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The use of water fragrant edge extract to reduce skin inflammation caused by keratinocytes exposed to ultraviolet light and promote skin keratin metabolism

A technology of skin cutin and cutin cells, applied in skin care preparations, skin diseases, drug combinations, etc., can solve problems such as sensitive water retention capacity, skin fragility, and thickened cuticle

Active Publication Date: 2022-08-02
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to restore keratin metabolism to normal, there are currently many exfoliating ingredients and methods on the market. However, after artificial exfoliation, although the skin will become smooth and shiny, the skin will become fragile due to the thinning of the stratum corneum , Sensitive and decreased water retention capacity
[0004] In addition, UVB in ultraviolet light has high energy, which can penetrate into the epidermis of the skin and cause sunburn, and cause symptoms such as hypertrophy of the epidermis, thickening of the stratum corneum, redness, swelling and inflammation, and once the skin is severely sunburned, the skin in the area will recover , will also become more fragile and prone to spots due to damage
[0005] In order to improve the current treatment methods for poor metabolism of old waste keratinocytes; skin redness, inflammation, and thickening of the stratum corneum after ultraviolet light irradiation; and thinning and damage of the stratum corneum, resulting in fragile, sensitive and rough skin, a There is a real need for a medicinal composition that promotes natural skin rejuvenation that can promote keratinocyte metabolism, anti-inflammation and increase skin protection

Method used

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  • The use of water fragrant edge extract to reduce skin inflammation caused by keratinocytes exposed to ultraviolet light and promote skin keratin metabolism
  • The use of water fragrant edge extract to reduce skin inflammation caused by keratinocytes exposed to ultraviolet light and promote skin keratin metabolism
  • The use of water fragrant edge extract to reduce skin inflammation caused by keratinocytes exposed to ultraviolet light and promote skin keratin metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 The preparation method of the water fragrant edge extract of the present invention

[0021] Cyperusrotundus (Cyperusrotundus) is a plant of Cyperaceae, also known as Cyperus officinalis, fragrant head grass, earth incense, stinky head incense, etc., water fragrant edge is known to have the effects of relieving pain, regulating menstruation, dispelling cold hernia and relieving abdominal pain. .

[0022] In one embodiment of the present invention, the whole plant of S. serrata is washed, and the extraction solvent of the whole plant of S. serrata after washing and water, alcohol, or mixture of alcohol and water is taken at a liquid-solid ratio of 5-20: 1-5 Mix and homogenize, and perform extraction at 50-100°C, preferably 75°C-95°C, for 0.5-3 hours to obtain a crude extract. After extraction, it was cooled to room temperature, and the crude extract was filtered through a 400-mesh sieve to obtain a filtrate. Finally, the filtrate is concentrated under reduc...

Embodiment 2

[0023] Example 2 The effect of the water fragrant edge extract of the present invention on promoting the proliferation of skin keratinocytes

[0024] The invention uses human primary epidermal keratinocytes (human primary epidermal keratinocytes, HPEKp) to carry out the experiment of skin keratinocyte proliferation. The human primary skin keratinocytes were purchased from CELLnTEC (Switzerland) under the number HPEK-50. The cells were cultured in serum-free keratinocyte-SFM (Gibco, USA, No. #10724-011).

[0025] The analysis was performed using the Cell Proliferation Reagent Kit (purchased from Roche, Switzerland, no. 11647229001). First, in a 96-well culture dish, each well was seeded with 3 × 10 3 Individual human primary skin keratinocytes were cultured with the above-mentioned culture medium at 37°C for 2 hours, and 100 μl of blank control group (mock) or 1.25 mg / mL of the extract of the present invention, and 10 μl of 100 μM brominated deoxygenation were added to each w...

Embodiment 3

[0027] Example 3 The water-flavor extract of the present invention inhibits the secretion of interleukin-8 (IL-8) due to ultraviolet irradiation

[0028] Since it is known that interleukin-8 (IL-8) is a cytokine and has the function of promoting the production of inflammatory response, the present invention further detects the ability of the Shuixiangleng extract to inhibit the secretion of interleukin-8, This secretion is caused by exposure of keratinocytes to ultraviolet light. First, in a 24-well culture dish, add 500 μl of the above culture medium to each well and implant 5×10 4 keratinocytes were cultured at 37°C for 24 hours. The culture medium was removed without disturbing the attached cells, and the keratinocytes were divided into four groups (n=3), respectively (1) the blank control group (mock) added culture medium and did not irradiate UVB, (2) positive In the control group, keratinocytes were added to the culture medium and incubated at 37°C for 2 hours and then...

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Abstract

The present invention relates to the use of the water fragrant edge extract, especially the use of the water fragrant edge extract for reducing skin inflammation caused by keratinocyte irradiation with ultraviolet light and promoting skin keratin metabolism. The water fragrant edge extract is obtained by extracting water fragrant edge with a solvent of water, alcohol, or alcohol-water mixture, which can reduce skin keratinocyte inflammation caused by ultraviolet light irradiation, promote skin keratin metabolism and improve skin texture, Pore ​​size and reduced melanin content.

Description

technical field [0001] The present invention relates to the use of the water fragrant edge extract, especially the use of a water fragrant edge extract for preparing a pharmaceutical composition for reducing skin inflammation caused by keratinocyte irradiation with ultraviolet light and promoting skin keratin metabolism. Background technique [0002] The stratum corneum is located in the outermost layer of the skin epidermis, and its main function is to keep the skin hydrated and help the skin resist various environmental damages. The stratum corneum consists of 15 to 20 layers of keratinocytes. The keratinocytes are dead cells without nuclei and organelles. The main component is keratin, which can absorb water and keep the skin moist. After a period of time, the keratinocytes will fall off by themselves, and then the cells below the stratum corneum will be keratinized and pushed upward to form a new stratum corneum. [0003] The old and waste cells in the stratum corneum w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8905A61P17/00A61P17/16A61K8/9794A61Q19/00A61Q19/08A61Q17/04
CPCA61K36/8905A61P17/00A61P17/16A61K8/9794A61Q19/00A61Q19/08A61Q17/04A61K2236/331A61K2236/333
Inventor 林咏翔张蓉
Owner 百岳特生物技术(上海)有限公司
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