Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Domestication screening method of aerobic denitrifying phosphorus accumulating bacteria for processing sewage with low C/N ratio

The technology of denitrifying phosphorus accumulating bacteria and screening method is applied in the field of domestication and screening of aerobic denitrifying phosphorus accumulating bacteria, which can solve the problems of high carbon source dependence, large sludge output, low phosphorus removal efficiency, etc., and achieves a short domestication period. , easy operation, great economic benefits and environmental benefits

Pending Publication Date: 2019-11-22
HUAZHONG UNIV OF SCI & TECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above defects or improvement needs of the prior art, the present invention provides a domestication and screening method of aerobic phosphorus accumulating bacteria for treating low C / N ratio sewage, which is separated, domesticated and screened out from the bottom mud of black and odorous water bodies. An aerobic phosphorus-accumulating bacterium, which has a good dephosphorization and nitrogen removal effect, thereby solving the conflict between nitrifying bacteria, denitrifying bacteria and phosphorus-removing bacteria competing for carbon sources in the traditional nitrogen and phosphorus removal process, and solving The traditional nitrogen and phosphorus removal is highly dependent on carbon sources, the technical problems of low phosphorus removal efficiency and large sludge output

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Domestication screening method of aerobic denitrifying phosphorus accumulating bacteria for processing sewage with low C/N ratio
  • Domestication screening method of aerobic denitrifying phosphorus accumulating bacteria for processing sewage with low C/N ratio
  • Domestication screening method of aerobic denitrifying phosphorus accumulating bacteria for processing sewage with low C/N ratio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Strain selection: The Huxi River in the East Campus of Huazhong University of Science and Technology is a typical black and odorous water body. One liter of sludge was collected from a place 10 cm below the river bottom with a mud picker and stored at -4°C. The total phosphorus in the sediment is 156.3-162.4mg / kg, the total phosphorus in the overlying water is 1.4-1.89mg / L, PO 4 3- -P is 1.13-1.5mg / L, NH 4 + -N is 9.22-22.3mg / L, NO 3 - -N is 2.6-7.4mg / L, NO 2 - -N is 0.01-0.22 mg / L.

[0060] (2) Preparation and inoculation of enrichment medium: Take 100mL of enrichment culture base in a 250mL Erlenmeyer flask, inoculate 1mL of bottom mud and incubate in a 30°C water-bath constant temperature shaker for 72 hours with slight shaking. For the device, see figure 1 , 1 means 250mL Erlenmeyer flask, 2 means water bath constant temperature shaker. The enrichment medium formula is 5g peptone / L, 5g yeast extract extract / L, 10gNaCl / L.

[0061] (3) Primary screening o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of sewage treatment and environmental protection, and more particularly relates to a domestication screening method of aerobic denitrifying phosphorus accumulating bacteria for processing sewage with low C / N ratio. In the invention, black odorous water sediments with a certain nitrogen and phosphorus contents are selected as a source of bacteria, and combined with a suitable culture medium to obtain the aerobic denitrifying phosphorus accumulating bacteria having good nitrogen and phosphorus removal effect on sewage with low C / N ratio through aerobic bacteriaenrichment, aerobic pre-screening and aerobic domestication culture successively. Since the same bacteria has good nitrogen and phosphorus removal effect, the domestication screening method of the invention solves traditional conflict of competition between denitrifying bacteria and dephosphorizing bacteria. The enriched domestication method for the aerobic denitrifying phosphorus accumulating bacteria is simple, easy to operate, short in domestication cycle and significantly higher in screening efficiency.

Description

technical field [0001] The invention belongs to the field of sewage treatment and environmental protection, and more specifically relates to a domestication and screening method for aerobic denitrifying phosphorus-accumulating bacteria for treating low C / N ratio sewage. Background technique [0002] As the eutrophication of water bodies becomes more and more serious, the state has introduced or supplemented sewage discharge standards, especially stricter discharge standards for nitrogen and phosphorus. The traditional biological phosphorus and nitrogen removal treatment is basically based on the combination of biological nitrification, denitrification and biological phosphorus removal mechanism. Denitrification and denitrification are two different biochemical mechanisms, and there are fundamental contradictions, such as the competition of carbon sources, the concentration of dissolved oxygen, and the residence time of sludge. With the downward trend of the C / N and C / P rati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C12R1/01
CPCC12N1/20C12N1/36
Inventor 胡智泉吴丁山刘冬啟肖波刘石明包美玲周志勤
Owner HUAZHONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com