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In vivo single-cell multifunctional spectrometer based on coaxial dual-waveguide fiber

A multi-function spectrometer and dual-waveguide technology, which is applied in the field of single-cell multi-function spectrometers in vivo, can solve the problems of hindered concentration difference and low substance content, and achieve rapid analysis in vivo, high integration, and high operational flexibility. Effect

Active Publication Date: 2021-09-28
GUILIN UNIV OF ELECTRONIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, single-cell analysis research was once hindered due to the extremely small volume of cells, a large number of substance types, very little substance content, and significant concentration differences between different substances.

Method used

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  • In vivo single-cell multifunctional spectrometer based on coaxial dual-waveguide fiber
  • In vivo single-cell multifunctional spectrometer based on coaxial dual-waveguide fiber
  • In vivo single-cell multifunctional spectrometer based on coaxial dual-waveguide fiber

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0051] The side throw coupler 18 can be used Image 6 The preparation method is prepared, and the prepared side throwing coupler has good long-term stability. The preparation steps are:

[0052] (1) Preparation of V-groove: According to the parameters of single-mode fiber and coaxial dual-waveguide fiber, determine the amount of side throwing, and prepare V-shaped quartz grooves of appropriate depth and shape according to the amount of side throwing;

[0053] (2) Fix the optical fiber and polish it: place the optical fiber stripped of a section of the coating layer in the groove, fix it with UV-curable glue with good stability, fix the two kinds of optical fiber in the V-groove of their respective parameters, and polish the optical fiber To the upper plane of the V-groove and polished;

[0054] (3) Coupling adjustment and glue dispensing packaging: using the method of real-time power monitoring through light, in a special packaging device, the coupling alignment adjustment a...

Embodiment 1

[0057] Example 1: Acquisition of single-cell mass spectrometry:

[0058] The wavelength of the capture beam 21 is 980nm, the wavelength of the interrogation beam 22 is 1310nm, and the two beams of light are jointly passed into the ring core of the coaxial dual waveguide fiber 7, such as Figure 8 (a) shown. The incoming 980nm capture beam 21 , the capture beam 20 is totally internally reflected at the truncated cone, and converges at a distance from the end face of the optical fiber to achieve a deep capture potential well, and single cells 20 are captured. Then, through the function generator 3, a cosine signal with a frequency continuously adjustable from 1 Hz to 1 GHz is given to the light intensity modulator 4, so that the captured light beam 21 captures the light intensity I in a quasi-static state. trap based on having an AC modulation with a cosine, such as Figure 8 (b) Shown on the left. The cosine-modulated light intensity can not only capture the single cell 20 q...

Embodiment 2

[0059] Example 2: Acquisition of single-cell Raman spectrum:

[0060] Such as Figure 9 As shown, the Raman excitation beam and the single-cell capture beam 21 are the same beam, and a 980nm capture light source is used. The light beam is coupled into the annular core of the coaxial dual waveguide fiber 7, and the light intensity modulator 4 controls the light intensity to be constant, such as Figure 9 (b) Shown on the left. The capture light beam 21 is reflected and converged by the frustum of the cone, not only to capture the single cell 20, but also to excite the Raman spectrum of the captured single cell 20 due to the high energy density of the converged capture beam 21. The backscattered Raman signal light 23 is collected and transmitted through the intermediate core of the coaxial dual-waveguide fiber 7, and after the Rayleigh scattered light is filtered out by the optical filter 14-1, it is input into the Raman spectrometer 15 for analysis to obtain its Raman signal ...

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Abstract

The present invention provides a live single cell multifunctional spectrometer based on coaxial dual-waveguide optical fiber, which is characterized in that it consists of a capture light source 1-1, an interrogation light source 1-2, a standard single-mode optical fiber 2, and a function generator 3. Light intensity modulator 4, isolator 5, power meter 6, coaxial double waveguide fiber 7, illumination source 8, stage 9, microscope objective lens 10, CCD11, computer 12, fiber coupler 13, optical filter 14‑1, 14‑2, Raman spectrometer 15, balanced photodetector 16, oscilloscope 17 with FFT files, side throwing coupler 18 and micromanipulator 19. The invention can be used for the acquisition of single cell capture, single cell Raman spectrum, single cell fluorescence spectrum and single cell mass spectrum, and can be widely used in the technical field of single cell operation and spectrum analysis.

Description

technical field [0001] The invention relates to a live single cell multifunctional spectrometer based on coaxial dual waveguide optical fiber, which can be used for single cell capture, mass spectrometry, Raman spectrum measurement and fluorescence spectrum measurement, and belongs to the technical field of single cell manipulation, measurement and analysis . Background technique [0002] Cells are the basic unit of living organisms. Understanding the life activities in a cell is of great value for us to understand the life process. Single-cell technology is the frontier of current biological technology, which can provide scientists with a lot of new biological information, not only to test the conclusions of past classical methods, but also to discover many new laws. Traditional cell biology classifies and studies cell populations based on their source tissue, their own external morphology, and secretions. These technologies and analysis methods have achieved remarkable r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/65G01N27/64
CPCG01N21/6486G01N21/65G01N27/64
Inventor 苑立波杨世泰邓洪昌
Owner GUILIN UNIV OF ELECTRONIC TECH
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