Method for separating and measuring contents of various components in Huoxiang Zhengqi oral liquid by HPLC method

A technology of Zhengqi oral liquid and content, which is applied in the directions of measuring device, material separation, analysis of materials, etc., can solve problems such as insufficient description of the content of components in Huoxiang Zhengqi oral liquid, and achieves high accuracy, quality assurance, and simple method. Effect

Active Publication Date: 2019-09-13
CHONGQING TAIJI MEDICAL RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content recorded in the above technical manuals and published literature is not sufficient to explain the content of ingredients in Huoxiangzhengqi Oral Liquid

Method used

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  • Method for separating and measuring contents of various components in Huoxiang Zhengqi oral liquid by HPLC method
  • Method for separating and measuring contents of various components in Huoxiang Zhengqi oral liquid by HPLC method
  • Method for separating and measuring contents of various components in Huoxiang Zhengqi oral liquid by HPLC method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] 1. Reference substance stock solution

[0079] Accurately weigh the appropriate amount of glycyrrhizin coumarin, isoliquiritigenin, apiloliquiritin, naringerin rutin, liquiritin, and nobiletin, add chromatographic methanol and dissolve them to make 0.07450, 0.50002, 0.97600, 0.50010, 0.50000, 0.62700mg respectively / ml of the reference substance stock solution, stored in a refrigerator at 4°C for future use.

[0080] 2. Standard curve

[0081] Precisely draw the stock solution of glycyrrhizin coumarin reference substance under item 1), and dilute it successively to obtain 0.0075, 0.0037, 0.0019, 0.0009, 0.0006, and 0.0005 mg per 1 mL, to obtain 6 groups of reference substance solutions with different concentrations, numbered 1-6; Precisely draw the stock solution of isoliquiritigenin reference substance under item 1), and dilute it successively so that each 1mL contains 0.0250, 0.0125, 0.0063, 0.0031, 0.0016, 0.0008mg, and obtain 6 groups of reference substance solutio...

Embodiment 2

[0087] 1. Precision

[0088] Accurately measure the appropriate amount of 6 kinds of reference substance stock solutions, and dilute with methanol to obtain a mixed solution containing 0.002, 0.050, 0.005, 0.010, 0.050 and 0.003mg of the above 6 components per 1mL. Take 10 μL of the mixed solution, and measure it according to item "2.1" of Example 1. Six consecutive injections were made, and the peak area was recorded. Results The RSD values ​​of the peak areas of glycyrrhizin, isoliquiritigenin, apiloliquiritin, naringerin, liquiritin, and nobiletin were 1.06%, 0.17%, 1.43%, 1.24%, 0.23%, and 0.15%, respectively. , the RSD values ​​of the peak areas of the six components were all less than 3%, indicating that the precision of the instrument was good.

[0089] 2. Repeatability

[0090] Get 1 batch number and be the sample preparation need testing solution of 17080319, prepare sample by the method provided by the present invention, then dilute with methanol to obtain the mix...

Embodiment 3

[0096] Sample determination

[0097] Process 1 batch of samples according to the method of the present invention, measure according to embodiment 1 "2.1", adopt external standard method to calculate glycyrrhizin, isoliquiritigenin, apiloliquiritin, naringerin, liquiritin, Sichuan The content of dermoside was found to be 0.007%, 0.251%, 0.221%, 0.279%, 0.540% and 0.009%, respectively.

[0098] Table 2 Contents of 6 components in 10 batches of samples of Taiji Huoxiang Zhengqi Oral Liquid

[0099]

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Abstract

The invention belongs to the field of pharmaceutical preparations, relates to a quality control method of Huoxiang Zhengqi oral liquid, and in particular relates to a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. The invention provides a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. According to the HPLC method, a chromatographic column is adopted, and a silica gel matrix is taken as a filler, and a solution A and a solution B are mixed according to a certain proportion to be taken as a mobile phase for elution; the solution A is acetonitrile, and the solution B is phosphoric acid water; and the components comprise one or more of licocoumarin, isoliquiritigenin, apigenin liquiritin, naringin, liquiritin and nobiletin. The method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by the HPLC method is simple, sensitive and high in accuracy, can make up the defect that the prior art is not sufficient enough to explain the content of the components in the Huoxiang Zhengqi oral liquid, and effectively ensures the quality of the Huoxiang Zhengqi oral liquid.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a quality control method of Huoxiang Zhengqi Oral Liquid, in particular to a method for separating and measuring the contents of various components in Huoxiang Zhengqi Oral Liquid by HPLC. Background technique [0002] Huoxiang Zhengqi Oral Liquid is a Chinese patent medicine, which has the effects of relieving the exterior and dispelling dampness, regulating qi and harmonizing the middle. According to the first volume of the Chinese Pharmacopoeia, the prescription is composed of atractylodes atractylodes, Magnolia officinalis, Poria cocos, raw pinellia, patchouli oil, tangerine peel, Angelica dahurica, Dabupi, licorice extract and perilla leaf oil. The pharmacopoeia also records Magnolia officinalis , tangerine peel and perilla leaf oil by high performance liquid chromatography. In addition, in the existing literature, Yu Jiawen et al. established a UPLC analysis method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/047
Inventor 郭大乐邓赟秦少容陈金凤董自亮彭涛李张宇
Owner CHONGQING TAIJI MEDICAL RES INST CO LTD
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