Supercharge Your Innovation With Domain-Expert AI Agents!

A special pretreatment solution for rectal fluid samples and its preparation method

A pretreatment liquid and sample technology, applied in the fields of botanical equipment and methods, preparation of samples for testing, chemicals used for biological control, etc., can solve problems affecting the accuracy of test results, etc. Improve accuracy and ensure the effect of accuracy

Active Publication Date: 2021-09-24
强安医疗器械有限公司
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mold growth will seriously affect the accuracy of test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A special pretreatment solution for rectal fluid samples and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 illustrates high-concentration pretreatment liquid of the present invention

[0026] 1. Prepare 1000ml of 0.5mol / L, pH7.4 phosphate buffer solution;

[0027] 2. Add 3.2 g of disodium edetate and 0.6 g of sodium lauryl sulfate to the phosphate buffer solution obtained in step 1, and heat to dissolve in a water bath at 57°C;

[0028] 3. Add 7ml of isobutanol to the solution prepared in step 2, stir well to dissolve it completely;

[0029] 4. Add 80 g of shell iodine and 1.5 ml of methylisothiazolinone into the solution prepared in step 3, and stir to mix them evenly.

Embodiment 2

[0031] Embodiment 2 illustrates the preferred pretreatment liquid of the present invention

[0032] 1. Prepare 1000ml of 0.5mol / L, pH7.4 phosphate buffer solution;

[0033] 2. Add 2.0 g of disodium edetate and 0.4 g of sodium lauryl sulfate to the phosphate buffer solution obtained in step 1, and heat to dissolve in a 60°C water bath;

[0034] 3. Add 4ml of isobutanol to the solution prepared in step 2, stir well to dissolve it completely;

[0035] 4. Add 65 g of shell iodine and 0.1 ml of methylisothiazolinone into the solution prepared in step 3, and stir to mix them evenly.

Embodiment 3

[0037] Embodiment 3 illustrates the low concentration pretreatment liquid of the present invention

[0038] 1. Prepare 1000ml of 0.5mol / L, pH7.4 phosphate buffer solution;

[0039] 2. Add 1.6 g of disodium edetate and 0.2 g of sodium lauryl sulfate to the phosphate buffer solution obtained in step 1, and heat to dissolve in a water bath at 58°C;

[0040] 3. Add 3ml of isobutanol to the solution prepared in step 2, stir well to dissolve it completely;

[0041] 4. Add 55 g of shell iodine and 0.05 ml of methylisothiazolinone into the solution prepared in step 3, and stir to mix them evenly.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a special pretreatment solution for rectal fluid samples with the functions of mold inhibition and cell disruption. The components contained are methylisothiazolinone, sodium lauryl sulfate, disodium edetate, Butanol, chitosan‑iodine complex, and phosphate buffer. The present invention also relates to a method for preparing the above pretreatment liquid. The pretreatment solution of the present invention can provide a stable environment for the sample, because the combined application of methylisothiazolinone and shell iodine has the effect of disinfecting and inhibiting the growth of microorganisms, especially molds, in the pretreatment process of rectal fluid sample detection. At the same time, the triple crushing function of sodium lauryl sulfate, isobutanol and distilled water on the shaped cells is superimposed, so that the shaped cells in the sample are broken and released intracellular substances, preventing the solidification and precipitation of the shaped substances in the sample, ensuring that the sample detection accuracy.

Description

technical field [0001] The invention belongs to the field of medical supplies, in particular to a special pretreatment solution for rectal fluid samples and a preparation method thereof. Background technique [0002] Rectal fluid is a kind of human body sample used in medical clinical testing, which can be used for biochemical analysis and microbial detection of potassium, sodium, chlorine, pH value, heme protein, etc. Rectal fluid contains intestinal secretions, pathological exudates, residual food waste, shaped cells (red blood cells, white blood cells, plants, animals, microbial cells, etc.), and there may be residual feces, especially loose stools, etc. Intestinal contents. Due to the particularity of the environment in the rectum, there are many bacteria, molds, and parasites. The pre-treatment solution is a component of in vitro diagnostic reagents and requires a certain period of validity, generally 1 to 2 years. During the storage of liquid-based reagents, molds o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30A01N25/30A01N43/80A01N43/16A01N59/12A01P3/00A01P1/00
CPCA01N25/30A01N43/16A01N43/80A01N59/12G01N1/28G01N1/30
Inventor 张野杨新财
Owner 强安医疗器械有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com