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A Chromium-resistant Petroleum Hydrocarbon Degrading Bacteria tph2-23 and Its Application

A petroleum hydrocarbon degrading bacteria, chromium-resistant technology, applied in the direction of bacteria, microbial-based methods, restoration of contaminated soil, etc.

Active Publication Date: 2021-09-28
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the combined pollution of petroleum hydrocarbon pollutants and heavy metals mainly focuses on the ecotoxicological effects.
The reported in-situ remediation technologies for organic matter-heavy metal complex pollution include electrokinetic enhanced remediation, microbial remediation, phytoremediation, and combined chemical-biological technologies (chemical oxidation-microbial degradation, chemical reduction-hyperaccumulation plant enrichment, and chemical passivator-phytoremediation). Repair), etc., there is no relevant report specifically for diesel-Cr 6+ Microbial remediation technology of petroleum hydrocarbon pollutants represented by complex pollution-toxic heavy metals

Method used

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  • A Chromium-resistant Petroleum Hydrocarbon Degrading Bacteria tph2-23 and Its Application
  • A Chromium-resistant Petroleum Hydrocarbon Degrading Bacteria tph2-23 and Its Application
  • A Chromium-resistant Petroleum Hydrocarbon Degrading Bacteria tph2-23 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Morphological characteristics of petroleum hydrocarbon degrading bacteria (Achromobacter insolitus) Tph2-23

[0020] Streak inoculate a single colony into LB solid medium, place the plate upside down in a constant temperature incubator, and incubate at 28°C for 48 hours. Protrusion, diameter 0.5 ~ 1mm, see figure 1 .

Embodiment 2

[0021] Example 2: Screening and Identification of Achromobacter insolitus Tph2-23

[0022] (1) Screened from a petroleum hydrocarbon-contaminated area (E113°55′, N22°20′) of a Macau power plant, the soil samples were aseptically collected, subpackaged, sealed, and transported to the laboratory at low temperature for the next stage research work.

[0023] (2) Under laboratory conditions: take a 250mL Erlenmeyer flask, wash it, fill it with 90mL deionized water and add 25 glass beads with a diameter of 0.5cm, seal it and place it in an autoclave, and extinguish it under high temperature and high pressure at 121°C. Bacteria 20min.

[0024] (3) After the above system is lowered to room temperature, add 10.0 g of the aforementioned soil samples into the Erlenmeyer flask in a sterile ultra-clean workbench. The microorganisms were completely separated out. The suspension produced by the above process is the stock solution of gradient dilution. The stock solution was equally dilut...

Embodiment 3

[0026] Embodiment 3: Petroleum hydrocarbon degrading bacteria (Achromobacter insolitus) Tph2-23 is to Cr 6+ and Determination of Diesel Degradation Rate

[0027] (1) Prepare LB liquid medium, and pick a single colony of petroleum hydrocarbon degrading bacteria (Achromobacterinsolitus) Tph2-23 on the LB solid plate to inoculate in the LB liquid medium, cultivate overnight, which is the Tph2-23 seed solution.

[0028] (2) After sterilizing 10g of diesel oil and 90mL of inorganic salt liquid medium, mix them separately, and add potassium dichromate powder dried to constant weight to make the Cr in the system 6+ About 50mg / L. Insert the strain seed liquid at a ratio of 1%, cultivate it at 28°C and 150rpm for 15 days, take samples every 3 days, and measure Cr 6+ and diesel content, and calculate the degradation rate at the corresponding time point.

[0029] (3) The results show that: Achromobacter insolitus Tph2-23 has the effect on Cr 6+ The total degradation rate reaches 88.7...

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Abstract

A chromium-resistant petroleum hydrocarbon degrading bacterium Tph2‑23 and its application relate to petroleum hydrocarbon degrading bacteria. It was deposited in the China Center for Type Culture Collection on November 12, 2018, and the deposit number of the collection center is CCTCC NO: M 2018786. Provide a chromium-resistant petroleum hydrocarbon degrading bacteria (Achromobacter insolitus) Tph2‑23 in the treatment of diesel oil and heavy metal Cr 6+ Application in contaminated soil remediation. The isolation and application of Achromobacter insolitus Tph2‑23 effectively filled the research gap in this area, and provided a set of feasible practical solutions for the treatment of actual polluted environments, showing great practical application prospects.

Description

technical field [0001] The invention relates to petroleum hydrocarbon degrading bacteria, in particular to a chromium-resistant petroleum hydrocarbon degrading bacteria (Achromobacter insolitus) Tph2-23 and application thereof. Background technique [0002] Diesel oil is a light petroleum product, a mixture of complex hydrocarbons (about 10-22 carbon atoms), and is divided into two categories: light diesel oil (boiling point range is about 180-370°C) and heavy diesel oil (boiling point range is about 350-410°C). The most important use of diesel is in diesel engines for vehicles and ships. Petroleum hydrocarbon pollutants represented by diesel oil will inevitably pollute the environment during transportation, loading and unloading, processing and use. They are flammable, and the exhaust gas produced after combustion contains nitrogen oxides, carbon monoxide, carbon dioxide, aldehydes and a large amount of black smoke, which will cause serious pollution to the atmospheric env...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/025
CPCB09C1/10C12N1/205C12R2001/025
Inventor 赵苒唐晨王万鹏郭东北范春张皓璠李佳瑶许婉婷李晓夏李怡
Owner XIAMEN UNIV