Unlock instant, AI-driven research and patent intelligence for your innovation.

Dihydroagarwood furan-type sesquiterpenes and their preparation and application

A kind of dihydro agarwood and furan type technology, applied in the field of medicine, can solve the problems such as patents or literature reports that have not yet been seen

Active Publication Date: 2022-04-05
SHENYANG PHARMA UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The compounds involved in the present invention and their activity have not been patented or reported in the literature so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dihydroagarwood furan-type sesquiterpenes and their preparation and application
  • Dihydroagarwood furan-type sesquiterpenes and their preparation and application
  • Dihydroagarwood furan-type sesquiterpenes and their preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: the preparation of formula compound 1-15

[0066] Take 30 kg of dried Tripterygium wilfordii leaves and extract them three times with ethanol with a volume concentration of 75%. The extraction adopts heating reflux extraction or ultrasonic extraction. Combine all the extracts, concentrate under reduced pressure, recover the solvent, and dry to obtain the extract after completing the concentration under reduced pressure. Sequentially extracted with ethyl acetate and n-butanol, the ethyl acetate layer was subjected to gel column chromatography, petroleum ether-EtOAc mixed system (100:1, 30:1, 15:1 and 10:1, 1:1, v / v ) was eluted to obtain fractions A-D, and fraction C was eluted through cross-linked dextran LH-20 column and ODS gel column to obtain 5 main fractions C1-C5, and C2 (9.5g) was chromatographically separated on a silica gel column , eluted with a petroleum ether-ethyl acetate gradient (25:1 to 1:1) to obtain 8 subfractions (Fr.C2-1 to Fr.C2-8). ...

Embodiment 2

[0067] Example 2: Anti-inflammatory activity of compounds against lipopolysaccharide induction

[0068] 1. Experimental materials

[0069] 1.1 Experimental reagents: Compound 1-15 mouse mononuclear macrophage RAW 264.7 (ATCCTib-71) was purchased from the Chinese Academy of Sciences. RPMI 1640 medium, penicillin, streptomycin and fetal bovine serum were purchased from Invitrogen, USA. Lipopolysaccharide (LPS), dimethylsulfoxide (DMSO) and hydrocortisone were purchased from Sigma.

[0070] 2 test process

[0071] 2.1 Cell culture and treatment

[0072] RAW 264.7 cells were cultured in RPMI 1640 medium containing penicillin (100U / ml), streptomycin (100g / ml) and 10% heat-inactivated fetal bovine serum (5% CO2 and 95% air), every two days on average Change medium once. 264.7 cells were trypsinized to confluence and assayed during exponential growth. Compounds 1-15 were dissolved in dimethyl sulfoxide (DMSO) at the cell culture level and added to the culture medium to obtain a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicine, relates to dihydroagarwood furan-type sesquiterpenes and its preparation and application, and relates to a preparation method of dihydroagarwood furan-type sesquiterpenoids from the dry leaves of tripterygium genus Tripterygium genus Euonymus genus tripterygium , the present invention also relates to the application of this type of compound in the preparation of anti-inflammatory drugs. The structure of the described dihydroagarwood furan-type sesquiterpenes is as follows: the present invention passes through the leaves of Tripterygium wilfordii through silica gel column chromatography, gel column chromatography, HP‑20 macroporous resin, open ODS column chromatography, and high performance liquid phase The compound is isolated and prepared, and the prepared compound has obvious anti-inflammatory activity.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, relates to dihydroagarwood furan-type sesquiterpenes and its preparation and application, and relates to a preparation method of dihydroagarwood furan-type sesquiterpenoids from the dry leaves of tripterygium genus Tripterygium genus Euonymus genus tripterygium , the present invention also relates to the application of this type of compound in the preparation of anti-inflammatory drugs. Background technique: [0002] Tripterygium wilfordii (Tripterygium wilfordii HooK.f.) belongs to the genus Tripterygium wilfordii HooK.f., a perennial vine shrub, mainly distributed in the south of the Yangtze River Basin and the southwest region. It has various physiological activities such as anti-inflammation, analgesia, anti-tumor, and immune regulation, and is often used to treat diseases such as rheumatoid arthritis, skin eczema, and malignant tumors. At present, the research on the chemical constituents...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/00A61K31/343A61P29/00
CPCC07D307/00A61P29/00C07B2200/13
Inventor 宋少江黄肖霄卢丽威周乐何青军
Owner SHENYANG PHARMA UNIVERSITY