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Thermo-sensitive type chitosan hydrogel entrapped cell material as well as preparation method and application

A temperature-sensitive chitosan technology, applied in the field of medicine, can solve the problems of limited application, cytotoxic tissue damage, high permeability, etc., and achieve the goals of avoiding cytotoxicity, reducing tissue damage, and good thermal stability Effect

Inactive Publication Date: 2019-11-19
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to ensure the successful gelation of chitosan / sodium β-glycerophosphate and the mechanical strength of the gel, a high concentration of β-sodium glycerophosphate is usually required, and a 56% aqueous solution of β-sodium glycerophosphate is generally used, and the final hydrogel The content of β-sodium glycerophosphate in the system is also greater than 6%, which will lead to high permeability, cytotoxicity and tissue damage, which largely limits its further application in the fields of biomedicine and tissue engineering

Method used

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Examples

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Effect test

preparation example Construction

[0027] The present invention also provides a method for preparing the above-mentioned temperature-sensitive chitosan hydrogel-encapsulated cell material, comprising the following steps:

[0028] 1) Add the low-viscosity chitosan powder into deionized water, ultrasonically disperse until uniform, then add lactic acid while stirring until the low-viscosity chitosan is completely dissolved, and obtain an aqueous solution of chitosan lactic acid;

[0029] 2) Mixing the chitosan lactic acid aqueous solution prepared in step 1) and sodium β-glycerophosphate solution at 0-4° C. to obtain a temperature-sensitive chitosan hydrogel-encapsulated cell material.

[0030] Specifically, the present invention dissolves low-viscosity chitosan powder in deionized water, ultrasonically disperses until uniform, and then adds lactic acid while stirring until the low-viscosity chitosan is completely dissolved. The purpose is to completely dissolve the low-viscosity shell with a minimum amount of lac...

Embodiment 1

[0036]Accurately weigh 3g of chitosan powder, 196g of deionized water and 1.3g of lactic acid, stir to dissolve fully, let stand to remove foam, filter to remove insoluble matter, and store in a 4°C refrigerator for later use. Accurately weigh 2.7g of sodium β-glycerophosphate and dissolve in 25mL of deionized water to prepare a 10.8% aqueous solution of sodium β-glycerophosphate, filter and store at 4°C for use. Take 12g of chitosan solution and 4g of β-sodium glycerophosphate and mix them evenly under the low temperature condition of 0-4°C. Then mix the cell solution with the above mixture, inject into the body or culture the cells in 3D in vitro at 37°C. The experimental results show that the chitosan hydrogel-encapsulated cell material prepared in Example 1 can be gelled at 37°C.

Embodiment 2

[0038] Accurately weigh 2.5g of chitosan powder, 196.5g of deionized water and 1.19g of lactic acid, stir to dissolve fully, let stand to defoam, filter to remove insoluble matter, and store in a 4°C refrigerator for later use. Accurately weigh 2.7g of sodium β-glycerophosphate and dissolve in 25mL of deionized water to prepare a 10.8% aqueous solution of sodium β-glycerophosphate, filter and store at 4°C for use. Take 12g of chitosan solution and 3.5g of beta-glycerophosphate sodium and mix them evenly under the low temperature condition of 0-4°C. Then mix the cell solution with the above mixture, inject into the body or culture the cells in 3D in vitro at 37°C. The experimental results show that the chitosan hydrogel-encapsulated cell material prepared in Example 2 can be gelled at 37°C.

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Abstract

The invention discloses a thermo-sensitive type chitosan hydrogel entrapped cell material as well as a preparation method and application and belongs to the technical field of medicine biologics. Thehydrogel entrapped cell material disclosed by the invention is prepared from the following raw materials in percentage by mass at 0-4 DEG C: 0.8-1.5% of low-viscosity chitosan, 0.4-0.5% of lactic acid, 1-3% of beta-sodium glycerophosphate and the balance of water. The hydrogel entrapped cell material disclosed by the invention is capable of entrapping cells to form gel at 37 DEG C. Compared with conventional chitosan / beta-sodium glycerophosphate thermo-sensitive hydrogel, the amount of the beta-sodium glycerophosphate can be effectively reduced, the toxicity of the high permeability of the hydrogel upon cells can be avoided, and in-vivo or in-vitro 3D cell culture in an environment of 37 DEG C after cell entrapping and injection can be achieved. The material has the advantages of being simple in reaction condition, short in response time, good in gel thermal stability, free of cytotoxicity, good in biocompatibility, and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a temperature-sensitive chitosan hydrogel-encapsulated cell material, a preparation method and application thereof. [0002] technical background [0003] Glycerophosphate (GP), an organic compound that occurs naturally in the body, including alpha-glycerophosphate (α-GP) and beta-glycerophosphate (β-GP), is commonly used as phosphoric acid to treat imbalances in phosphate metabolism Salt Source, whose drug administration is FDA-approved. Sodium glycerophosphate has also been shown to be useful as a catalyst to induce a sol-gel transition in chitosan solutions at physiological pH and temperature. [0004] Chitosan (CS), also known as deacetylated chitin, is obtained through deacetylation of chitin, which exists widely in nature. Chitosan has the advantages of biocompatibility, biodegradability, non-toxicity, etc., and studies have shown that chitosan has very high m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/12A61K47/24A61K47/36A61K35/12A61L27/38A61L27/20A61L27/00A61L27/50A61L27/52C12N11/10C12N11/04
CPCA61K9/0019A61K9/06A61K35/12A61K47/12A61K47/24A61K47/36A61L27/00A61L27/20A61L27/38A61L27/50A61L27/52A61L2400/06C12N11/04C12N11/10C08L5/08
Inventor 杨宇民王勇军凌珏汤晓璇张鲁中
Owner NANTONG UNIVERSITY
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