Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence

A chemiluminescence and fluorescent carbon dot technology, applied to the application field of fluorescent materials in chemiluminescence, can solve the problems of complex preparation, high cost, poor compatibility, etc., and achieve the effects of simple detection equipment, low biological toxicity, and high application potential.

Inactive Publication Date: 2019-11-22
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide an application of a fluorescent material based on fluorescent carbon dots in chemiluminescence, s

Method used

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  • Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence
  • Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence
  • Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence

Examples

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Example Embodiment

[0029] Example 1. Preparation of fluorescent carbon dots by hydrothermal method

[0030] Step A. Weigh 1g of citric acid with a mass fraction of 99% and 2g of urea, add 10mL of azodicarbonamide (DMF), and perform ultrasonic vibration at room temperature to obtain a mixed solution;

[0031] Step B. Place the oscillated mixed solution in a high-temperature reaction furnace, heat it to 160°C, keep it warm for 8 hours and then naturally cool to room temperature;

[0032] Step C. Dissolve the reacted mixed solution in DMF, use a silica gel column (200 mesh) to obtain a red fluorescent carbon dots (r-CDs) solution, add excess absolute ethanol, filter, and retain the supernatant. Put it in a 60℃ vacuum drying oven for drying, which is the required red fluorescent carbon dots.

[0033] Replace the DMF in step A with azodimethylacetamide (DMAC), the other steps remain unchanged, and the desired green fluorescent carbon dots (g-CDs) can be obtained.

[0034] Replace the DMF in step A with deioni...

Example Embodiment

[0036] Example 2. Preparation of fluorescent carbon dots by hydrothermal method

[0037] Step A. Weigh 1g of citric acid with a mass fraction of 99% and 4g of urea, add 10mL of azodicarbonamide (DMF), and perform ultrasonic vibration at room temperature to obtain a mixed solution;

[0038] Step B. Place the oscillated mixed solution in a high-temperature reaction furnace, heat it to 200°C, keep it warm for 4 hours and then naturally cool to room temperature;

[0039] Step C. Dissolve the reacted mixed solution in DMF, use a silica gel column (200 mesh) to obtain a red fluorescent carbon dots (r-CDs) solution, add excess absolute ethanol, filter, and retain the supernatant. Put it in a 60℃ vacuum drying oven for drying, which is the required red fluorescent carbon dots.

[0040] Replace the DMF in step A with azodimethylacetamide (DMAC), the other steps remain unchanged, and the desired green fluorescent carbon dots (g-CDs) can be obtained.

[0041] Replace the DMF in step A with deioni...

Example Embodiment

[0042] Example 3. Preparation of fluorescent carbon dots by microwave method

[0043] Step A. Weigh 0.8g of citric acid and 1.6g of urea and dissolve in deionized water, and perform ultrasonic vibration at room temperature for uniform dispersion to obtain a mixed solution;

[0044] Step B. Place the mixed solution in a microwave reactor, and after microwave heating for 5 minutes, the solution turns from colorless to brown solid;

[0045] Step C. Dissolve the reacted solids in an excess of absolute ethanol, oscillate uniformly by ultrasonic, then repeat centrifugation three times, retain the supernatant, and dry it in a vacuum drying oven at 60°C to obtain the desired fluorescent carbon dots.

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Abstract

The invention belongs to the technical field of application of fluorescent carbon nano materials, and particularly relates to application of a fluorescent material based on fluorescent carbon dots inchemiluminescence. The fluorescent material is a combination of fluorescent carbon dots and a bis(oxalate)-hydrogen peroxide system, replaces traditional fluorescent dye macromolecules to realize high-brightness and long-lifetime chemiluminescence of the bis(oxalate)-hydrogen peroxide system, avoids contact carcinogenicity of the fluorescent dye macromolecules, and has good biocompatibility and environmental safety. The fluorescent carbon dots can be prepared by a hydrothermal method or a microwave method, and the fluorescent material can be applied to chemiluminescence cold light sources, chemiluminescence analysis or chemiluminescence imaging, and has the advantages of low biotoxicity, high detection sensitivity and high imaging sensitivity.

Description

technical field [0001] The invention belongs to the technical field of application of fluorescent carbon nanomaterials, and in particular relates to the application of a fluorescent material based on fluorescent carbon dots in chemiluminescence. Background technique [0002] The double oxalate chemiluminescence system is known to be the most efficient chemiluminescence system except bioluminescence in nature, and its luminescence application has made important research progress in chemiluminescence cold light source, chemical analysis and detection, chemiluminescence bioimaging, etc. Generally speaking, the bisoxalate-hydrogen peroxide chemiluminescent system is an indirect luminescent system, and its luminescence requires a luminescent medium. Currently, the commonly used chemiluminescent molecules in this chemiluminescent system are fluorescent small molecules such as perylene, rhodamine B, rhodamine G, and rubrene. However, such chemiluminescent dyes generally have certa...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00G01N21/6428
Inventor 娄庆沈成龙单崇新
Owner ZHENGZHOU UNIV
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