Method for detecting bacterium resisting properties of electrical household appliances by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting the bacterium resisting properties of electrical household appliances. The method comprises the detection steps of preparing samples and performing pretreatment: performing type selection of equipment, and compounding a reagent and culture mediums; performing strain preservation, performing strain activation and preparing bacterium suspension; establishing a standard curve of the ATP concentration logarithm value lgC-relative fluorescence intensity logarithm value lgI, and performing calibration on the ATP concentration C of viable bacteria of inoculation bacterial liquid; performing sample inoculation, performing sample culture and performing eluting and recovering; determining I of recovery liquid, and reckoning ATP concentration C and ATP concentration T of the viable bacteria; calculating bacterium resisting rate R or bacterium resisting activity value A; and performing result evaluation. The method has the characteristic that the bacterium resisting properties which are represented by R or A, of the electrical home appliances can be accurately and quantitatively tested through an ATP fluorophotometer. Control samples and bacterium resisting samples after contact for 0h and culture for 24h are eluted and recovered according to the specification of the invention, the I of the recovery liquid is determined and represented by lgI, and R or A is calculated; and a result evaluation base is provided. According to the ATP biofluorescence lgC-lgI standard curve method for detecting the bacterium resisting properties of the electrical household appliances, researched and developed by the invention, product quality increase and market order standardization can be supported through an advanced detection technique.

Description

technical field [0001] The invention relates to an antibacterial performance test method of household appliances, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antibacterial performance of household appliances characterized by antibacterial rate R or antibacterial activity value A A -lgI A The standard curve method belongs to the technical field of antibacterial function detection of household appliances. Background technique [0002] Over the years, the innovation efficiency and market benefits of my country's home appliance industry cannot be underestimated. With the enhancement of people's awareness of health and environmental protection, consumers' antibacterial demand for home appliances is changing with each passing day. At the end of the last century, antibacterial home appliances came into being. The application and popularization of the industry is unstoppable, and the antibacterial fu...

AI Technical Summary

Problems solved by technology

However, the existing detection methods have the following common problems in technical content and practical application: first, the number of experimental bacteria involved is too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; second, the experimental process is cumbersome and related operations Affected by the professional experience of the experimenter, the test error is large and lacks comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic cost are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (Textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content characterizes the anti-microbial / fungal performance of the fluorescence assay, but it is only applicable to textile materials or microporous materials with water absorption and the growth value of the control sample / mycotic bacteria > 0. The key technical aspects are not applicable to plastics, stainless steel and other household appliance component materials with non-absorbent hard surfaces and the bacterial growth value of the control sample is <0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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