Establishment method and induced differentiation application of human marrow mesenchymal stem cell strain

Abstract

The invention discloses an establishment method and an induced differentiation application of a human marrow mesenchymal stem cell strain. A marrow mesenchymal stem cell extracted from a femoral shaft marrow cavity is high in purity and good in activity and can completely meet a series of experiments of osteogenesis induced differentiation of stem cells, an extracted marrow mesenchymal stem cell of an osteoporosis patient has expression difference in transcriptomics as compared with that of normal persons, and a new thought and method are provided for further disclose the osteoporosis pathogenesis of the marrow mesenchymal stem cell and seek a new treatment target. Besides, as for samples with fewer marrow mesenchymal stem cells of osteoporosis and the like, the technology can effectively increase the cell obtaining amount after extraction, increase the number of adherent cells, promotes the cells to rapidly enter a logarithmic growth stage, reduce the culture time and reduce the culture cost and more easily keep the dryness of the marrow mesenchymal stem cell, so that a series of experiment demands of osteogenic differentiation, stem cell treatment and the like are met.

Description

technical field [0001] The invention relates to the related field of treatment methods for orthopedic diseases, in particular to a method for establishing a human bone marrow mesenchymal stem cell line and an application for inducing differentiation. Background technique [0002] Primary osteoporosis is a systemic bone disease characterized by decreased bone mass and degeneration of bone tissue microstructure. With the increase of bone fragility, the risk of fracture increases. Current researches believe that the cause of osteoporosis is that the bone formation in bone tissue is significantly reduced and the bone resorption process is relatively enhanced. However, the reduction in the number of bone marrow mesenchymal stem cells and the weakening of their differentiation ability lead to the reduction of differentiation into osteoblasts and osteocytes, which weakens the osteogenic activity. Therefore, in the study of primary osteoporosis disease models, bone marrow mesenchyma...

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Problems solved by technology

At the same time, affected by factors such as oxidative stress, hormone level decline, and body function decline in the body, bone marrow mesenchymal stem cells show decreased activity, reduced differentiation function and relatively fragile, making the isolation and cultivation of bone marrow mesenchymal stem cells from patients with osteoporosis difficult. Mesenchymal stem cells are more difficult than normal

Therefore, the use of general separation methods is more likely to lead to cell rupture, apoptosis and necrosis, and there are situations such as low culture survival rate and cell purity that cannot meet the experimental requirements, which has caused problems for the research of osteoporosis.

However, for the existing methods and technologies, there is still a lack of a better extraction method and technology for bone marrow mesenchymal stem cells in patients with osteoporosis.

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