Application of chemical small molecule 4-aminobiphenyl in prevention and treatment of osteoarthritis

An aminobiphenyl and osteoarthritis technology, applied in the field of cell medicine, can solve the problems of 4-aminobiphenyl arthritis, which have not been reported, and achieve the effect of improving cartilage regeneration ability and promoting cartilage repair and regeneration.

Pending Publication Date: 2019-11-05
深圳丹伦基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The 4-aminobiphenyl (4-ABP) involved in the present invention has no relevant reports in the research on the treatment of arthritis

Method used

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  • Application of chemical small molecule 4-aminobiphenyl in prevention and treatment of osteoarthritis
  • Application of chemical small molecule 4-aminobiphenyl in prevention and treatment of osteoarthritis
  • Application of chemical small molecule 4-aminobiphenyl in prevention and treatment of osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The medial meniscal instability model (DMM) in this example can simulate the cartilage defect caused by arthritis.

[0034] 1) Take 36 10-week-old male C57BL / 6J mice for anesthesia, prepare the skin and disinfect the skin, incise the skin along the inner side of the knee joint, peel off the muscle tissue, push the joint cavity ligament to the outside, expose the femur and tibia, and separate the joint cavity The adipose tissue is used to expose the medial meniscal ligament, which is then cut with fine scissors, the ligament and muscle tissue are restored, the muscle and skin are sutured, and the joint cavity is sealed.

[0035] 2) Intramuscular injection of penicillin sodium (22000U / 100g) was given after the operation to prevent infection.

[0036] 3) The 12 C57BL / 6J mice in the sham operation group were only sutured after the joint cavity was opened, and the ligaments were not cut.

Embodiment 2

[0038] Intra-articular injection for the treatment of cartilage defects.

[0039] 1) One month after the DMM operation, the DMM-modeled mice were randomly divided into 3 groups, 12 in each group, namely: Model group (DMM modeled), KGN group (100 μM KGN injected intra-articularly after DMM modeled). ), 4-ABP group (intra-articular injection of 100 μM 4-ABP after DMM modeling).

[0040] 2) Medication treatment: intra-articular injection once a week for 4 consecutive weeks.

Embodiment 3

[0042] Detection of regenerative repair of articular cartilage tissue.

[0043] (1) Safranin fast green staining of bone tissue sections.

[0044] 1) Bake slices at 50~60°C for 2 hours.

[0045] 2) Dewaxing and rehydration: dewax with xylene Ⅰ and Ⅱ for 10 minutes respectively, then rehydrate with 100% ethanol, 100% ethanol, 95% ethanol, 85% ethanol, 70% ethanol, and 50% ethanol for 5 minutes respectively, and rinse with running water 5min.

[0046] 3) Fast green staining: 0.05% fast green staining for 1~5min; 1% glacial acetic acid quick washing for 10s~1min; deionized water to clean the remaining acid solution, observe the fast green staining under a microscope, if the coloring is light, re-stain.

[0047] 4) Safranin staining: stain with 0.5% safranin O for 5-10 minutes; decolorize with 95% ethanol for 30 seconds, observe the staining condition under a microscope, if the staining is satisfactory, continue, otherwise wash with water and re-stain.

[0048] 5) Dehydration a...

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Abstract

The invention discloses an application of 4-aminobiphenyl (4-ABP) as a small molecule compound in treatment of osteoarthritis. The experiments show that topical application of 4-ABP can effectively repair cartilage damage caused by DMM in mice, increases the expression level of Collagen II, CD44, and CD105 protein, reduces the expression of Collagen X, and increases the thickness of cartilage andthe expression of a cartilage matrix. Therefore, the application proves that 4-ABP can effectively reduce cartilage damage during osteoarthritis and delay cartilage degeneration. The application of the 4-aminobiphenyl (4-ABP) small molecule compound provided by the invention in the treatment of osteoarthritis can provide a new solution for promoting damage cartilage regeneration for medical research and tissue engineering research. The application also includes the therapeutic effect of a tablet containing 4-ABP, an injection, a powder injection, and the like on cartilage degeneration and damage such as osteoarthritis.

Description

technical field [0001] The invention belongs to the field of cell medicine, in particular, the invention relates to the application of 4-aminobiphenyl (4-ABP) in reducing and repairing osteoarthritis damage. Background technique [0002] Osteoarthritis (OA) is mainly divided into two categories: traumatic OA caused by local trauma, or senile OA caused by individual aging, and the incidence of the latter is extremely high in the elderly and obese people. The main pathological features of OA are degeneration of articular cartilage, synovial inflammation, subchondral bone damage, bone remodeling, osteophyte formation, degradation of extracellular matrix, and other changes in joint structure, which eventually lead to joint pain, stiffness, and even motor function. Obstacles, etc., seriously affect the quality of life. [0003] Aging, genetic factors and mechanical stress are all risk factors for OA. The incidence of OA gradually increases with age; and the incidence of women i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/136A61P29/00A61P19/02A61P19/04
CPCA61K31/136A61P29/00A61P19/02A61P19/04
Inventor 张帅周海周光前李真莫翠萍
Owner 深圳丹伦基因科技有限公司
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