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Application of cyperus rotundus extract in reducing skin inflammatory response caused by keratinocyte irradiation ultraviolet light and promoting skin keratin metabolism

A technology of keratinocytes and water-scented ribs, which is applied in skin care preparations, skin diseases, drug combinations, etc., and can solve problems such as spots, damage, and decline

Active Publication Date: 2019-08-30
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to restore keratin metabolism to normal, there are currently many exfoliating ingredients and methods on the market. However, after artificial exfoliation, although the skin will become smooth and shiny, the skin will become fragile due to the thinning of the stratum corneum , Sensitive and decreased water retention capacity
[0004] In addition, UVB in ultraviolet light has high energy, which can penetrate into the epidermis of the skin and cause sunburn, and cause symptoms such as hypertrophy of the epidermis, thickening of the stratum corneum, redness, swelling and inflammation, and once the skin is severely sunburned, the skin in the area will recover , will also become more fragile and prone to spots due to damage
[0005] In order to improve the current treatment methods for poor metabolism of old waste keratinocytes; skin redness, inflammation, and thickening of the stratum corneum after ultraviolet light irradiation; and thinning and damage of the stratum corneum, resulting in fragile, sensitive and rough skin, a There is a real need for a medicinal composition that promotes natural skin rejuvenation that can promote keratinocyte metabolism, anti-inflammation and increase skin protection

Method used

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  • Application of cyperus rotundus extract in reducing skin inflammatory response caused by keratinocyte irradiation ultraviolet light and promoting skin keratin metabolism
  • Application of cyperus rotundus extract in reducing skin inflammatory response caused by keratinocyte irradiation ultraviolet light and promoting skin keratin metabolism
  • Application of cyperus rotundus extract in reducing skin inflammatory response caused by keratinocyte irradiation ultraviolet light and promoting skin keratin metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of embodiment 1 Shuixiang rib extract of the present invention

[0021] Cyperus rotundus is a plant of the Cyperaceae family, also known as Cyperus rotundus, Cyperus rotundus, Tuxiang, stinky head incense, etc. Cyperus rotundus is known to have the effects of relieving pain, regulating menstruation, dispelling cold and hernia, and relieving abdominal pain .

[0022] In one embodiment of the present invention, the whole plant of Shuixianglong is washed, and the extracted solvent of the washed whole Shuixianglong plant and water, alcohol, or a mixture of alcohol and water is used at a liquid-solid ratio of 5-20:1-5 After mixing and homogenizing, extract at 50-100° C., preferably 75° C.-95° C., for 0.5-3 hours to obtain a crude extract. After cooling to room temperature after extraction, the crude extract was filtered through a 400-mesh filter to obtain a filtrate. Finally, the filtrate is concentrated under reduced pressure at 45-70° C. to obtain ...

Embodiment 2

[0023] Embodiment 2 The effect of Shuixiang rib extract of the present invention on promoting the hyperplasia of skin keratinocytes

[0024] The present invention uses human primary epidermal keratinocytes (human primary epidermal keratinocytes, HPEKp) to carry out the experiment of skin keratinocyte hyperplasia. The human primary skin keratinocytes were purchased from CELLnTEC (Switzerland) No. HPEK-50. The cells were cultured in serum-free keratinocyte medium (keratinocyte-SFM) (Gibco, USA, number #10724-011).

[0025] The cell proliferation reagent kit was used for analysis (purchased from Roche Company, Switzerland, No. 11647229001). First, in a 96-well culture dish, 3×10 3 Individual human primary skin keratinocytes were cultured at 37°C for 2 hours with the above-mentioned culture solution, and 100 μl of blank control group (mock) or 1.25 mg / mL Shuixiang prism extract of the present invention was added to each well, and 10 μl of 100 μM brominated deoxygenated Uridine ...

Embodiment 3

[0027] Example 3 The Shuixiang rib extract of the present invention inhibits the secretion of interleukin 8 (interleukin-8, IL-8) due to ultraviolet radiation

[0028] Since it is known that interleukin-8 (interleukin-8, IL-8) is a cytokine, it has the function of promoting the generation of inflammatory response, therefore, the present invention further detects the ability of Shuixiang rib extract to inhibit the secretion of interleukin-8, This secretion is caused by irradiation of keratinocytes with ultraviolet rays. First, add 500 μl of the above-mentioned culture solution to each well of a 24-well culture plate and implant 5x10 4 keratinocytes and cultured at 37°C for 24 hours. The culture solution was removed without disturbing the attached cells, and divided into four groups to treat keratinocytes (n=3), respectively (1) the blank control group (mock) added culture solution and did not irradiate UVB, (2) normal In the control group, the keratinocytes were added to the ...

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Abstract

The invention relates to an application of a cyperus rotundus extract, in particular to an application of the cyperus rotundus extract in reducing skin inflammatory response caused by keratinocyte irradiation ultraviolet light and promoting skin keratin metabolism. The cyperus rotundus extract is obtained by extracting water-fragrant edges with water, alcohol or a solvent of an alcohol-water mixture, and can reduce skin inflammatory reaction of skin keratinocytes caused by irradiation of ultraviolet light, promote skin keratinocyte metabolism, improve skin texture and pore size and reduce melanin content.

Description

technical field [0001] The present invention relates to the use of the extract of Shuixiang, especially the use of the extract of Shuixiang for preparing a pharmaceutical composition for reducing skin inflammation caused by keratinocytes irradiated with ultraviolet light and promoting skin keratin metabolism. Background technique [0002] The stratum corneum is located in the outermost layer of the skin epidermis, and its main function is to keep the skin water and help the skin resist various environmental damages. The stratum corneum is composed of 15 to 20 layers of keratinocytes. Keratinocytes are dead cells without nuclei and organelles. The main component of keratin is keratin, which absorbs water and keeps the skin moist. Keratinocytes will fall off by themselves after a period of time, and then the cells below the stratum corneum will be keratinized and pushed upward to form a new stratum corneum. [0003] The old waste cells in the stratum corneum will fall off by ...

Claims

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Application Information

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IPC IPC(8): A61K36/8905A61P17/00A61P17/16A61K8/9794A61Q19/00A61Q19/08A61Q17/04
CPCA61K36/8905A61P17/00A61P17/16A61K8/9794A61Q19/00A61Q19/08A61Q17/04A61K2236/331A61K2236/333
Inventor 林咏翔张蓉
Owner 百岳特生物技术(上海)有限公司
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