Method for self-assembly of grass carp swimming-bladder collagen and collagen biological raw material
A swim bladder collagen and self-assembly technology, applied in the fields of peptide preparation, chemical instruments and methods, animal/human proteins, etc.
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Embodiment 1
[0028] A grass carp swim bladder collagen self-assembly method, comprising the following steps:
[0029] (1) Grass carp swim bladders were degreased and washed with water until neutral, added 0.5M acetic acid (1:10, w / v), 0.1% (w / v) 3000U / mg pepsin for 24 hours, centrifuged at 5000r / min for 20min, and Add pre-cooled sodium chloride to the supernatant for salting out, let it stand for 4 hours, and then centrifuge to collect the precipitate. used to obtain the grass carp swim bladder collagen.
[0030] (2) After the grass carp meat is minced, add 10 times the volume of pre-cooled sodium chloride solution (50mmol / L) to homogenate, place in an ice bath and stir for 30min, filter with gauze, centrifuge at 8000r / min for 15min, take the precipitate and The grass carp myofibrillar protein was obtained by adding pre-cooled sodium chloride solution for homogenization, centrifugation, and circular washing for 3 times.
[0031] (3) prepare 40mg / mL grass carp myofibrillar protein acetic ...
Embodiment 2
[0036] A grass carp swim bladder collagen self-assembly method, comprising the following steps:
[0037] (1) After the grass carp swim bladder is degreased, it is fully washed with water until it becomes neutral, and 0.5M acetic acid (1:10, w / v), 0.1% (w / v) 3000U / mg pepsin is added to react for 36h, centrifuged at 5000r / min for 20min, and Add pre-cooled sodium chloride to the supernatant for salting out, let it stand for 4 hours, and then centrifuge to collect the precipitate. use.
[0038] (2) Extraction of grass carp myofibrillar protein: After the grass carp meat is minced, add 10 times the volume of pre-cooled sodium chloride solution (60mmol / L) to homogenate, place it in an ice bath and stir for 30min, filter with gauze, 8000r / L Centrifuge at 1 min for 15 min, take the precipitate, add pre-cooled sodium chloride solution to homogenate, centrifuge, and cycle wash 3 times, the obtained precipitate is grass carp myofibrillar protein.
[0039] (3) Enzymatic hydrolysis of gr...
Embodiment 3
[0044] A grass carp swim bladder collagen self-assembly method, comprising the following steps:
[0045] (1) Grass carp swim bladders were degreased and washed with water until neutral, added 0.5M acetic acid (1:10, w / v) and 0.1% (w / v) 3000U / mg pepsin to react for 48h, centrifuged at 5000r / min for 20min, and Add pre-cooled sodium chloride to the supernatant for salting out, let it stand for 4 hours, and then centrifuge to collect the precipitate. use.
[0046] (2) Extraction of grass carp myofibrillar protein: After the grass carp meat is minced, add 10 times the volume of pre-cooled sodium chloride solution (70mmol / L) to homogenate, place it in an ice bath and stir for 30min, filter with gauze, 8000r / L Centrifuge at 1 min for 15 min, take the precipitate, add pre-cooled sodium chloride solution to homogenate, centrifuge, and cycle wash 3 times, the obtained precipitate is grass carp myofibrillar protein.
[0047] (3) Enzymatic hydrolysis of grass carp myofibrillar protein: ...
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