A microfluidic chip for cell sorting based on coaxial dual-waveguide optical fiber

Abstract

The invention provides a cell sorting microfluidic chip based on a coaxial double waveguide optical fiber. Its features are: it consists of a base 1, lower limit holes (2, 7, 9, 13), tissue fluid injection tubes (3, 5), cell injection tube 4, connection hole 6, coaxial dual waveguide optical fiber fixing tube 8, cover Plate fixing groove 10, waste liquid discharge pipe 11, cell discharge pipe 12, interstitial fluid channel (15, 17), cell channel 16, coaxial dual waveguide fiber optic channel 18, waste liquid channel 19, waste liquid pool 20, cell sorting channel (21,24,26), cell storage pool (22,23,25), arc notch 28, upper limit hole (29,31,32,33), bayonet plate 30 and cover plate 27 are formed. The invention can be used for simultaneous sorting of multiple cells, and can be widely used in the fields of cell sorting, analysis and the like.

Description

(1) Technical field [0001] The invention relates to a cell sorting microfluidic chip based on a coaxial double waveguide optical fiber, which can be used for cell sorting and belongs to the technical field of microfluidic chips. (2) Background technology [0002] In 1975, a miniaturized gas chromatography device came out (Terry S C, Jerman J H, Angell J B.Agas chromatographic air analyzer fabricated on a silicon wafer[J].IEEE Transactions on Electron Devices,1979,26(12):1880- 1886.), laid the foundation for the miniaturization of instruments. In the 1990s, Manz et al first proposed the concept of miniature total analysis system (Manz A, Graber N, Widmer H M. Miniaturized Total Chemical-Analysis Systems—A Novel Concept for Chemical Sensing[J]. Sensors and Actuators B Chemical, 1990 , 1(1-6):244-248.). [0003] The micro total analysis system is also called "lab on a chip", also known as "microfluidic chip", and its main features are integration and miniaturization. It inte...

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Problems solved by technology

[0008] Although the microfluidic chip designed based on the principle of electroosmotic flow has the advantages of simple structure and easy portability, high-voltage electric fields are relatively easy to damage the internal structure of cells, and even lead to the inactivation of cell membranes in severe cases, resulting in false negative results.

In addition, the generation of electroosmotic flow requires the fabrication of electrodes, which increases the complexity of the chip

The microfluidic chip, which realizes cell sorting based on the difference in physical size, gets rid of the dependence of conventional methods on the subjective experience of pathologists, and is non-invasive in the whole process, but its structure is complex, especially the processing of columnar protrusions arranged in an arc is extremely difficult

The application of dielectrophoresis to sort cells is to move a cell that is not charged itself but can be polarized to different degrees in an uneven electric field. This method is accurate and will not destroy the object to be detected, and is easy It has the advantages of integrating with other devices, but this method is greatly affected by the external electric field and is prone to errors

Moreover, most microfluidic chips designed based on dielectrophoresis method also need to integrate electrodes in the microfluidic chip, which greatly increases the difficulty of processing.

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