Application of black raspberry anthocyanin in preparing anti-liver-injury drugs or health food

A health food, black raspberry technology, applied in the function, application, food ingredients and other directions of food ingredients to achieve the effect of reversing liver cell damage and reducing harm

Inactive Publication Date: 2019-10-01
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In the prior art, there is no relevant report on the treatment of liver cirrhosis, liver ascites, hepatitis, alcoholic liver injury, etc.

Method used

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  • Application of black raspberry anthocyanin in preparing anti-liver-injury drugs or health food
  • Application of black raspberry anthocyanin in preparing anti-liver-injury drugs or health food
  • Application of black raspberry anthocyanin in preparing anti-liver-injury drugs or health food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0028] Implementation Case 1: The preparation method of black raspberry extract

[0029] Accurately weigh 50 g of black raspberry freeze-dried powder, carry out ultrasonic extraction with 10 times the volume of 75% ethanol for 3 times, each time for 1 h, filter the extract with a Buchner funnel, combine the extract, and recover the solvent with a rotary evaporator to obtain Black raspberry extract with 75% ethanol.

Embodiment example 2

[0030] Implementation Case 2: The preparation method of the anthocyanin-rich part of black raspberry

[0031] Reconstitute the black raspberry 75% ethanol extract with 10% ethanol. After reconstitution, use polyamide to perform column chromatography, use 10% ethanol to remove impurities, and use 30% ethanol to elute, collect 30% ethanol to wash Take off the fraction, and carry out solvent recovery with a rotary evaporator to obtain an effective part rich in anthocyanins of black raspberry, wherein the main anthocyanins contained are cyanidin-3-O-glucoside (2.63mg / g), cyanidin Inulin-3-O-rutinoside (16.91 mg / g).

Embodiment example 3

[0032] Implementation Case 3: Experimental Study on Anti-hepatic Fibrosis Activity of Black Raspberry Anthocyanins

[0033] The anti-hepatic fibrosis activity of black raspberry-rich anthocyanins was tested by MTT method, and the effective fractions rich in anthocyanins (100, 75, 50, 25, 12.5 μg / mL) of black raspberry had the effect on human hepatic stellate cell HSC The results of inhibitory activity showed that the black raspberry extract had a good effect on inhibiting the growth of cancer cells at 25, 50, 75, and 100 μmol / L, and it was better than the positive drug tetrastreptomycin C, and the inhibitory effect was dose-dependent. The test results are shown in Table 1:

[0034] Table 1 Black raspberry extract has inhibitory activity on HSC cells

[0035]

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PUM

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Abstract

The invention belongs to the technical field of medicine, and discloses application of black raspberry anthocyanin in preparing anti-liver-injury drugs. The black raspberry anthocyanin is an anthocyanin component rich in a black raspberry extract, and has the effect on treating liver injuries, the effective dose of the black raspberry anthocyanin is 25-100 mg / kg / d. In vivo studies in mice show that the black raspberry anthocyanin can reduce the harm of chemical liver injuries, and significantly reduce the levels of AST, ALT, CHOL, LDL and TBIL in mouse serum, and liver pathological sections ofthe mice show that the black raspberry anthocyanin can significantly reverse the trend of the liver injuries. Therefore, the black raspberry anthocyanin can be well applied to the anti-liver-injury drugs and health food.

Description

technical field [0001] The invention mainly belongs to the field of natural product chemistry, and mainly relates to the application of black raspberry anthocyanins in the preparation of anti-liver damage medicines or health food, and specifically relates to black raspberry (including black raspberry fruit and black raspberry leaves) rich in flower and color The application of glycosides in the preparation of anti-liver damage drugs and health food. Background technique [0002] The liver is an organ in the body that mainly has metabolic functions, and plays a role in the body such as deoxidation, storage of glycogen, and synthesis of secreted proteins. The liver is the largest organ in the human body and the largest digestive gland in the human digestive system. [0003] According to the cause of the disease, liver injury is mainly divided into three types: viral, chemical and alcoholic. Liver disease directly affects people's life and health, and even threatens people's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P1/16A23L33/105A61K131/00
CPCA23V2002/00A61K36/73A61K2236/333A61K2236/55A23L33/105A61P1/16A23V2200/32
Inventor 赵余庆肖婷
Owner SHENYANG PHARMA UNIVERSITY
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