33results about How to "Short domestication period" patented technology

In the treatment of waste water containing binary acid, biological fluidized bed technology and Ag / TiO2 photocatalytic oxidation technology are adopted. Stuffing is added into the biological fluidized bed to increase the microbe living space, increase biomass and raise organic load rate; and partial water is made to circulate to increase oxygen dissolved in waste water and eliminate organic matters in water effectively in constant aeration condition. The effluent from the biological fluidized bed is then treated in Ag / TiO2 photocatalytic oxidation equipment with certain amount of catalyst and air as oxidant; and under irradiation of ultraviolet ray the pollutant in water is photocatalytically oxidized. The present invention is suitable for treating waste water containing short-chain and long-chain binary acid, and has obvious treating effects including CODcr eliminating rate of 96.1% and chroma eliminating rate of 97.4%.

A process for culturing the microbes used to biochemically treat high-temp sewage includes such steps as choosing the active sludge in ordinary biochemical aeratnig pool as bacterial seeds, depositing, aerating, adding the sewage of oil field, removing supernatant, adding stabilizer of protein and growth promoter of microbes, and naturolizing.

The invention discloses a high-yield method for cultivating edible fungus morel ear bag materials in standing bags, comprising the following steps: cultivation facilities: an arched plastic greenhouse, the arched plastic greenhouse is a single shed with an area of ​​no more than 2 mu, and the two sides of the shed are from An insect-proof vent of 30 cm is set at a height of 40 centimeters above the ground, and straw curtains are arranged at the vent. , large and thick flesh, pearly white in color, neat fruiting, at least 4 stubbles can be produced, the bacteria are highly resistant to high temperature and disease resistance, vertically cultivated in bags, and are light aerobic edible fungi. It can prevent the root pimples from being too large, and prevent the edges of the expanded leaves from drying out and dying. The advantages of using this technology are high purity, fast germination, short bacterial age, and strong activity.

The invention discloses a taming technique of tissue cultivating sprout water-dielectric, which comprises the following steps: loading tissue cultivating sprout container in the taming chamber; adding fungal inhibiting agent and bacterium inhibiting agent; controlling light, temperature, air moisture to cultivate; placing tissue cultivating sprout in the transparent container with sponge or rock wool; adding taming base; controlling temperature and air moisture.

The invention discloses a tissue-culture rapid propagation and seedling raising method for ficus altissima cv. yellow gem. The tissue-culture rapid propagation and seedling raising method is implemented by five steps of explant culture, explant induction, domestication and subculture multiplication of asepsis material, seedling-strengthening and rooting culture and greenhouse planting. The tissue-culture rapid propagation and seedling raising method is characterized in that a perennial, good and strong ficus altissima cv. yellow gem with excellent maternal characters and no disease and insect damage are selected as a mother plant; and an MS improved medium in all the steps is obtained by changing the used amount of KNO3 in a standard MS medium to be 950mg.L<-1>, changing the used amount of NH4NO3 to be 825mg.L<-1> and removing pure-yellow buds and pure-green buds in time in the culture process. The tissue-culture rapid propagation and seedling raising method disclosed by the invention has the advantages that the survival rate of seedlings is more than 98%, the variation rate is less than or equal to 1.0%, and a great amount of good-quality seedlings can be obtained in a short time.

The invention relates to a suspension cell line and an acclimation method thereof. The acclimation method of the suspension cell line comprises the following steps: resuscitating adherent cells in a culture medium containing 9.5-10.5% of serum, inoculating into a square bottle filled with the culture medium containing 4.5-5.5% of serum, carrying out subculture for 1-3 times, then sequentially transferring the adherent cells from the square bottle, inoculating into shaking flasks with the serum contents in the culture medium of being 3.0-3.6%, 1.8-2.2% and 0.8-1.2% sequentially, carrying out shaking flask suspension culture for 1-4 generations, then transferring into a shaking flask filled with a serum-free culture medium, and carrying out subculture for 1-3 times, so that the suspension cell line is obtained. Compared with the traditional acclimation method, the suspension cell line acclimation method provided by the invention has the advantages that suspension acclimation culture is carried out while serum contents are lowered, cell passage number corresponding to each serum gradient can be maintained to be 1-3 times, acclimation of the adherent cells is controlled to be completed within one month on the whole, the acclimation period is shortened, and acclimation cost is saved. The suspension cell line obtained by adopting the suspension cell line acclimation method has good dispersibility, high viability and high growth rate.

The invention discloses a large-scale vegetative propagation production method of an epipremnum pinnata seedling. The method is achieved by five steps: cultivation of a mother plant, pretreatment and explant collection, sterile material obtaining, domestication and subculture propagation of the sterile material, strong seedling root cultivation, and greenhouse planting. The perennial epipremnum pinnata which is excellent, healthy, and free of plant diseases and insect pests and has female characters is taken as a breeder's seed; the mother plant is cultivated by a cutting seedling obtained from the breeder's seed in a planting manner; a culture medium subjected to inducing, propagation expanding, cultivation and rooting is optimized and improved; rough buds of which the bud roughness is greater than 2.5mm are cut according to 2-4 buds in a cluster when transgenerational clumps are cut; small buds of which the bud roughness is smaller than or equal to 2.5mm are cut by the clumps of 8-10mm; the culture conditions of tissue culture seedlings at each stage are optimized; stable growth in cultivation can be ensured. By adopting the method disclosed by the invention, a great quantity of high-quality nursery-grown plants can be obtained within a short period of time.