Application of heparin sodium in preparation of diluent for goat sperm cryopreservation and preparation method

Abstract

The invention discloses an application of heparin sodium in preparation of a diluent for goat sperm cryopreservation and a preparation method, and also provides a diluent for goat sperm cryopreservation, which comprises heparin sodium, nutrient substances, buffer substances, a cryoprotectant and antibacterial substances. The diluent for goat sperm cryopreservation plays a role in protecting spermsin the processes of sperm cooling, cryopreservation and unfreezing, damage is reduced, and the sperm motility and acrosome integrity rate of goat frozen sperms are obviously improved.

Description

technical field

[0001] The invention belongs to the field of animal biotechnology and relates to animal reproduction technology, in particular to the application and preparation method of heparin sodium for preparing diluent for goat semen preservation. Background technique

[0002] Semen cryopreservation is one of the key links in the animal biotechnology system, which mainly solves the problem of long-term stable storage of semen. Through the artificial collection and long-term storage of semen, the semen produced by animals can be used across time, across regions, and in various ways, thus greatly improving the use value of male animals in the field of actual production and scientific research. For example, in the field of animal breeding, it is of great significance for estimation of breeding value, protection of germplasm resources, variety improvement, breeding of new varieties, realization of factory-scale farming, etc., as well as reducing production costs and improv...