Application of saikosaponin B2 in preparation of antidepressant drug

A saikosaponin and antidepressant technology, applied in the field of biomedicine

Inactive Publication Date: 2019-08-30
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bupleurum is one of the most commonly used drugs in antidepressant compounds, and it has been reported in the literature that Bu

Method used

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  • Application of saikosaponin B2 in preparation of antidepressant drug
  • Application of saikosaponin B2 in preparation of antidepressant drug
  • Application of saikosaponin B2 in preparation of antidepressant drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: In vitro antidepressant activity test of saikosaponin B2

[0020] In this part of the experiment, PC12 cells were injured by corticosterone to simulate nerve cell damage caused by hyperactivity of the HPA axis in depression, and the protective effect of saikosaponin B2 on the cell damage model was investigated, and then its antidepressant activity was evaluated.

[0021] 1. Experimental materials: poorly differentiated PC12 cells (purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences). Saikosaponin B2 glycoside reference substance was purchased from Chengdu Pufeide Biotechnology Co., Ltd. (pure≧98%); RPMI-1640 medium (HyClone company); fetal bovine serum (Gibco company); MTT, poly-L-lysine Propidium iodide (PLL), DMSO (Sigma Company); Annexin V-FITC / propidium iodide (PI) apoptosis kit (BD Company); corticosterone (SIGMA Company); LDH detection kit (Nanjing Jiancheng Company).

[0022] 2. Test method...

Embodiment 2

[0030] Example 2: In vivo antidepressant efficacy test of saikosaponin B2

[0031] This part of the experiment evaluates the antidepressant effect of saikosaponin B2 in vivo through the classic mouse behavioral despair model (tail suspension model, forced swimming model).

[0032] 1. Experimental materials and grouping: male ICR mice, weighing 18-22 g, provided by Beijing Weitong Lihua Animal Experiment Center. Each group of 10 animals / cage is raised at room temperature (20 ± 2) ℃, humidity 60%, receives 12 h light / 12 h dark every day, and the photoperiod is 6:00-18:00, free access to standard feed and clean drinking water, animals The experiments complied with the international ethical requirements for animal experiments.

[0033] The normal mice were randomly divided into 5 groups according to body weight, 12 in each group, namely blank control group, positive drug fluoxetine (10 mg / kg), saikosaponin B2 high (40 mg / kg), medium (20 mg / kg) kg), low (10 mg / kg) dose group. Ea...

Embodiment 3

[0047] Example 3: Preparation of saikosaponin B2 soft capsules: Take 10 g of saikosaponin B2 and 190 g of vegetable oil, stir, mix well, and use as the contents for later use. Take appropriate amount of glycerin, gelatin and water (1:3:3 ratio), add appropriate amount of water to the gelatin to make it fully absorb water and swell, mix glycerin and the rest of the water, put it into the plastic barrel and heat to 70~80 ℃ , add an appropriate amount of expanded gelatin and propyl p-hydroxybenzoate, stir, and use it as a capsule material for later use. The contents and capsule materials are pressed into soft capsules on a soft capsule machine, dried and shaped to obtain saikosaponin B2 soft capsules.

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Abstract

The invention belongs to the technical field of biopharmaceutics and provides an application of saikosaponin B2 in preparation of an antidepressant drug. A structural formula of saikosaponin B2 is asshown in the specification, and saikosaponin B2 is a unique active ingredient in the antidepressant drug. In-vitro activity tests prove that saikosaponin B2 has better protection activity for PC12 injured by corticosterone, and in-vivo test results show that saikosaponin B2 can significantly shorten immobile time of mice in forced swimming and tail suspension tests. Test results show that saikosaponin B2 has a significant antidepressant effect and can be used for preparing a drug for preventing and treating depression.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of saikosaponin B2 in the preparation of antidepressant drugs. Background technique [0002] Depression is characterized by persistent low mood and cognitive dysfunction, including emotional agitation, anhedonia, sleep disturbance, guilt, and repeated suicidal thoughts, etc. It has high prevalence, high recurrence rate, high disability rate and Features such as high fatality rate. According to the statistics of the World Health Organization, more than 350 million people worldwide suffer from depression. By 2020, depression may become the second largest disease after heart disease. With the rapid development of social economy and the acceleration of the pace of life, people's pressure increases and various emotional impacts increase, resulting in an increase in the incidence of depression year by year. Depression is called "the epidemic of the 21s...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P25/24
CPCA61K31/704A61P25/24
Inventor 周玉枝李肖秦雪梅张丽增高晓霞田俊生
Owner SHANXI UNIV
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