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Application of Cyclocarya paliurus extract in preparation of medicine for treating or preventing diabetic cardiomyopathy

A technology of diabetic cardiomyopathy and its extract, which is applied in the field of application of Cyclocarya paliurus extract in the preparation of drugs for the treatment of diabetic cardiomyopathy, and can solve the problems of no patents or literature reports

Active Publication Date: 2021-06-18
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there are no patents or literature reports on the research results of the water extract of Cyclocarya paliurus in the treatment of diabetic cardiomyopathy

Method used

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  • Application of Cyclocarya paliurus extract in preparation of medicine for treating or preventing diabetic cardiomyopathy
  • Application of Cyclocarya paliurus extract in preparation of medicine for treating or preventing diabetic cardiomyopathy
  • Application of Cyclocarya paliurus extract in preparation of medicine for treating or preventing diabetic cardiomyopathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0023] Implementation case one Cyclocarya paliurus extract preparation

[0024] Weigh 35 kg of dried Cyclocarya paliurus, heat and reflux extract twice with 70% ethanol at 10 times the amount, for 2 hours each time, filter while hot, and combine the filtrates. Recover the solvent under reduced pressure until there is no alcohol smell, add water to disperse, and obtain the sample solution. Use D101 type macroporous resin to pack the column according to the diameter-to-height ratio of 1:4, the loading concentration (extract volume: solution volume) is 1:10, the volume ratio of extract volume to resin is 1:7, and the adsorption flow rate is 0.5BV / h, elution flow rate 2BV / h, wash 8BV to remove impurities, 30% ethanol to elute 10BV, 50% ethanol to elute 10BV, 75% ethanol to elute 10BV, collect 30%-75% ethanol eluate, recover solvent, vacuum After drying and weighing, 1.8 kg of the effective part of Cyclocarya paliurus was obtained.

[0025] The willow extract is applied to the p...

Embodiment example 2

[0029] Example 2 Determination of fasting blood sugar, total cholesterol and triglycerides

[0030] 48 6-week-old male db / db spontaneously diabetic mice were randomly divided into 5 groups: high, medium and low doses of total triterpenoids (ECL) of Cyclocarya paliurus were given ECL 80, 40, 20 mg·kg respectively. -1 d -1 Oral administration of the suspension; the model group was given the same dose of 0.9% normal saline 0.2mL. d -1 Gavage; the normal group did not intervene. Continuous intervention for 10 weeks. The fasting blood glucose of each group was detected before administration and at the 2nd, 4th, 6th, 8th, and 10th weekend after administration.

[0031] After the experimental administration, the mice were fasted for 12 hours, and blood was taken from the heart, 3500r·min -1Serum was collected by centrifugation for 10 min, and stored at 4°C for biochemical index detection. Fasting blood glucose was tested using a Roche blood glucose meter, and the content of tri...

Embodiment example 3

[0032] Implementation Case 3 Biochemical Index Detection

[0033] According to the kit instructions, the levels of LDH, CK, AST cardiac three enzymes, SOD, CAT, MDA, and GSH-PX were measured respectively, and cardiac troponin (cTnl) and inflammatory factors IFNγ, IL-1β, MCP-1, TNFα levels. Myocardial tissue was added with physiological saline at a mass volume ratio of 1:9, and after homogenization, 3 000r·min -1 After centrifugation for 10 min, the supernatant was taken to determine the levels of TGFβ1 and Collagen I according to the kit instructions.

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Abstract

The invention discloses the application of Cyclocarya paliurana extract in the preparation of medicines for treating or preventing diabetic cardiomyopathy. The medicine includes Cyclocarya paliurus extract and a pharmaceutically acceptable carrier. It is the alcohol extract of Cyclocarurus paliurus. The beneficial effects of the present invention are: the alcohol extract of Cyclocarya paliurus can reduce hypoglycemia, serum total cholesterol and triglyceride in mice; reduce the release of LDH, CK and AST myocardial three enzymes due to myocardial cell necrosis during acute myocardial infarction, and Amount of necrosis marker cardiac troponin. Cyclocarurus alcohol extract can significantly improve the structure and shape of diabetic cardiomyocytes, and reduce the apoptosis of cardiomyocytes.

Description

technical field [0001] The invention relates to a traditional Chinese medicine, in particular to the application of an extract of Cyclocarya paliurus in the preparation of medicine for treating diabetic cardiomyopathy. Background technique [0002] The incidence of diabetes is increasing year by year, and cardiovascular complications secondary to diabetes have become the main cause of death in diabetic patients. More than 70% of diabetic patients die from cardiovascular system diseases, which is 2 to 3 times the mortality rate of cardiovascular system diseases in non-diabetic populations. Among them, diabetic cardiomyopathy (diabetic cardiomyopathy, DCM) is one of the main complications of diabetic patients. As the number of diabetic patients increases year by year, the incidence of cardiomyopathy in diabetic patients also increases rapidly. Hypertrophy is the main manifestation, and long-term development can also lead to myocardial fibrosis and heart failure. [0003] Cyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/52A61P3/10A61P9/00
CPCA61K36/52A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P3/10A61P9/00
Inventor 朱寅荻郑晓杰王朝杰胡臻汪洪
Owner WENZHOU MEDICAL UNIV
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