Method for improving seed color sorting precision of genetically engineered genic male sterile line of rice by utilizing recessive glume shell color characters

Abstract

The invention discloses a method for improving the seed color sorting precision of a genetically engineered genic male sterile line of rice by utilizing recessive glume shell color characters. A recessive heterochromatic glume fertile rice material is bred into a two-color sortable genetically engineered genic male sterile line; a quaternary linkage vector containing a yellow glume gene, a fertility restoration gene, a pollen inactivation gene and a fluorescent marker gene is introduced into a genome of the two-color sortable genetically engineered genic male sterile line to breed a two-color sortable genetically engineered breeding line; the two-color sortable genetically engineered breeding line and the two-color sortable genetically engineered genic male sterile line are planted in a mixed mode, and all seeds are mixed and collected; fluorescent screening is carried out for the first time, and non-fluorescent sterile line seeds and fluorescent breeding line seeds are separated; and glume shell color screening is carried out for the second time, and the seeds of the heterochromatic glume shell sterile line are purified. According to the method, recessive heterochromatic glume shells of rice are used as new color sorting markers to improve the purity of the genetically engineered genic male sterile line seeds in the sorting process, and the objective is achieved that the purity of the genetically engineered genic male sterile line reaches the production application standard.

Description

technical field [0001] The invention relates to the technical field of rice genetic engineering, in particular to a method for improving the color selection accuracy of genetically engineered male sterile line seeds of rice by utilizing recessive glume color traits. Background technique [0002] The essence of the genetically engineered male sterile line is ordinary genetic male sterile rice, so it inherits the advantages of stable fertility, complete sterility and easy preparation of high-yielding, high-quality, multi-resistant hybrid combinations of ordinary genetic male sterile rice, which can avoid the current three The combination of two-line sterile lines is restricted by the strict recovery relationship and the two-line sterile line production is affected by temperature fluctuations. The third-generation hybrid rice breeding technology based on genetically engineered male sterile lines is currently the most ideal, convenient and efficient way to utilize heterosis. The...

AI Technical Summary

Problems solved by technology

However, in the process of using the existing fluorescence sorting technology to separate the sterile line seeds, because the rice endosperm is wrapped by the glume, the intensity of fluorescence emitted by the fluorescent protein from the endosperm to the outside of the glume is reduced, which interferes with the color sorting of the color sorter. Accuracy, the genetically engineered male sterile line selected often contains a few seeds of the breeding line with genetically modified components

Since the public is very sensitive to genetically modified products and the country has not released restrictions on genetically modified products, if a few seeds of the reproductive line carrying genetically modified ingredients are mixed with the seeds of the sterile line during the seed production process, it will result in the production of hybrid seeds containing A small number of seeds carry genetically modified ingredients, which seriously hinders the promotion and application of third-generation hybrid rice

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