Long-chain RNA Lnc-13814 for regulating and controlling follicle development of laying ducks and application thereof

A technology of rnalnc-13814 and follicle development, applied in the field of genetic engineering, to achieve the effect of improving the fecundity of laying ducks
CN110295170AActive Publication Date: 2019-10-01INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
Publication Date
2019-10-01

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Abstract

The invention provides a long-chain RNA Lnc-13814 for regulating and controlling follicle development of laying ducks and its application. A cDNA sequence corresponding to the long-chain RNA Lnc-13814related to follicular development of laying ducks is shown as SEQ ID NO:1. The invention also provides a fluorescent quantitative PCR detection primer pair of the long-chain RNA Lnc-13814 and detection kit. The detection primer pair is nucleotide sequences shown as SEQ ID NO:2 and SEQ ID NO:3. The invention also provides an over-expression vector of the long-chain RNA Lnc-13814 and a constructionmethod thereof. It is found for the first time that the long-chain RNA Lnc-13814 has the function of promoting the proliferation of follicular cells of laying ducks and has the important significancein study of the genetic nature of reproductive traits of laying ducks and improvement of the reproductive capacity of the laying ducks.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to a long-chain RNALnc-13814 regulating egg follicle development of laying ducks and its application. Background technique

[0002] Follicle development is closely related to the laying performance of laying ducks and directly affects the egg production of laying ducks. Studies have shown that poultry follicle development is an extremely complex biological process. At present, people have a certain understanding of the follicle development model of poultry. However, as an important factor that determines egg production, the specific regulatory mechanism of follicle development still needs to be further studied. Research. The growth of granulosa cells directly affects the development of follicles. Follicular granulosa cells can secrete gonadal hormones and growth factors to regulate the growth, differentiation and maturation of follicular theca cells and oocytes, and als...

Claims

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