Detection kit of group B streptococcus rapid serological typing and preparation method thereof

Abstract

A detection kit of group B streptococcus rapid serological typing and a preparation method thereof relate to microbiological detection. The detection kit of group B streptococcus rapid serological typing is provided with a kit body and test paper arranged in the kit body. The test paper is provided with a bottom lining, a sample pad, a marking pad, a coating film, and filter paper, wherein the sample pad, the marking pad, the coating film, and the filter paper are successively connected from the left to the right and are arranged on the bottom lining. The method comprises 1) preprocessing fluorescent particles; 2) activating fluorescent microspheres; 3) combining group B streptococcal antibodies; 4) preparing the marking pad; 5) preparing coating antibodies; and 6) assembling strips. The kit and the method are convenient and fast. When the kit and the method are applied to sample detection, people only needs to wait 10 to 15 minutes after the sample is added and then a detection resultcan be acquired. Operation is simple. The kit and the method can be used for group B streptococcus pure bacteria colonies of clinical culture identification, an enriched culture solution and detection and typing of secretion specimens for clinical routine sampling.

Description

technical field [0001] The invention relates to microorganism detection, in particular to a detection kit for rapid serological typing of group B streptococci and a preparation method thereof. Background technique [0002] Group B Streptococcus (group B streptococcal, GBS), also known as Streptococcus agalactiae, is an opportunistic pathogen that resides in the urogenital tract and lower gastrointestinal tract. It has been reported in the literature that pregnant women infected with GBS may cause adverse pregnancy outcomes such as premature delivery, premature rupture of membranes, intra-amniotic infection, and puerperal infection ([1] Landwehr-Kenzel, S. and P. Henneke, Interaction of Streptococcus agalactiae and Cellular Innate Immunity in Colonization and Disease. Front Immunol, 2014.5: p.519); GBS infection in newborns can cause neonatal pneumonia, sepsis, meningitis, toxic shock, and severe cases may be at risk of death ([2] Nielsen, M ., N. Sheikh, E. Fitzgerald, et a...

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Problems solved by technology

The traditional method of separating a single colony on a blood plate for bacterial culture identification is more complicated to operate, has many human interference factors, and has a low positive rate ([4] Shi Chunyan, Qu Shouhui, Yang Lei, etc., Group B Streptococcus carrier status of pregnant women in late pregnancy The impact of detection and carrier on pregnancy outcomes[J]. Chinese Journal of Obstetrics and Gynecology, 2010, 45(1) 12-16); in molecular biology detection methods, PCR detection technology is commonly used, which has strong specificity and high sensitivity , can be operated in batches. Compared with the conventional microbial culture method, the positive detection rate is greatly improved. However, the PCR method has high requirements for the laboratory, requiring a proprietary PCR laboratory and special experimental equipment. It is impossible to obtain GBS strains and conduct drug sensitivity tests. Other shortcomings ([5] Jia Zhonglan, Lu Xin, Xu Lifeng. Effect evaluation of three methods for screening group B streptococcus in pregnant women [J] Marker Immunoassay and Clinic, 2017, 24(3): 338-339)

Compared with the previous two methods, immunochromatography is simple to operate and takes a short time to produce results. In China, colloidal gold immunochromatography is used for rapid antigen detection of GBS, but there is currently no method for directly detecting its serotype, and for The clinical detection of GBS can quickly identify its serotype, which is more meaningful for preventive medicine

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