Tissue-culture rapid propagation and seedling raising method for ficus altissima cv. yellow gem
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A technology for rapid propagation of seedlings and Fugui banyan group, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems affecting the industrial production of seedlings, easy browning and death of explants, and low multiplication coefficient , to achieve the effect of shortening the domestication cycle, ensuring stable growth, and increasing the multiplication coefficient
Active Publication Date: 2015-04-01
SUNSHINE HORTICULTURE
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[0004] In the existing ficus ficus tissue culture, there are core technical phenomena such as low bacterium acquisition rate, easy browning and death of explants, low multiplication coefficient, and high tissue culture variation, which affect the industrial production of seedlings.
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[0046] The method for fast-propagating seedlings of Ficus fugui tissue culture of the present invention is realized through the following steps:
[0047] 1. Explant cultivation
[0048] Choose the perennial, excellent, robust, and pest-free Ficus Fugui as the mother plant, and spray 20-200PPMM of GA3 on the leaves according to the conventional dosage when the new shoots germinate from April to August in the peak growth season, once every other day After a total of 3 times, the top buds were removed to prevent water from entering, and the light intensity was controlled at 5-10 μmol m -2 ·s -1 , stop fertilizing the mother plant; about 10-20 days later, small buds will grow from the branches, and when the new buds grow to 2-5cm and the bud leaves have yellow-green spots, you can cut off 1-2cm as explants;
[0049] 2. Induction of explants
[0050] Drain the collected explant juice and put it in a sterile container. Use medical cotton with a mass percentage of 0.01-0.03% deter...
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Abstract
The invention discloses a tissue-culture rapid propagation and seedling raising method for ficus altissima cv. yellow gem. The tissue-culture rapid propagation and seedling raising method is implemented by five steps of explant culture, explant induction, domestication and subculture multiplication of asepsis material, seedling-strengthening and rooting culture and greenhouse planting. The tissue-culture rapid propagation and seedling raising method is characterized in that a perennial, good and strong ficus altissima cv. yellow gem with excellent maternal characters and no disease and insect damage are selected as a mother plant; and an MS improved medium in all the steps is obtained by changing the used amount of KNO3 in a standard MS medium to be 950mg.L<-1>, changing the used amount of NH4NO3 to be 825mg.L<-1> and removing pure-yellow buds and pure-green buds in time in the culture process. The tissue-culture rapid propagation and seedling raising method disclosed by the invention has the advantages that the survival rate of seedlings is more than 98%, the variation rate is less than or equal to 1.0%, and a great amount of good-quality seedlings can be obtained in a short time.
Description
technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a method for rapidly growing seedlings by tissue culture of Ficus altissima cv. 'Yellow Gem'. technical background [0002] Fugui banyan, also known as golden leaf banyan, horse banyan, chicken banyan, big green tree, big leaf banyan (Hainan Island), belongs to the genus Moraceae, and is a perennial everwoody foliage plant. It is native to Hainan, Guangxi, Yunnan (south to central, northwest), southern Jiangxi, Sichuan and India (Andaman Islands), Myanmar, Vietnam, Thailand, Malaysia, Indonesia, Philippines and other countries. Its nature likes high temperature and humid climate, is resistant to barrenness and drought, and has strong wind and pollution resistance. Rapid growth, easy to survive transplanting. [0003] Ficus ficus has scattered yellow patches on its leaves and is suitable for use as a street tree, landscape tree, and shade tree. It is also a...
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