A simulated moving bed continuous chromatography system and its application and method for purifying coenzyme q10
A technology that simulates moving bed and chromatographic chromatography, which is applied in the field of coenzyme Q10 purification, can solve the problems of low yield of coenzyme Q10, shortened packing life, impurity cannot be desorbed cleanly, etc., so as to reduce production cost, improve utilization rate and reduce labor intensity Effect
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Embodiment 1
[0047] Dissolve the multi-component coenzyme Q10 crude extract (among them, the impurities mainly contain coenzyme Q9, reduced coenzyme Q9, 5-demethoxy coenzyme Q10, reduced coenzyme Q10, coenzyme Q11, reduced coenzyme Q11) in n-hexane In the preparation method, a feed solution with a coenzyme Q10 solid concentration of 80 mg / mL was prepared, and the content of coenzyme Q10 was about 75.5%. like figure 1 As shown, the simulated moving bed continuous chromatography system is equipped with 6 chromatographic columns, including 2 in the feed area, 1 in the elution area, 2 in the desorption area and 1 in the regeneration area, all of which have a size of 3.5cm×50cm; The stationary phases are all silica gel (particle size 45 μm, pore size 10 nm); the eluent is a mixture of cyclohexane and ethyl acetate, wherein the volume percentage of ethyl acetate in 1# eluent is 8%, and 2# elutes The volume percentage of ethyl acetate in the eluent is 35%, and the volume percentage of ethyl acet...
Embodiment 2
[0049] The two-component coenzyme Q10 crude extract (wherein the impurity is coenzyme Q11) was dissolved in n-hexane to prepare a feed solution with a coenzyme Q10 solid concentration of 120 mg / mL, wherein the content of coenzyme Q10 was about 70.3%. The simulated moving bed is equipped with 12 chromatographic columns, including 4 in the feed area, 2 in the elution area, 4 in the desorption area and 2 in the regeneration area, all of which have a size of 3.5 cm × 50 cm; the stationary phases are all silica gel (particle size 25μm, pore size 20nm); the eluent is a mixture of petroleum ether and ethyl acetate, wherein the volume percentage of ethyl acetate in 1#, 2#, and 3# eluents is 10%; the operating temperature is 30°C; The parameters were optimized and determined as: eluent flow rate 6L / h, feed liquid flow rate 4L / h, switching time 0.5h. A high-concentration eluate rich in coenzyme Q10 is collected from the outlet of the elution zone. The analysis showed that the purity of...
Embodiment 3
[0051] Dissolve the multi-component coenzyme Q10 crude extract (among them, the impurities mainly contain coenzyme Q9, reduced coenzyme Q9, 5-demethoxy coenzyme Q10, reduced coenzyme Q10, coenzyme Q11, reduced coenzyme Q11) in n-hexane In the preparation method, a feed solution with a coenzyme Q10 solid concentration of 200 mg / mL was prepared, wherein the content of coenzyme Q10 was about 65.3%. The simulated moving bed is equipped with 18 chromatographic columns, including 5 in the feed zone, 5 in the elution zone, 4 in the desorption zone and 4 in the regeneration zone, all of which have a size of 3.5cm×50cm; the stationary phases are all alumina (grain Diameter 20 μm, pore diameter 22nm); The eluent is the mixture of n-hexane and acetone, wherein, the volume percent of acetone in the 1# eluent is 3%, the volume percent of acetone in the 2# eluent is 20%, 3# The eluent is pure n-hexane; the operating temperature is 32°C; the operating parameters are optimized and determined ...
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