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Method for detecting a2β-casein content in milk

A technology for casein and cow's milk is applied in the field of detecting A2β-casein content in cow's milk, which can solve the problems that need to be studied and cannot realize quantitative detection of A2β-casein.

Active Publication Date: 2021-11-09
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some patents disclose detection methods for β-casein, such as "A Method for Detection of β-Casein Content in Milk" with application number 200810146950.X and "Detection of A1β-casein and A2β-casein method”, but these international standards and patents only measure the content of total protein and total casein in milk or qualitatively detect A2β-casein in milk, and cannot realize the detection of A2β-casein in milk. Quantitative detection
[0005] Therefore, the method for quantitative detection of A2β-casein needs to be studied

Method used

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  • Method for detecting a2β-casein content in milk
  • Method for detecting a2β-casein content in milk
  • Method for detecting a2β-casein content in milk

Examples

Experimental program
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Effect test

Embodiment 1

[0075] Utilize the method for detecting the A2β-casein content in milk of the embodiment of the present invention to detect the A2β-casein content in raw milk, the specific method is as follows:

[0076] 1. Preparation of sample buffer solution:

[0077] Prepare 100ml sample buffer solution with deionized water, so that the concentration of each component is as follows: the concentration of sodium dihydrogen phosphate is 10mmol / L, trishydroxymethylaminomethane (TRIS) is 120mmol / L, urea (UREA) is 8mol / L, Disodium ethylenediaminetetraacetic acid (EDTA-2Na) is 45mmol / L, dithiothreitol (DTT) is 0.1%, and the pH value is adjusted to 8.0 with 0.1mol / L NaOH, and prepared with a 0.45μm water filter membrane The solution was filtered, sonicated, and set aside.

[0078] 2. Preparation of electrophoresis buffer solution:

[0079] Accurately prepare 100ml buffer solution with deionized water, so that the concentration of each component is as follows: phosphoric acid 0.15mol / L, sodium di...

Embodiment 2

[0095] Embodiment 2: Detect the content of A2β-casein in raw milk

[0096] Utilize the method for detecting the A2β-casein content in milk of the embodiment of the present invention to detect the A2β-casein content in raw milk, the specific method is as follows:

[0097] 1 Preparation of sample buffer solution:

[0098] Prepare 100ml sample buffer with deionized water, so that the concentration of each component is as follows: the concentration of sodium dihydrogen phosphate is 5mmol / L, trishydroxymethylaminomethane (TRIS) 100mmol / L, urea (UREA) 6mol / L, ethylene dihydrogen phosphate Disodium amine tetraacetate (EDTA-2Na) 30mmol / L, dithiothreitol (DTT) 0.15%, adjust the pH value to 7.5 with 0.1mol / L NaOH, and filter the prepared solution with a 0.45 μm water filter membrane, Ultrasound, back up.

[0099] 2 Preparation of electrophoresis buffer solution:

[0100] Accurately prepare 100ml buffer solution with deionized water, so that the concentration of each component is as f...

Embodiment 3

[0115] Utilize the method for detecting the A2β-casein content in milk of the embodiment of the present invention to detect the A2β-casein content in raw milk, the specific method is as follows:

[0116] 1. Preparation of sample buffer solution:

[0117] Prepare 100ml sample buffer solution with deionized water, so that the concentration of each component is as follows: the concentration of sodium dihydrogen phosphate is 15mmol / L, trishydroxymethylaminomethane (TRIS) 150mmol / L, urea (UREA) 10mol / L, ethylene dihydrogen phosphate Disodium amine tetraacetate (EDTA-2Na) 50mmol / L, dithiothreitol (DTT) 0.05%, adjust the pH value to 8.6 with 0.1mol / L NaOH, and filter the prepared solution with a 0.45μm water filter membrane , ultrasound, spare.

[0118] 2 Preparation of electrophoresis buffer solution:

[0119] Accurately prepare 100ml buffer solution with deionized water, so that the concentration of each component is as follows: phosphoric acid 0.15mol / L, sodium dihydrogen phosph...

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Abstract

The invention discloses a method for detecting A2β-casein content in milk. The method comprises: cooling and centrifuging milk to obtain a stock solution to be tested; diluting the stock solution to be tested with a sample diluent to obtain a diluted stock solution, wherein the sample diluent contains 5 ‑15mmol / L sodium dihydrogen phosphate concentration, 100‑150mmol / L tris, 6‑10mol / L urea, 30‑50mmol / L disodium edetate and 0.05‑0.15% dithiothreose Alcohol, and the pH value of the sample diluent is 7.5-8.6; and the diluted stock solution is detected by capillary electrophoresis, so as to determine the A2β-casein content in the milk. The method can quantitatively detect A2β-casein, and has good specificity, high accuracy, short detection time, simple operation and good repeatability.

Description

technical field [0001] The invention relates to the field of food, in particular to a method for detecting the content of A2β-casein in milk. Background technique [0002] The milk protein in milk mainly includes casein and whey protein. Casein accounts for about 80% of milk protein, mainly including α-casein, β-casein and κ-casein. Among them, β-casein accounts for 25%-30% of the total protein and is an important source of amino acids. The polymorphism of β-casein is relatively complex. There are 13 genetic variants detected so far, including A1, A2, A3, A4, B, C, and D. Their difference is one or more of the 209 amino acids that make up the protein. Types A1 and A2 beta-casein are the two most common in dairy herds. The difference is that there is a base change in the β-casein gene, which leads to a change in the amino acid at the corresponding position. The 67th position of the amino acid chain of A1β-casein is histidine (Histidine), and the 67th position of the amino ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N1/28G01N1/42G01N1/38G01N1/44
CPCG01N1/28G01N1/38G01N1/42G01N1/44G01N27/44721G01N27/44791
Inventor 李周勇刘宇陈云
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD