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A method for preparing yeast culture based on distiller's grains and its application in animal feed

A yeast culture and animal feed technology, applied in animal feed, animal feed, applications, etc., can solve the problems of not getting a higher crude fiber degradation rate, lower nitrogen-free extract content, and low crude fiber degradation rate. Achieve the effect of increasing the content and proportion of absorbable protein, reducing additives, and increasing nutritional value

Active Publication Date: 2021-04-06
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Distiller's grains are the residues of sorghum, corn, wheat and other single raw materials or mixed raw materials after fermentation and distillation. They are feed raw materials with high nutritional value. Compared with raw materials, the content of crude protein and crude fat in distiller's grains has increased, the content of nitrogen-free extracts has decreased correspondingly, and the content of B vitamins is also higher. It can be added to the diet of livestock and poultry as roughage, but white distiller's grains Distiller's grains also contain a large amount of crude fiber, which mainly comes from the rice husk used as a filler in the liquor brewing process. These crude fibers include lignin, cellulose, and hemicellulose, which cannot be digested by monogastric animals and have poor palatability. To improve the feeding value of distiller's grains, in the prior art, the rice husk in distiller's grains is usually centrifugally screened to remove most of the crude fibers, or microbial fermentation or enzymatic hydrolysis is used to degrade the crude fibers in distiller's grains into fiber distiller's grains. Sugar, glucose and other small molecular sugars that can be digested by monogastric animals, and at the same time degrade part of the crude protein in distiller's grains into small molecular peptides, which improves the nutritional value of distiller's grains to a certain extent;
[0003] The existing microbial fermentation and enzymatic hydrolysis methods still have some shortcomings: the degradation mechanism of lignin is different from that of cellulose and hemicellulose. Degradation of lignin, some can only effectively degrade cellulose and hemicellulose, only choose any one type of microorganisms to degrade crude fiber, can not get a higher degradation rate of crude fiber, for example, if only select the microorganisms that can degrade cellulose Fermentation with Trichoderma of hemicellulose, the lignin in the rice husk cannot be effectively degraded, and the lignin binds to cellulose and hemicellulose, thereby inhibiting the degradation of cellulose and hemicellulose, Make the overall degradation rate of crude fiber lower

Method used

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  • A method for preparing yeast culture based on distiller's grains and its application in animal feed
  • A method for preparing yeast culture based on distiller's grains and its application in animal feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for preparing yeast culture based on liquor distiller's grains, comprising the following steps:

[0037] (1) Preparation of solid-state fermentation raw materials: After fully mixing distiller's grains and wheat bran at a mass mixing ratio of 7:3, put them in an extruder, add 1% fermentation powder by weight of the mixture, and heat at 180-220°C Carry out puffing treatment, carry out secondary pulverization to the mixture after puffing, pass through 100 mesh sieves, add soybean meal and molasses of 10% by weight of puffed mixture respectively, mix evenly, set aside;

[0038](2) Preparation of seed solution: Weigh 2g of dry yeast into a 100ml Erlenmeyer flask, add distilled water at a temperature of 40°C and 1% glucose at a ratio of 1g:9ml, seal the seal, place it in a water bath at 40°C for 15 minutes, and activate it in a water bath at 30°C for 1 hour. It is the activated yeast seed solution;

[0039] (3) Inoculate the yeast seed solution prepared in step (2)...

Embodiment 2

[0042] Same as Example 1, the difference is that the mass mixing ratio of distiller's grains and wheat bran is 4:6.

Embodiment 3

[0044] Same as Example 2, the difference is that in step (1), a combined enzyme solution of 5% of the weight of the mixture is added to the puffed material, and after thorough mixing, the mixture is placed in an environment of 50°C and a relative humidity of 25-35% for 24 hours. ; Wherein the combined enzyme solution is configured by β-glucanase, xylitolase and cellulase according to the mass ratio of 1:1:2.

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Abstract

The invention relates to a method for preparing yeast culture based on liquor distiller's grains and its application in animal feed. The distiller's grains, a by-product of solid-state fermentation, are used as the main raw material, and wheat bran is added as an auxiliary material. After puffing, activated yeast is inoculated, and the solid-state fermentation process is used to produce yeast cultures to optimize the quality of fermentation culture products. The advantages of the present invention are: the solid-state fermentation process is simple to operate, can effectively reduce the use of water resources, thereby avoiding environmental pollution problems caused by waste water discharge; Feed additives are added to ordinary feed to improve the nutritional value of feed.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a preparation of yeast culture based on distiller's grains and its application in animal feed. Background technique [0002] Distiller's grains are the residues of sorghum, corn, wheat and other single raw materials or mixed raw materials after fermentation and distillation. They are feed raw materials with high nutritional value. Compared with raw materials, the content of crude protein and crude fat in distiller's grains has increased, the content of nitrogen-free extracts has decreased correspondingly, and the content of B vitamins is also higher. It can be added to the diet of livestock and poultry as roughage, but white distiller's grains Distiller's grains also contain a large amount of crude fiber, which mainly comes from the rice husk used as a filler in the liquor brewing process. These crude fibers include lignin, cellulose, and hemicellulose, which can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/38A23K10/37A23K10/14A23K20/22A23K10/33A23L5/20
CPCA23K10/12A23K10/14A23K10/33A23K10/37A23K10/38A23K20/22A23L5/28Y02P60/87
Inventor 彭楠刘玲常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV