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Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence

A chemiluminescence and fluorescent carbon dot technology, applied to the application field of fluorescent materials in chemiluminescence, can solve the problems of complex preparation, high cost, poor compatibility, etc., and achieve the effects of simple detection equipment, low biological toxicity, and high application potential.

Inactive Publication Date: 2019-11-22
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide an application of a fluorescent material based on fluorescent carbon dots in chemiluminescence, so as to solve the problems in the prior art that chemiluminescent bodies have biological toxicity, complicated preparation, high cost and poor compatibility

Method used

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  • Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence
  • Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence
  • Application of fluorescent material based on fluorescent carbon dots in chemiluminiscence

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1. Preparation of fluorescent carbon dots by hydrothermal method

[0030] Step A. Weigh 1 g of citric acid with a mass fraction of 99% and 2 g of urea, add it to 10 mL of nitrogen-nitrogen dimethylformamide (DMF), and perform ultrasonic vibration at room temperature to obtain a mixed solution;

[0031] Step B. Place the shaken mixed solution in a high-temperature reaction furnace, heat it to 160° C., keep it warm for 8 hours, and then cool it down to room temperature naturally;

[0032] Step C. Dissolve the reacted mixed solution in DMF, use a silica gel chromatography column (200 mesh) to obtain a red fluorescent carbon dot (r-CDs) solution, add excess ethanol, filter, and retain the supernatant, Put it into a vacuum oven at 60°C for drying to obtain the required red fluorescent carbon dots.

[0033] The DMF in step A was replaced with nitrogen dimethylacetamide (DMAC), and the other steps remained unchanged to obtain the desired green fluorescent carbon do...

Embodiment 2

[0036] Embodiment 2. Preparation of fluorescent carbon dots by hydrothermal method

[0037] Step A. Weigh 1 g of citric acid with a mass fraction of 99% and 4 g of urea, add it to 10 mL of nitrogen-nitrogen dimethylformamide (DMF), and perform ultrasonic vibration at room temperature to obtain a mixed solution;

[0038] Step B. Place the shaken mixed solution in a high-temperature reaction furnace, heat it to 200°C, keep it warm for 4 hours, and then cool it down to room temperature naturally;

[0039] Step C. Dissolve the reacted mixed solution in DMF, use a silica gel chromatography column (200 mesh) to obtain a red fluorescent carbon dot (r-CDs) solution, add excess ethanol, filter, and retain the supernatant, Put it into a vacuum oven at 60°C for drying to obtain the required red fluorescent carbon dots.

[0040] The DMF in step A was replaced with nitrogen dimethylacetamide (DMAC), and the other steps remained unchanged to obtain the desired green fluorescent carbon dots...

Embodiment 3

[0042] Example 3. Preparation of fluorescent carbon dots by microwave method

[0043] Step A. Weigh 0.8g of citric acid and 1.6g of urea and dissolve them in deionized water, and carry out ultrasonic vibration at room temperature to disperse evenly to obtain a mixed solution;

[0044] Step B. The mixed solution was placed in a microwave oven, and after microwave heating for 5 minutes, the solution changed from colorless to brown solid;

[0045] Step C. Dissolve the reacted solid in excess ethanol, and after ultrasonic vibration, repeat the centrifugation three times, keep the supernatant, and dry it in a vacuum oven at 60°C to obtain the required fluorescent carbon dots.

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Abstract

The invention belongs to the technical field of application of fluorescent carbon nano materials, and particularly relates to application of a fluorescent material based on fluorescent carbon dots inchemiluminescence. The fluorescent material is a combination of fluorescent carbon dots and a bis(oxalate)-hydrogen peroxide system, replaces traditional fluorescent dye macromolecules to realize high-brightness and long-lifetime chemiluminescence of the bis(oxalate)-hydrogen peroxide system, avoids contact carcinogenicity of the fluorescent dye macromolecules, and has good biocompatibility and environmental safety. The fluorescent carbon dots can be prepared by a hydrothermal method or a microwave method, and the fluorescent material can be applied to chemiluminescence cold light sources, chemiluminescence analysis or chemiluminescence imaging, and has the advantages of low biotoxicity, high detection sensitivity and high imaging sensitivity.

Description

technical field [0001] The invention belongs to the technical field of application of fluorescent carbon nanomaterials, and in particular relates to the application of a fluorescent material based on fluorescent carbon dots in chemiluminescence. Background technique [0002] The double oxalate chemiluminescence system is known to be the most efficient chemiluminescence system except bioluminescence in nature, and its luminescence application has made important research progress in chemiluminescence cold light source, chemical analysis and detection, chemiluminescence bioimaging, etc. Generally speaking, the bisoxalate-hydrogen peroxide chemiluminescent system is an indirect luminescent system, and its luminescence requires a luminescent medium. Currently, the commonly used chemiluminescent molecules in this chemiluminescent system are fluorescent small molecules such as perylene, rhodamine B, rhodamine G, and rubrene. However, such chemiluminescent dyes generally have certa...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00G01N21/6428
Inventor 娄庆沈成龙单崇新
Owner ZHENGZHOU UNIV
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