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Human MOG antigen epitope peptide, antigen, antibody, application and chemiluminescence kit

A technology of chemiluminescent reagents and antigen epitopes, applied in chemiluminescence/bioluminescence, analysis by making materials react chemically, peptides, etc., can solve the problem of lack of auxiliary effects, inability to activate B cells, unclear pathogenic mechanism, etc. problems, achieve the effects of enhancing luminous intensity and time, improving detection sensitivity and result reliability, and quickly and timely diagnosing the disease

Active Publication Date: 2019-10-11
深圳市安群生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the exact pathogenic mechanism of MOG-Ab disease is still unclear. Most studies believe that specific B cells that recognize MOG antigens may exist in peripheral blood, but due to the lack of expression of MOG antigens in bone marrow, MOG-specific immature B cells The cells are in an anergic state, and at the same time, due to the lack of corresponding helper T cells (Th cells), the specific B cells that recognize MOG cannot be activated, but only proliferate in the peripheral blood

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  • Human MOG antigen epitope peptide, antigen, antibody, application and chemiluminescence kit
  • Human MOG antigen epitope peptide, antigen, antibody, application and chemiluminescence kit
  • Human MOG antigen epitope peptide, antigen, antibody, application and chemiluminescence kit

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preparation example Construction

[0058] The preparation method of the MOG epitope peptide of the present invention can be a chemical synthesis method: the antigen epitope peptide is synthesized by a solid-phase method using an American ABI431A type polypeptide automatic synthesizer. The molecular weights of the antigenic epitope peptides (1) and (2) of the present invention are 1639.78 and 2051.18 respectively, which can be determined by mass spectrometry, and the synthesized antigenic epitope peptide sequences are identified by polypeptide sequence determination. The purity of the peptide can be assessed by thin-layer chromatography and high-performance liquid chromatography, and the concentration of the epitope peptide can be determined.

[0059] 2. MOG-coated antigen, MOG-bound antigen

[0060] The invention also provides the MBP antigen, which can be used as the coating antigen and binding antigen in the double antigen sandwich method, so as to be used to prepare the human MOG-Ab in vitro diagnostic kit. ...

Embodiment 1

[0112] Example 1: Preparation of MOG epitope peptides (1) and (2).

[0113] The preparation method uses chemical synthesis method: the MOG antigenic epitope peptides (1) and (2) are synthesized respectively by solid-phase method using the American ABI431A automatic peptide synthesizer. The purity of the epitope peptide was assessed by high performance liquid chromatography, and the concentration of the peptide was determined. The molecular weights of the antigenic epitope peptides (1) and (2) of the present invention are 1639.78 and 2051.18 respectively, which are determined by mass spectrometry, and the synthesized polypeptide sequences are identified by polypeptide sequence determination.

[0114] 1. Synthesis of MOG epitope peptides (1) and (2)

[0115]The above peptides were synthesized by solid-phase method. The main idea of ​​solid-phase peptide synthesis is: first connect the carboxyl group of the carboxyl-terminal amino acid of the peptide chain to be synthesized wit...

Embodiment 2

[0202] Example 2: The MOG antigen epitope peptides (1) and (2) obtained in Example 1 were linked with carrier protein to prepare MOG antigens (1) and (2), respectively, using the obtained antigens (1) and (2) Animals are immunized to prepare specific monoclonal and polyclonal antibodies using the antigen (1), and specific monoclonal and polyclonal antibodies are prepared using the antigen (2).

[0203] 1. Preparation of antigen: MOG peptides (1) and (2) were respectively connected with carrier protein KLH (keyhole limpet hemocyanin) (purchased from sigma company) by BDB (Bis-diazotizedbenzidine dichloride) method to prepare MOG antigen ( 1) and (2).

[0204] Take 8.0mg of MOG peptide (1) or (2), dissolve it with 1ml of 0.1M PBS buffer (pH 7.4); dissolve 10mg of KLH with 20ml of 0.2M borate buffer (pH 8.6); then mix the two , cooled to 0°C, take BDBCl 2 110 μL, reacted at room temperature for 1.5 h, dialyzed overnight, then aliquoted, and stored at -20 °C.

[0205] In each ...

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Abstract

The invention relates to a human MOG antigen epitope peptide, an antigen, an antibody, application, an in-vitro diagnosis kit and a chemiluminescence kit for detecting human MOG-Ab. The amino acid sequence of the human MOG antigen epitope peptide is one of the sequences shown as SEQ ID NO.1 and SEQ ID NO.2. The chemiluminescence kit for detecting the human MOG-Ab adopts a double-antigen sandwich method to detect the human MOG-Ab and contains a chemiluminescent substrate. The human MOG antigen epitope peptide has good antigenicity and can generate highly specific monoclonal antibodies and polyclonal antibodies, and the chemiluminescence kit has the advantages of being high in sensitivity, strong in specificity, low in price, stable, long in validity period, stable and rapid in method, widein detection range, simple in operation, high in automatic degree and the like.

Description

technical field [0001] The invention belongs to the field of polypeptide chemistry and immunology, and in particular relates to a human anti-myelin oligodendrocyte glycoprotein (MOG) epitope peptide, a MOG specific antigen prepared by using the epitope peptide, and a corresponding monoclonal antibody or Polyclonal antibody, application of said antigen in preparation of human MOG-Ab in vitro detection kit, human MOG-Ab in vitro diagnostic kit, and a chemiluminescence kit for quantitatively detecting human MOG-Ab in a sample and its Preparation. Background technique [0002] At present, the exact pathogenic mechanism of MOG-Ab disease is still unclear. Most studies believe that specific B cells that recognize MOG antigens may exist in peripheral blood, but due to the lack of expression of MOG antigens in bone marrow, MOG-specific immature B cells The cells are in an anergic state, and at the same time, due to the lack of corresponding helper T cells (Th cells), the specific B...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K16/18G01N21/76G01N33/68
CPCC07K7/08C07K16/18G01N33/6893G01N33/6854G01N21/76
Inventor 朱建安朱仕杰
Owner 深圳市安群生物工程有限公司
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