A kind of fluorescent carbon dot and its preparation method and application

A technology of fluorescent carbon dots and fluorescent probes, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problems of complex synthesis methods, poor water solubility, and limited applications, and achieve simple preparation methods, low cost, The effect of easy operation

Active Publication Date: 2020-11-24
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they still have some unavoidable disadvantages, such as complex synthesis methods, poor water solubility, and high toxicity, which limit their application in the biomedical field.

Method used

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  • A kind of fluorescent carbon dot and its preparation method and application
  • A kind of fluorescent carbon dot and its preparation method and application
  • A kind of fluorescent carbon dot and its preparation method and application

Examples

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preparation example Construction

[0030] A method for preparing fluorescent carbon dots that specifically recognize tryptophan, dispersing 3-aminoisonicotinic acid in ultrapure water to obtain ultrapure 3-aminoisonicotinic acid with a mass-volume ratio of 0.1-50 mg / mL water dispersion. The ultrapure water dispersion of 3-aminoisonicotinic acid is transferred into a polytetrafluoroethylene stainless steel high-pressure reactor, and placed in an oven for heating reaction. The reaction temperature is 120-200° C., and the reaction time is 6-12 hours. The reaction product was centrifuged at 10000rpm for 10min (centrifuged twice) and the supernatant was taken, and the supernatant was filtered with a 0.22 μm syringe filter membrane, and the resulting filtrate was dialyzed with a dialysis bag with a molecular weight cut-off of 1000D, and the dialysate was collected. Freeze-drying to obtain fluorescent carbon dot powder. Weigh an appropriate amount of fluorescent carbon dots, dissolve them in ultrapure water, and accu...

Embodiment 1

[0034] A method for preparing fluorescent carbon dots, comprising adding 3-aminoisonicotinic acid into ultrapure water for dispersion to obtain a 3-aminoisonicotinic acid ultrapure water dispersion with a mass-volume ratio of 0.1 mg / mL. The ultrapure water dispersion of 3-aminoisonicotinic acid was transferred into a polytetrafluoroethylene stainless steel autoclave, and placed in an oven for heating reaction. The reaction temperature was 120° C., and the reaction time was 12 hours. Centrifuge the reaction product and take the supernatant, filter the supernatant with a 0.22 μm microporous membrane, dialyze the obtained filtrate with a dialysis bag with a molecular weight cut-off of 1000D, collect the dialysate and freeze-dry it to obtain fluorescent carbon dots powder.

Embodiment 2

[0036] A method for preparing fluorescent carbon dots, comprising adding 3-aminoisonicotinic acid into ultrapure water for dispersion to obtain a 3-aminoisonicotinic acid ultrapure water dispersion with a mass-volume ratio of 5 mg / mL, and adding 3-aminoisonicotinic acid The nicotinic acid ultrapure water dispersion was transferred into a polytetrafluoroethylene stainless steel high-pressure reaction kettle, and placed in an oven for heating reaction. The reaction temperature was 160° C., and the reaction time was 10 h. Centrifuge the reaction product and take the supernatant, filter the supernatant with a 0.22 μm microporous membrane, dialyze the obtained filtrate with a dialysis bag with a molecular weight cut-off of 1000D, collect the dialysate, and finally freeze-dry to obtain fluorescent carbon dot powder .

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Abstract

The invention discloses a fluorescent carbon dot and its preparation method and application. 3-aminoisonicotinic acid is dispersed in ultrapure water to prepare an ultrapure water dispersion of 3-aminoisonicotinic acid. The ultrapure water dispersion of nicotinic acid is moved into a polytetrafluoroethylene stainless steel high-pressure reactor, placed in an oven to heat the reaction, the reaction liquid is centrifuged and the supernatant is collected, filtered with a microporous membrane, and the filtrate obtained is dialyzed. Finally, the dialysate is freeze-dried to obtain fluorescent carbon dot powder. The method has simple operation, low price and good repeatability; the prepared fluorescent spots have high light stability, low toxicity, good water solubility and good selectivity, and can detect and analyze tryptophan sensitively and specifically.

Description

technical field [0001] The invention belongs to the field of preparation of novel nanometer materials, and in particular relates to a fluorescent carbon dot and its preparation method and application. Background technique [0002] Tryptophan (Tryptophan, Trp) is the precursor of the important neurotransmitter 5-hydroxytryptamine in the human body and an important neurotransmitter. It is an essential amino acid for the human body and cannot be synthesized by itself. It can only be supplemented by diet or medication. The World Health Organization stipulates that the content of tryptophan in normal human body should not be less than 4mg kg -1 . More than 95% of tryptophan in the human body is decomposed by tryptophan 2,3-oxygenase in liver cells. When liver cells are damaged, the decrease in the number and activity of enzymes often leads to the disorder of tryptophan metabolism, which not only causes the accumulation of toxic substances in the nervous system, causes delusions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/15C09K11/65G01N21/64
CPCC01B32/15C09K11/65G01N21/6428G01N21/6486G01N2021/6432
Inventor 薛伟明李文帅黄赛朋周鑫温惠云
Owner NORTHWEST UNIV
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