Trichina ES compound inducer induced DC vaccine for preventing, treating and diagnosing swine trichinosis
A technology for porcine trichinellosis and porcine trichinella, which is applied in the field of DC vaccines, can solve problems such as the lack of porcine DC vaccines, and achieve the effects of improving import and export quality, ensuring food safety, and avoiding negative effects
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experiment example 1
[0044] Experimental Example 1, Morphological Identification
[0045] 1. Phase-contrast inverted microscope identification: observe the growth state and morphological changes of the cells every day, and take photos of the cells on the 7th day.
[0046] 2. Scanning electric field identification: Centrifuge the collected suspended cells, remove 2 / 3 of the supernatant, and resuspend the cells; absorb the cell suspension, carefully drop it on the glass slide covered with a film, and place it flat in the culture dish to stand still 30min, then add 2.5% glutaraldehyde to the petri dish, immerse the slides in it, fix for 30min, rinse thoroughly with 0.1M buffer, fix with 1% osmic acid for 30min, rinse with buffer, start gradient dehydration with 30% alcohol, Transition with hexyl amyl acetate for 5 minutes, dry at the critical point, take out the glass slide and paste it on the specimen table with conductive glue, ion sputter coating; scan the electric environment to observe and take ...
experiment example 2
[0051] Experimental Example 2. Immunotherapy of Pig Trichinellosis
[0052] 1. Insect reduction rate of DC vaccine induced by ES complex inducer
[0053] According to 2000 Trichinella spiralis (International number ISS534 of Trichinella spiralis) / head respectively infects 40 healthy pigs, respectively induces DC vaccine treatment with normal saline, DC, ES and ES re-inducing agent to treat 10 pigs infected with Trichinella spiralis each; Pig per 100Kg body weight The dosage is 1ml;
[0054] Immunization strategy: After infection, all treated pigs were immunized with a single dose of DC vaccine on day 0 and day 2, and pigs were immunized with double doses on day 7, and immunized with double doses on days 13 and 17 treatment pigs;
[0055] After 18 days, the pigs in each group were killed, and the trichinella spiralis in the muscles and intestines of the pigs in each group were collected and counted by the digestion method, and the reduction rate was calculated according to t...
experiment example 3
[0063] Experimental example 3. hypericin and camptothecin concentration screening
[0064] Hypericin and camptothecin were used according to the six concentration groups of 8μg / mL, 1.6μg / mL, 0.32μg / mL, and 0.064μg / mL to act on wind and pig fetal fibroblasts (G418), and each concentration was set for 24h , 48h, and 72h three time groups, and set solvent control wells and blank wells, and set 3 parallel wells for each group. The concentration of the drug that is 5% toxic to the cells (that is, the concentration of the drug when the cell survival rate is 95%) is taken as the basic non-toxic concentration of the drug to the cells, and the concentration at 24 hours is used for subsequent experiments. Cell survival rate=(OD value experimental group-OD value control group) / (OD value control group-OD value blank group)×100%. see results Figure 4 and Figure 5 .
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