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Application of swim bladder polypeptide

A technology of swim bladder and oxidative damage, applied in the field of biochemical pharmacy, can solve problems such as lack of treatment methods, and achieve the effect of restoring low fertility and lowering cholesterol

Pending Publication Date: 2019-09-06
上海拜汶生物医药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenesis of oligoasthenospermia is complex and diverse, among which testicular oxidative damage and germ cell apoptosis are important causes of oligoasthenospermia, and there is still a lack of targeted treatment in clinical practice

Method used

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  • Application of swim bladder polypeptide
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  • Application of swim bladder polypeptide

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045](1-1) Preparation of the model group, using testicular injection of busulfan: randomly select 8-week-old sexually mature male mice, and perform general anesthesia by intraperitoneal injection of pentobarbital sodium, keep the mice in a supine position, and remove the testis from the Gently squeeze the abdominal cavity into the scrotum, then disinfect the scrotum with 75% alcohol, fix the testicle on one side with the thumb and index finger of the left hand, and hold the micro-injector in the right hand to avoid the blood vessels on the testicular albuginea and directly penetrate the skin of the scrotum to enter the testis, push the syringe Busulfan is injected into one testicle, and the same is done to the other testicle. The dose of busulfan was 4mg / kg, and the model of oligoasthenospermia was established.

[0046] (1-2) Preparation of swim bladder polypeptide 30 mg / kg and 60 mg / kg groups: swim bladder polypeptide 30 mg / kg and 60 mg / kg groups were administered by intrag...

Embodiment 1

[0051] drug dose selection

[0052] Experimental mice: ICR mice of SPF grade, provided by the Animal Experiment Center of Nantong University, license number: SYXK9 (Su) 2002-0022, the experimental animals follow the standard operating procedures, and have been reviewed by Nantong University for experimental animal ethics.

[0053] Swim bladder polypeptide dose selection: Materials: We first obtained the crude swim bladder extract of large yellow croaker from Shanghai University of Traditional Chinese Medicine, and then hydrolyzed the crude swim bladder extract with alkaline protease. The obtained enzymolysis product is subjected to ultrafiltration to obtain the swim bladder polypeptide with a molecular weight less than 10kD.

[0054] 1) Take 8-week-old male ICR mice.

[0055] 2) Divide into four groups randomly, with 6 mice in each group.

[0056] Normal control group, model group, swim bladder polypeptide 30mg / kg group, swim bladder polypeptide 60mg / kg group.

[0057] Swim...

Embodiment 2

[0060] Fertility Index Detection of Mice Treated with Busulfan and Swim Bladder Polypeptides

[0061] experimental method:

[0062] Experimental Animal Handling

[0063] 1) Take 8-week-old male ICR mice.

[0064] 2) Divide into four groups randomly, with 6 mice in each group.

[0065] The normal control group was injected with normal saline into the testes.

[0066] In the model group, busulfan was injected into the testes once at a dose of 4 mg / kg.

[0067] For the swim bladder polypeptide 30 mg / kg group, the testicular injection of busulfan 4 mg / kg was given once, and the swim bladder polypeptide 30 mg / kg was given continuously by intragastric administration for 28 days.

[0068] For the swim bladder polypeptide 60 mg / kg group, the testicular injection of busulfan 4 mg / kg was given once, and the swim bladder polypeptide 60 mg / kg was given continuously by intragastric administration for 28 days.

[0069] 3) After 28 days of swim bladder polypeptide treatment, the mice we...

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Abstract

The invention belongs to the field of biochemical pharmacy and provides application of a swim bladder polypeptide to medicines for inhibiting oxidative damage and cell apoptosis in human bodies or animal bodies. The oxidative damage refer to testicle oxidative damage, the cell apoptosis refers to testicular spermatogenic cell apoptosis, the oxidative damage and the cell apoptosis are caused by antitumor drugs including busulfan, cyclophosphamide and thiotepa, and busulfan is used as the antitumor drug in an embodiment. The swim bladder polypeptide is capable of raising the expression level ofa transcription factor Nrf2, reducing an apoptosis promoting factor Bax and raising the expression level of an anti-apoptosis factor Bcl2; the swim bladder polypeptide is capable of relieving busulfaninduced germ cell vacuolization, spermatogenic cell apoptosis, seminiferous tubule degradation and sperm amount and viability reduction. By adoption of the swim bladder polypeptide for preparation ofthe medicines for inhibiting oxidative damage and cell apoptosis, medicines for recovering fertility of men or male animals can be prepared, and a direction is provided for treatment of fertility ofmen or male animals.

Description

technical field [0001] The invention belongs to the field of biochemical pharmacy, and relates to the application of swim bladder polypeptides, in particular to the use of swim bladder polypeptides in medicines for inhibiting oxidative damage and apoptosis in human or animal bodies and the use of swim bladder polypeptides in preparing medicines for restoring the fertility of human males or male animals In particular, it relates to the effective inhibition of testicular oxidative damage and germ cell apoptosis induced by Busulfan, and its drug application in restoring and improving male fertility. Background technique [0002] In recent years, with social development, excessive living pressure and aggravation of environmental pollution, the rate of infertility has increased significantly. According to the report of the World Health Organization, the incidence of infertility among couples of normal childbearing age is nearly 15%, and the male factor accounts for about 50%, mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61P15/00A61P15/08A61P39/00
CPCA61K38/012A61P15/00A61P15/08A61P39/00
Inventor 孙斐赵喜龚胜男刘振华
Owner 上海拜汶生物医药股份有限公司
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