A self-assembled antimicrobial peptide
A technology of self-assembly and antimicrobial peptides, applied in the direction of antibacterial drugs, peptides, peptide/protein components, etc., can solve the problems of insufficient penetration, and achieve the effect of increasing water solubility, good self-assembly ability, excellent antibacterial activity and selectivity
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Embodiment 1
[0027] Synthesis of self-assembling antimicrobial peptides:
[0028] 1. Materials
[0029] (1) Take by weighing MBHA resin (toluene hydrogenamine resin) 0.7861g;
[0030] (2) Prepare a DMF (dimethylformamide) solution of the following substances at a concentration of 0.2mol / L:
[0031] Fmoc-Phe-OH (N-fluorenylmethoxycarbonyl-phenylalanine): volume 21mL, mass 1.63g;
[0032] Fmoc-Lys(Boc)-OH(N-fluorenylmethoxycarbonyl-N'-tert-butoxycarbonyl-lysine): volume 21mL, mass 1.97g;
[0033] Fmoc-Pro-OH (N-fluorenylmethoxycarbonyl-proline): volume 11mL, mass 0.74g;
[0034] Fmoc-Leu-OH (N-fluorenylmethoxycarbonyl-leucine): volume 21mL, mass 1.48g;
[0035] Fmoc-Gly-OH (N-fluorenylmethoxycarbonyl-glycine): volume 11mL, mass 0.65g;
[0036] Fmoc-Ala-OH (N-fluorenylmethoxycarbonyl-alanine): volume 11mL, mass 0.68g;
[0037] Fmoc-Arg(Pbf)-OH(N-Fremoxycarbonyl-2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl-arginine): volume 11mL, mass 1.43g;
[0038] Nap-CH 2 COOH: volume 11mL, m...
Embodiment 2
[0048] Self-assembled morphology and secondary structure of self-assembled antimicrobial peptides
[0049] (1) Prepare the phosphate buffer solution (pH 7.0, ionic strength 10mM) of the polypeptide molecule Nap-Phe-Phe-Lys-Pro-Leu-Gly-Leu-Ala-Arg-Lys, the concentrations of which are 0.25mM and 0.5mM respectively , 1.0mM and 2.0mM, placed at room temperature for 3 days, observed circular dichroism spectrum results and atomic force microscope results.
[0050] The results of the circular dichroism spectrum are as follows figure 1 As shown in a, it is found that the polypeptide molecule mainly presents a random coil secondary structure when the concentration is below 1.0 mM; at a concentration of 2.0 mM, it presents a β-sheet secondary structure.
[0051] Atomic force microscopy results such as figure 1 b. figure 1 As shown in c, it was found that under the concentration of 1.0mM polypeptide molecules, there were many random aggregates in the solution, but no regular self-asse...
Embodiment 3
[0056] Cell / bacterial culture test
[0057] (1) Cell experiment: first inoculate 100 μl in a sterile 96-well plate with a density of 1×10 5 Cells / mL of HeLa / NIH 3T3 cells, placed in a 37°C incubator for 24 hours, after they adhered to the wall, suck out the culture medium in the well plate, and add 100 μl of fresh culture medium and 100 μl of filtered different concentrations to each well The peptide solution, its absorbance is Abs peptide , 4 parallels were set up for each concentration, and the wells that only added Tris (trishydroxymethylaminomethane) buffer and no peptide were used as a control group, and the absorbance was Abs Tris , and then put the well plate back in the incubator at 37°C for 6h. After the effect was completed, 20 μl of MTT (3-(4,5-dimethylthiazole-2)-2 at a concentration of 5 mg / mL was added to each well. , 5-diphenyltetrazolium bromide) solution, continue culturing in the incubator for 4 hours, then suck out the liquid in the well plate, add 150 μl ...
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