Micro-fluidic chip and drug administration culture method for tumor cell three-dimensional culture

Abstract

The invention belongs to the field of micro-fluidic chips and particularly relates to a micro-fluidic chip and drug administration culture method for tumor cell three-dimensional culture. The micro-fluidic chip comprises a cell culture layer, a porous membrane layer and a cover glass layer which are sequentially stacked from top to bottom, wherein a downwardly concave cell culture area, a cell carrier liquid inlet channel and a cell carrier liquid outlet channel are arranged on the upper surface of the cell culture layer, the cell carrier liquid inlet channel and the cell carrier liquid outlet channel are respectively connected with two ends of the cell culture area, a plurality of downwardly concave cell culture cavities are arranged in the bottom surface of the cell culture area, and the porous membrane layer is provided with a porous area. The micro-fluidic chip is simple in structure, easy to process, capable of achieving high-throughput and uniform-size culture of tumor cell spheres, uniform drug or culture medium diffusion can be achieved, shear force to the tumor cell spheres is reduced, and the chip is applicable to in-vitro screening tests during anti-cancer drug development.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a microfluidic chip for three-dimensional culturing of tumor cells and a drug delivery and culturing method. Background technique [0002] With the rapid development of tissue engineering, a three-dimensional cell culture technology that can simulate the microenvironment of the human body has emerged as the times require, and gradually shows more comprehensive advantages than the traditional two-dimensional cell culture method. The application of three-dimensional cell culture technology to tumor research can explore a series of fields such as tumor invasion mechanism, tumor microenvironment, tumor drug screening, etc., and effectively solve the problem of low accuracy of two-dimensional culture experiment results. Microfluidic chip technology is widely used in cell research by virtue of its micro-scale, high efficiency and many other advantages. By setting up a reasonable cultu...

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Problems solved by technology

[0005] The invention provides a microfluidic chip for three-dimensional culture of tumor cells, which solves the problems that the tumor cell chip has a complex structure, is difficult to process, and is difficult to achieve high-throughput, uniform-sized tumor cell spheroid culture and uniform drug delivery.

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