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Bioactive peptides with apn inhibitory effect

A technology of bioactive peptides and inhibition, which is applied in the direction of peptides, drug combinations, allergic diseases, etc., can solve the problems of high price, long research cycle of separation and purification of bioactive peptides, etc., to achieve good immunity activity of the body, promote anti-inflammatory Effect of tumor function, ability to improve

Active Publication Date: 2021-03-26
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the isolation and purification of bioactive peptides is a long and expensive research cycle, so virtual screening methods can be used to advance this process

Method used

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  • Bioactive peptides with apn inhibitory effect
  • Bioactive peptides with apn inhibitory effect
  • Bioactive peptides with apn inhibitory effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Screening of Active Peptides with APN Inhibition

[0026] Virtual enzymolysis of egg proteins (ovomucin, avidin, ovotransferrin) by pepsin and trypsin using the online tool ExPASy PeptideCutter (http: / / web.expasy.org / peptide_cutter / ) The peptide sequences generated after the solution were compared with the known APN inhibitory peptides in the BIOPEP (http: / / www.uwm.edu.pl / biochemia / index.php / en / biopep) database, and three unreported peptides were screened. Peptide CDR, SDF, ADF, FYQ, GEF, NTF, LWK, MIR, NAF, AQF, CNR. Water solubility, biological activity and ADMET (absorption, distribution , metabolism, excretion, toxicity) properties prediction. The screened tripeptides CDR, SDF, GEF, ADF, LWK, and CNR have good solubility, activity score higher than 0.5, and non-toxicity.

[0027]The crystal structure (4FYR) of APN was obtained from the PDB database (http: / / www.rcsb.org / ), and it was used as a protein target, and the tight binding ability of the peptide...

Embodiment 2

[0028] Example 2. Identification of APN inhibitory activity in vitro

[0029] The APN inhibitory activities of peptides CNR, CNR and GEF were determined by microplate reader. Take 20 μL of active peptide, add 20 μL of a standard product with a concentration of 60 U / L and mix well, preheat in a 37°C constant temperature water bath for 10 minutes, and then take 20 μL of the mixture. Add 30 μL of sample diluent to the enzyme-labeled plate, mix well with the mixture, add 100 μL of enzyme-labeled reagent, seal the plate with a sealing film, and incubate at 37°C for 60 minutes. Dilute the concentrated washing solution 20 times with distilled water before use. Peel off the sealing film, discard the liquid, shake dry, fill each well with washing solution, let it stand for 30 seconds and then discard, repeat this 5 times, and pat dry. First add 50 μL of chromogenic reagent A to each well, then add 50 μL of chromogenic reagent B, shake and mix well, and develop color at 37°C in the da...

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Abstract

The invention discloses bioactive peptides with APN inhibitory effect, the amino acid sequences of which are respectively Cys-Asn-Arg, Cys-Asp-Arg and Gly-Glu-Phe. The invention proves that the bioactive peptides Cys-Asn-Arg, Cys-Asp-Arg and Gly-Glu-Phe have APN inhibitory effect and anti-tumor potential activity through structure screening and activity measurement. At the same time, the bioactive peptides Cys‑Asn‑Arg, Cys‑Asp‑Arg and Gly‑Glu‑Phe exist stably under in vivo digestion conditions, can be fully absorbed and utilized by the body to exert their biological activity, and have anti-tumor functions for development Food, health products and medicines are of great significance.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a bioactive peptide with APN inhibitory effect. Background technique [0002] Bioactive peptides, as bioactive ingredients that promote health, can effectively promote the function of the body or improve the state of the body, and have the functions of transmitting physiological information and regulating physiological functions. Bioactive peptides are often used as functional ingredients in food production, especially in food and health products, and have achieved good results. Eggs have long been considered an ideal source of bioactive peptides. The bioactive peptides in eggs not only meet the basic nutritional needs, but also play a variety of different biological functions. Regulators have the functions of regulating immune response, anti-tumor, etc., and can scavenge free radicals and have the effect of delaying aging. [0003] Egg protein is decomposed and utilized by pepsin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083A61P35/00A61P37/04
CPCA61P35/00A61P37/04C07K5/0806C07K5/081
Inventor 赵文竹张丹于志鹏励建荣
Owner BOHAI UNIV
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