Step-by-step reaction type electrochemical detection method and device

A detection method and detection device technology, applied in the field of electrochemical detection, can solve problems such as incomplete substrate reaction, influence on electrical signal detection, and failure to contact the electrode surface, etc., to achieve high detection sensitivity, improve detection accuracy, and enzymatic reaction full effect

Pending Publication Date: 2019-11-15
SHANDONG IND TECH RES INST OF ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If there are too many kinds of interfering substances, the modification of various enzymes on the surface of the electrode will have a great impact on the enzyme activity and the enzyme reaction. It may affect the enzyme activity, and may also cause the enzyme to not touch the electrode surface after reacting with the substrate, which will affect the detection of electrical signals; and the channel area of ​​the electrode suction port is limited, and various enzyme solutions are fixed on the electrode surface. It will cause incomplete substrate reaction, which greatly increases the cost of research and development for enterprises, and also easily leads to high cost of electrode test pieces.

Method used

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  • Step-by-step reaction type electrochemical detection method and device
  • Step-by-step reaction type electrochemical detection method and device
  • Step-by-step reaction type electrochemical detection method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0057] When testing the uric acid content in the blood, uric acid is oxidized by urate oxidase on the electrode to generate H 2 o 2 ,

[0058] 2 molecules of hydrogen peroxide + mediator (reduction) mediator (oxidation) + 4 molecules of water

[0059] Electronic sensor + medium → formation of electric current

[0060] However, ascorbic acid in the blood has a strong reducing ability, competitively reducing the produced H 2 o 2 , so that the generation of colored substance quinone imine is reduced, which will cause the final detection result to be low. Therefore, ascorbate oxidase can be stored in the sampling tube. At this time, ascorbate oxidase is the reactant corresponding to the interference. First, the blood to be tested is sucked into the sampling tube to fully react with ascorbate oxidase. Ascorbic acid is oxidized and destroyed to eliminate interference, and the reacted sample is dropped onto the electrode modified with uricase to detect the reaction signal, and u...

Embodiment 2

[0062] When testing blood cholesterol levels the reaction is:

[0063] Cholesterol (CE)+H 2 O Cholesterol + Fatty Acid

[0064] Cholesterol+O 2 +H 2 O Cholesterone + Hydrogen Peroxide

[0065] 2 molecules of hydrogen peroxide + mediator (reduction) mediator (oxidation) + 4 molecules of water

[0066] Electronic sensor + mediator → formation of electric current, so two cholesterolases need to be modified for enzymatic reaction.

[0067] Therefore, cholesterol esterase can be stored in the sampling tube. At this time, cholesterol esterase is the corresponding reactant of the intermediate product, cholesterol oxidase is modified on the electrode, cholesterol oxidase is the corresponding reactant of the sample to be tested, and blood to be tested is sucked into the sampling tube. The cholesterol ester in the blood is catalyzed by the cholesterol esterase in the sampling tube to generate cholesterol, and then the sample is dropped onto the electrode modified with cholesterol o...

Embodiment 3

[0074] When the uric acid content in the blood needs to remove the interference of ascorbic acid, use a dropper to store ascorbate oxidase. At this time, ascorbate oxidase is the reactant corresponding to the interference. Place the sample to be tested in the sample cup, and pour the enzyme solution in the dropper into the In the sample cup, after repeated suction and discharge for mixed reaction, the sample after suction and reaction is dropped onto the electrode modified with uricase to detect electrical signals. After verification, it was found that the detection results were consistent with the experimental results in Example 1, indicating that the form of the sampling tube had no influence on the detection results.

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Abstract

The invention discloses a step-by-step reaction type electrochemical detection method, and the method comprises the following steps: S1: modifying a blank reactant or at least one functional reactantby an electrode, and storing the at least one functional reactant by a sampling tube, wherein the sum of the number of the functional reactants modified by the electrode and the number of the functional reactants stored by the sampling tube is the number of the functional reactants for completing the parameter determination of a sample to be detected; and S2: the sample to be detected reacting with the functional reactants stored in the sampling tube, and the reacted sample reacting with the blank reactant or the functional reactants modified by the electrode to generate a detectable electrochemical active product. The invention also discloses a step-by-step reaction type electrochemical detection device. The step-by-step reaction type electrochemical detection method can fully remove interference, has more full reaction before detection and high detection sensitivity, improves the accuracy of the electrode and increases the anti-interference diversity of the electrode; and various enzyme liquid modification conditions are not required to be considered, so that the manufacturing cost is reduced, and the manufacturing conditions are simplified.

Description

technical field [0001] The invention relates to the field of electrochemical detection, more specifically, it relates to a step-by-step reaction type electrochemical detection method and device. Background technique [0002] The following background art is provided to help the reader understand the present invention and is not to be admitted as prior art. [0003] The main purpose of biosensor research is to develop biosensors with practical application capabilities and their corresponding preparation techniques. The application of the screen printing technology makes it possible to prepare disposable electrochemical biosensor electrodes in batches. The method is simple in process and low in cost, and is suitable for industrial production of electrodes. Therefore, screen-printed electrodes based on the principle of electrochemical biosensors are widely used in the field of point-of-care detection (POCT). Physiological fluids such as blood, urine, sweat, and saliva contain ...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327G01N1/14
CPCG01N27/26G01N27/3271G01N1/14G01N2001/1427G01N2001/1418
Inventor 梁波叶学松赵欢萍
Owner SHANDONG IND TECH RES INST OF ZHEJIANG UNIV
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