Method for determining concentration of robustaside B in blood plasma

A technology of oujinin and blood plasma, which is applied in the field of determination of oujinin concentration in plasma, and achieves the effects of strong specificity, high sensitivity, and simple pretreatment method

Inactive Publication Date: 2019-11-15
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the in vivo determination method of oujinin

Method used

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  • Method for determining concentration of robustaside B in blood plasma
  • Method for determining concentration of robustaside B in blood plasma
  • Method for determining concentration of robustaside B in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Determination of Urinin Concentration in Rat Plasma

[0030] 1. Experimental materials and instruments

[0031] Both oujinin (MUST-18081702) and naringenin (MUST-19032406) were purchased from Chengdu Mansite Biotechnology Co., Ltd. Test water: ultrapure water; methanol: chromatographically pure (Merck Company).

[0032] API 4000LC-MS / MS triple quadrupole mass spectrometer, equipped with electrospray ionization source (ESI), chromatography workstation: Analyst 1.6; Agilent 1200 high performance liquid chromatography; AE240 electronic balance (Shanghai Mettler-Toledo Co., Ltd. company); MICRO-17R refrigerated centrifuge (Thermo, USA); WH-2 micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory); Drict-Q5 ultrapure water machine (Millipore, France).

[0033] 2. Liquid conditions

[0034] 1. Liquid chromatography conditions

[0035] Chromatographic column: Waters XTerra MS C18 column (dp 3.5μm, 50mm ID×2.1mm); mobile phase: acetonitrile-2mmol L -1 Ammonium acet...

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Abstract

The invention discloses a method for determining concentration of robustaside B in blood plasma. A liquid chromatography-mass spectrometry system is adopted for determination, firstly, a to-be-determined sample is taken, a certain quantity of organic solvent protein precipitation solutions are added, a chromatographic column is used for separation after pretreatment, and a mass spectrometry detector is used for detection. The method is rapid, accurate, high in sensitivity, simple and convenient to operate and suitable for determining the concentration of robustaside B in the blood plasma.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for measuring the concentration of oujinin in blood plasma. Background technique [0002] Robustaside B is a new phenylpropanoid compound isolated from the whole herb of Pedicularis dichotoma in the genus Pedicularis dichotoma. In vitro experiments have proved that oujinin has significant anti-oxidation, anti-fatigue and other pharmacological activities, and is a new drug source compound with good development prospects. Its structural formula is as follows: [0003] [0004] At present, there is no literature report on the in vivo determination method of oujinin. The invention establishes a method for measuring oujinin in rats, and conducts exploratory research on the drug metabolism of oujinin through the method. Contents of the invention [0005] Purpose of the invention: Aiming at the problems existing in the prior art, the present inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 戴国梁张农山居文政刘史佳高骁君于茜吴磊
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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