Stenotrophomonas for efficiently degrading poly(butylene adipate-co-terephthalate) and application thereof

Abstract

The invention provides stenotrophomonas for efficiently degrading poly(butylene adipate-co-terephthalate) and application thereof. A strain of poly(butylene adipate-co-terephthalate) (PBAT) degradingbacteria capable of secreting lipase is separated and screened out of the soil in Yanxia town, Liquan county, Shanxi province, and the stenotrophomonas is named as Stenotrophomonas sp.strain YCJ1, ispreserved in China Center For Type Culture Collection and has the preservation number of CGMCC 18059. The stenotrophomonas has the advantages that the stenotrophomonas can secret lipase and efficiently degrade PBAT, the stenotrophomonas has a more excellent degrading effect under the slightly alkaline conditions, and the stenotrophomonas and the lipase thereof can be applied as enzymic preparations, biological bactericides and biological enhancers to the environment repairing of PBAT and PBAT-base biodegradable materials in the slightly alkaline environment and have high application value.

Description

Technical field [0001] The invention belongs to the technical field of PBAT degradation, and specifically relates to a strain of Stenotrophomonas sp. strain YCJ1 that efficiently degrades PBAT. Background technique [0002] Polybutylene terephthalate / adipate (PBAT) is an aliphatic biodegradable material. Due to the good mechanical properties, thermoplasticity and usability of traditional plastics, PBAT can be protected by microorganisms in the environment. It is degraded by lipase and has good biodegradability, making PBAT widely used in agriculture, food and packaging industries. However, current studies have found that the degradation degree of PBAT is mainly closely related to the physical and chemical properties of the environment such as pH, temperature and humidity. This is reflected in the degradation rate of PBAT in the northwest region is much lower than that in the southwest region, and the type and quantity of microorganisms in the environment in the northwest region ...

AI Technical Summary

Problems solved by technology

However, current studies have found that the degree of degradation of PBAT is closely related to the physical and chemical properties of the environment, such as pH, temperature, and humidity. This is reflected in the fact that the degradation rate of PBAT in the northwest region is far lower than that in the southwest region. Moreover, the types and quantities of microorganisms in the environment in the northwest region Less, which makes the application of PBAT severely limited

Therefore, there is an urgent need for an environment-tolerant PBAT-degrading bacteria to solve the problem of low degradation rate of PBAT in different regions.

[0003] At present, there are few studies on PBAT-degrading bacteria. The PBAT-degrading bacteria reported at home and abroad mainly include Sphingopyxis ginsengisoli, Bacillus pumilus, Pseudomonas genus Pseudomonas pseudoalcaligenes) and the fungus Trichoderma asperellum (Trichoderma asperellum), but there is generally a problem of low degradation rate, and the degradation of PBAT by strains is generally slow in an alkaline environment

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