Method for evaluating bacteria killing effect of liquid disinfectant with ATP (adenosine triphosphate) bioluminescence lgCB-lgIB standard curve method

Abstract

The invention relates to a method for evaluating a bacterial vegetative form killing effect of a liquid disinfectant. The method comprises detection steps as follows: sample extraction and determination of the minimum effective concentration; type selection of equipment and preparation of reagents and culture media; strain preservation and activation and preparation of a bacterial suspension; establishment of an OD value-viable content logarithm value lgCB standard curve and calibration of an inoculant liquid CB; establishment of an lgCB-relative fluorescence intensity logarithm value lgIB standard curve; neutralizer identification and quantitative suspension test; IB determination of a recovery liquid and calculation of viable content CB and TB; calculation of kill ratio R or sterilization index A; result evaluation. The method is characterized in that an ATP fluorophotometer is applied to accurate and quantitative evaluation of the bacterial vegetative form killing effect of the R or A characterized liquid disinfectant. According to the method, test sample liquids and a control sample liquid after acting for different time periods are recovered, and IB is measured and R or A is characterized by lgIB and calculated; result evaluation basis is provided. According to the developed ATP bioluminescence lgCB-lgIB standard curve method for evaluating the bacterial vegetative form killing effect of the liquid disinfectant, the disinfection effect evaluation method system can be improved.

Description

Technical field [0001] The invention relates to a method for evaluating the killing effect of a liquid disinfectant on bacterial reproductive bodies, in particular to an ATP fluorophotometer to accurately kill the bacterial reproductive body of a liquid disinfectant characterized by killing rate R or sterilization index A Quantitative test of ATP biofluorescence lgC B -lgI B The standard curve method belongs to the technical field of disinfectant sterilization effect evaluation. Background technique [0002] With the improvement of people's living standards and health awareness, in order to cut off the transmission of infectious diseases, liquid disinfectants such as peroxides, aldehydes, phenols, and quaternary ammonium salts are used in medical, food, education, catering, home and other fields It is becoming more and more widespread, and its killing effect and broad-spectrum requirements for bacterial propagules, fungi and their spores and other pathogenic microorganisms are be...

AI Technical Summary

Problems solved by technology

Although my country has promulgated and implemented GB 15981-1995 "Evaluation Methods and Standards for Disinfection and Sterilization Effects", GB 15979-2002 "Hygienic Standards for Disposable Sanitary Products" and QB / T 2738-2012 "Antibacterial and Antibacterial Effects of Daily Chemical Products However, the existing detection methods have the following common problems in technical content and practical application: First, the number of experimental bacteria involved is too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; The second is that the experimental process is cumbersome, and the relevant operations are affected by the professional experience of the experimenter, the test error is large, and there is no comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic costs are high.

At present, the research on antibacterial and disinfection effect detection technology in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of detection results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of Textile products (textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content after inoculation of the sample is a fluorometric method to characterize the anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample bacteria / mold growth value > 0; liquid disinfectant Bacterial killing effect The evaluation must form a set of clear and specific methods for key technical aspects such as neutralization effect evaluation and result calculation formula, and also provide relevant measurement uncertainty evaluation

In addition, the antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the traditional killing rate R in my country is

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