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Klebsiella sp. P5 with phosphorus dissolving function and application of Klebsiella sp. P5

A Klebsiella bacterium and phosphorus-dissolving technology, applied in the direction of application, chemicals for biological control, bacteria, etc., can solve the problems of aggravating environmental pollution and waste of phosphorus resources, so as to increase crop yield, reduce usage, The effect of improving the utilization rate

Active Publication Date: 2019-11-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Increased application of phosphorus fertilizers not only causes a large waste of phosphorus resources, but also aggravates the problem of environmental pollution

Method used

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  • Klebsiella sp. P5 with phosphorus dissolving function and application of Klebsiella sp. P5
  • Klebsiella sp. P5 with phosphorus dissolving function and application of Klebsiella sp. P5
  • Klebsiella sp. P5 with phosphorus dissolving function and application of Klebsiella sp. P5

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The screening of embodiment 1 phosphate-dissolving bacteria

[0042]1. Primary screening of phosphate-solubilizing bacteria

[0043] In September 2017, wheat plants that had grown for about 7 months were collected from the farmland in Yangling District, Shaanxi Province, and the loosely bound soil at the roots of the wheat was shaken vigorously. Cut off the root of the plant and put it into a 10mL sterilized centrifuge tube, inject 5mL of sterile water and perform ultrasonic treatment to dissolve the rhizosphere soil closely combined with the root of the plant in the water. The collected soil samples are yellow loam, with 24.03mg / kg of available phosphorus, 126.17mg / kg of available potassium, 56.28mg / kg of alkaline nitrogen, 21.06g / kg of organic matter, and pH=8.27.

[0044] Make a series of 10-fold dilutions of the aqueous solution, and take 5 μL of the dilution to be 10 times -5 、10 -6 and 10 -7 The soil suspension was spread on PKO inorganic phosphorus medium and...

Embodiment 2

[0057] Identification of embodiment 2 bacterial strain P5

[0058] 1. Morphological characteristics

[0059] The P5 strain was prepared into a bacterial suspension, diluted and spread on LB solid medium, and cultured at 28°C for 24 hours to observe the colony and cell morphology.

[0060] The results showed that the Gram staining of the bacteria was negative, the cells were short rod-shaped, and did not produce spores; after 24 hours of culture on LB medium, the colonies were round, with neat edges, 2-3mm in diameter, milky white and slightly pale in the center. yellow.

[0061] 2. Physiological and biochemical characteristics

[0062] Refer to the bacterial identification manual to measure the physiological and biochemical characteristics of the strain P5, such as VP reaction, carbon source utilization, and indole test. Indole test and methyl red test were negative, VP reaction was positive, can produce amylase and urease.

[0063] 3. 16S rDNA sequencing

[0064] Genomic...

Embodiment 3

[0067] Embodiment 3 wheat growth promoting test

[0068] Select wheat seeds with plump grains, sterilize the surface with 70% ethanol, rinse with sterile water at least 6 times, and apply the sterile water of the last rinse on the beef extract peptone solid medium to check whether the surface of the wheat is thoroughly sterilized. Sow the sterilized wheat seeds in the soil of the flower pot, plant 6 seeds in each pot, and cover the surface with about 1 cm of soil. Two treatments were set up in the experiment. In treatment 1, each plant was inoculated with 1 mL of sterile medium, and in treatment 2, each plant was inoculated with P5 bacterial solution (about 10 8 per mL) 1 mL, and each treatment was replicated 3 times. The flower pots are placed in the greenhouse, and the cultivation conditions are set as light 16h / 8h, temperature 25°C / 18°C. After the wheat seedlings emerged, water was poured every 5 days, and the plant height, fresh weight, dry weight, and nitrogen, phosphor...

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Abstract

The invention belongs to the technical field of plant rhizosphere growth-promoting bacteria and particularly discloses Klebsiella sp. P5 with a phosphorus dissolving function and application of the Klebsiella sp. P5. The Klebsiella sp. CCNWSX1902 P5 is collected in the China Center for Type Culture Collection (CCTCC) on April 22, 2019, with the collection number of CCTCC NO:M 2019280. The phosphorus dissolving bacterium P5 is separated from wheat rhizosphere soil and identified as the Klebsiella sp. CCNWSX1902 in the aspects of morphology, physiological-biochemical characteristics and genetics. The strain can effectively dissolve indissolvable phosphorus in plant rhizosphere soil, substantially increase the content of rapidly available phosphorus in the soil and increase the utilization rate of phosphorus in the soil by plants to promote plant growth, reduce the dosage of chemical fertilizer and increase the yield of crops, and has important significance in agricultural production.

Description

technical field [0001] The invention relates to the technical field of plant rhizosphere growth-promoting bacteria, in particular to a Klebsiella P5 strain capable of dissolving phosphorus and its application. Background technique [0002] Phosphorus is an essential nutrient element for plant growth and metabolic activities, and plays a key role in the high yield of crops. Relevant literature shows that 74% of the cultivated land in my country is deficient in phosphorus, and 95% of the phosphorus in the soil combines with calcium, aluminum, iron and other ions to form insoluble compounds, which are difficult for plants to absorb and utilize. In agricultural production, the use of highly water-soluble phosphate fertilizers is often used to supplement the needs of plants for phosphorus. However, after phosphate fertilizers are applied to the soil, insoluble phosphates are easily formed and quickly absorbed by soil minerals or fixed by microorganisms. The utilization rate is lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/02A01P21/00C09K101/00C12R1/22
CPCC09K17/14C09K2101/00C12R2001/22C12N1/205Y02A40/20
Inventor 李哲斐王娟娟
Owner NORTHWEST A & F UNIV
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