Step-by-step reaction type electrochemical detection method and device

Abstract

The invention discloses a step-by-step reaction type electrochemical detection method, and the method comprises the following steps: S1: modifying a blank reactant or at least one functional reactantby an electrode, and storing the at least one functional reactant by a sampling tube, wherein the sum of the number of the functional reactants modified by the electrode and the number of the functional reactants stored by the sampling tube is the number of the functional reactants for completing the parameter determination of a sample to be detected; and S2: the sample to be detected reacting with the functional reactants stored in the sampling tube, and the reacted sample reacting with the blank reactant or the functional reactants modified by the electrode to generate a detectable electrochemical active product. The invention also discloses a step-by-step reaction type electrochemical detection device. The step-by-step reaction type electrochemical detection method can fully remove interference, has more full reaction before detection and high detection sensitivity, improves the accuracy of the electrode and increases the anti-interference diversity of the electrode; and various enzyme liquid modification conditions are not required to be considered, so that the manufacturing cost is reduced, and the manufacturing conditions are simplified.

Description

technical field [0001] The invention relates to the field of electrochemical detection, more specifically, it relates to a step-by-step reaction type electrochemical detection method and device. Background technique [0002] The following background art is provided to help the reader understand the present invention and is not to be admitted as prior art. [0003] The main purpose of biosensor research is to develop biosensors with practical application capabilities and their corresponding preparation techniques. The application of the screen printing technology makes it possible to prepare disposable electrochemical biosensor electrodes in batches. The method is simple in process and low in cost, and is suitable for industrial production of electrodes. Therefore, screen-printed electrodes based on the principle of electrochemical biosensors are widely used in the field of point-of-care detection (POCT). Physiological fluids such as blood, urine, sweat, and saliva contain ...

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Problems solved by technology

If there are too many kinds of interfering substances, the modification of various enzymes on the surface of the electrode will have a great impact on the enzyme activity and the enzyme reaction. It may affect the enzyme activity, and may also cause the enzyme to not touch the electrode surface after reacting with the substrate, which will affect the detection of electrical signals; and the channel area of ​​the electrode suction port is limited, and various enzyme solutions are fixed on the electrode surface. It will cause incomplete substrate reaction, which greatly increases the cost of research and development for enterprises, and also easily leads to high cost of electrode test pieces.

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