Nucleic acid set, kit and detection method for detecting Hantaan viruses through RPA (recombinase polymerase amplification)

A technology of Hantaan virus and a kit, applied in the biological field, can solve the problems of rapid detection of Hantaan virus, inability to separate from price automatic instruments, failure to perform typing, etc., and achieves short detection time, short response time, and simplicity. carry effect
CN110373502AActive Publication Date: 2019-10-25中国人民解放军东部战区疾病预防控制中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中国人民解放军东部战区疾病预防控制中心
Publication Date
2019-10-25

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Abstract

The invention relates to a nucleic acid set, a kit and a detection method for detecting Hantaan viruses through RPA (recombinase polymerase amplification). The nucleic acid set comprises a primer pairand a probe. The primer pair comprises a forward primer and a reverse primer, the sequence of the forward primer is SEQ ID NO. 2, the sequence of the reverse primer is SEQ ID NO. 3, and the sequenceof the probe is SEQ ID NO. 4. The kit comprises the nucleic acid set, and is applied to the detection method. Amplification can be realized at a temperature near to the body temperature, and visual discrimination of amplified products can be realized by a disposable nucleic acid detecting device. The nucleic acid set, the kit and the detection method have the advantages of high sensitivity and specificity, low requirement on hardware equipment, short reaction time, no complicated treatment on samples, suitability for on-site detection and the like, and are suitable for popularization and application.
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Description

technical field

[0001] The invention relates to a set of nucleic acid, a kit and a detection method for detecting Hantaan virus with RPA. The set of nucleic acid includes primers and probes, and belongs to the field of biotechnology. Background technique

[0002] Hemorrhagic fever with renal syndrome (HFRS) is a natural source of disease caused by Hantavirus (HV), characterized by high fever, hemorrhage, hypotension, proteinuria and other renal dysfunction. Rats are the main source of infection. Inhalation of virus-contaminated aerosols, direct contact of skin or wounds with excrement and secretions of host animals, and eating food contaminated by host animal excreta can cause infection. There are about 60,000 to 100,000 cases of HFRS in the world every year, mainly in Eurasia, and the mortality rate is 3% to 10%. my country is the main epidemic area of ​​HFRS in the world, and the number of cases is about 50,000 to 80,000 per year, accounting for more than 80% of the world...

Claims

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