ion (Hg 2+ ) is one of the most dangerous heavy metal pollutants naturally occurring in the environment. Mercury pollution is common in different ecosystems such as atmosphere, soil and water. In addition, exposure to even low concentrations of mercury can cause a r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 何俊琳于超余雨洁
Owner CHONGQING MEDICAL UNIVERSITY
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