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Paeoniflorin molecularly imprinted polymer as well as preparation and application thereof

A technology of molecular imprinting and paeoniflorin, applied in ion exchange, other chemical processes, ion exchange regeneration, etc., can solve problems such as few literature studies, and achieve the effect of easy large-scale production and high purity

Active Publication Date: 2019-08-30
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of molecular imprinting technology to iridoid glycosides has been relatively mature, but there are few literature studies on the application of molecular imprinting technology to paeoniflorin

Method used

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  • Paeoniflorin molecularly imprinted polymer as well as preparation and application thereof
  • Paeoniflorin molecularly imprinted polymer as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of paeoniflorin molecularly imprinted polymer

[0019] (1) Prepare paeoniflorin molecularly imprinted polymer by sol-gel method, the specific process is as follows: Weigh 200g of spherical silica gel with a particle size of about 50μm, disperse it in 10ml of absolute ethanol, and then add 3ml (1.56mmol) of cross-linking agent TEOS , functional monomer APTES (0.86mmol) and PTMOS (1.0mmol) each 200μL, after shaking at room temperature for 30min, add 0.1mol / L paeoniflorin (0.08mmol) ethanol solution 800μL, twice distilled water 1ml, 100μL concentrated HCl (concentration 36 %), reacted at room temperature for 4h, filtered to obtain 5.6g of filter cake. The filter cake was washed three times with absolute ethanol, dried at 60°C, and then the filter cake was eluted with methanol-acetic acid (9:1, v / v) to remove template molecules in the polymer until the washing liquid had no UV absorption at 230nm. The imprinted polymer was washed three times with methanol to...

Embodiment 2

[0025] 1. Preparation of paeoniflorin molecularly imprinted polymer

[0026] (1) The molecularly imprinted polymer of paeoniflorin was prepared by sol-gel method. The specific process is as follows: Weigh 200g of spherical silica gel with a particle size of about 50μm, disperse it in 10ml of absolute ethanol, and then add 6ml (3.12mmol) of cross-linking agent TEOS , functional monomer APTES1 (1.72mmol) and PTMOS (2.0mmol) each 400μL, shake at room temperature for 30min, add 0.1mol / L paeoniflorin (0.16mmol) ethanol solution 1.6mL, twice distilled water 1ml, 200μL concentrated HCl, room temperature The reaction was carried out for 4h to obtain 3.3g of the product. The product was first washed three times with absolute ethanol and dried. Then, the template molecules in the polymer were eluted with methanol-acetic acid (9:1v / v) until the washing solution had no UV absorption at 230nm, and the imprinted polymer was washed with methanol three times to remove residual acetic acid. ...

Embodiment 3

[0030] 1. Preparation of paeoniflorin molecularly imprinted polymer

[0031](1) The molecularly imprinted polymer of paeoniflorin was prepared by the sol-gel method. The specific process is as follows: Weigh 200 g of spherical silica gel with a particle size of about 50 μm, disperse it in 10 ml of absolute ethanol, and then add 1.5 ml of cross-linking agent TEOS (0.78 mmol), functional monomer APTES1 (0.43mmol) and PTMOS (0.5mmol) each 100μL, after shaking at room temperature for 30min, add 0.1mol / L paeoniflorin (0.04mmol) ethanol solution 400μL, twice distilled water 1ml, 50μL concentrated HCl, The reaction was carried out at room temperature for 4 hours to obtain 4.4 g of the product. The product was first washed three times with absolute ethanol and dried. Then use methanol-acetic acid (9:1v / v) to elute the template molecules in the polymer until the washing solution has no UV absorption at 230nm. The imprinted polymer was washed three times with methanol to remove residu...

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Abstract

The invention discloses a paeoniflorin molecularly imprinted polymer as well as preparation and application thereof. The polymer is prepared by the following steps: taking silica gel, dispersing the taken silica gel into anhydrous ethanol, adding a crosslinking agent, a functional monomer and phenyltrimethoxysilane, performing shaking at room temperature for 30 min, adding a paeoniflorin ethanol solution, redistilled water and concentrated HCl, performing a reaction at room temperature for 4 h, performing filtration, washing the filter cake by using absolute ethanol, performing drying, performing washing by using methanol-acetic acid with a volume ratio of methanol to acetic acid of 9:1 until a washing liquid has no ultraviolet absorption at 230 nm, performing washing by using methanol toremove residual acetic acid, and finally performing drying to a constant weight to obtain the paeoniflorin molecularly imprinted polymer. The method provided by the invention adopts a sol-gel processto prepare the paeoniflorin molecularly imprinted polymer with double functional monomers, can well enrich paeoniflorin from a paeonia lactiflora flower extraction liquid and remove other impurities in the paeonia lactiflora flower extraction liquid, wherein the enrichment rate can be up to 90%.

Description

Background technique [0001] Peony is the dried root of the perennial herb Paeoniae Paeoniae, bitter, sour, slightly cold, returns to the liver and spleen meridian, has the functions of calming the liver and relieving pain, nourishing blood and regulating menstruation, astringing yin and stopping sweating. For headache, dizziness, rib pain, abdominal pain, spasm and pain in limbs, blood deficiency and chlorosis, irregular menstruation, spontaneous sweating, and night sweating. It grows in bushes or grasses on hillsides and valleys. Mainly distributed in Anhui, Heilongjiang, Jilin, Liaoning, Hebei, Henan, Shandong, Shanxi, Shaanxi, Inner Mongolia and other places, it is now cultivated all over the country. At home and abroad, there are many studies on the chemical components, pharmacological activity and clinical application of peony, and a lot of exploration and research have been done on the extraction process of the active ingredients in peony. The root of Radix Paeoniae Al...

Claims

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Application Information

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IPC IPC(8): C08G77/26C08G77/06B01J20/26B01J20/30B01D15/08
CPCC08G77/26C08G77/06B01J20/268B01D15/08B01J2220/4806B01J2220/4812
Inventor 张诚郑晓周王平
Owner ZHEJIANG UNIV OF TECH
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