A rhizoma bletillae seedling growth promoting method based on piriformospora indica

Abstract

The invention discloses a rhizoma bletillae seedling growth promoting method based on piriformospora indica, which comprises the following steps: sterilizing a substrate for transplanting and domesticating rhizoma bletillae seedlings to obtain a sterilized seedling substrate; preparing a piriformospora indica bacterial solution with the concentration of 25-50g. L <-1 >, or preparing a piriformospora indica spore solution with the concentration of 1-3 * 10 < 8 > cfu / mL; using the piriformospora indica bacterial liquid or piriformospora indica spore liquid for the rhizoma bletillae seedlings discharged from the bottle in a soaking or irrigating mode; and transplanting rhizoma bletillae to the substrate, and planting until outplanting. The method can shorten the domestication period of the rhizoma bletillae tissue culture seedlings, and the rhizoma bletillae seedlings can be outplanted in advance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a growth-promoting method for Pyromorpha indica seedlings. Background technique [0002] Baiji (Bletilla striata (Thunb.) Reichb.f.), also known as Lianjicao, Gangen, Baiji, is a perennial herbaceous plant in the family Orchidaceae. The purple or pink flowers are not only of high ornamental value, but also It is one of the traditional Chinese medicines in my country and is included in the Pharmacopoeia of the People's Republic of China. It is mainly used as a medicine with dried tubers, which can be harvested after planting for 4 to 5 years. [0003] White and dry tubers mainly contain bibenzyls, phenanthrenes and their derivatives, which have the effects of astringent and hemostasis, detumescence and muscle growth, and are used in clinical medicine to treat wound bleeding, burns and sores externally, and internally Hematemesis, lung disease, hemoptysis, chronic gastric u...

AI Technical Summary

Problems solved by technology

Although only mycorrhizal fungi culture, co-cultivation with Bletilla seeds, and artificial seed embedding in this patent require aseptic operations, the complicated pretreatment procedures for Bletilla seeds increase the risk of seed contamination, and Bletilla seeds are mixed with Bletilla seeds. The liquid culture of mycorrhizal fungi is more likely to be polluted than the solid culture. The upper part of the substrate of the artificial seed seedlings described in this patent is sprayed and poured with a mixed solution of coconut milk, banana juice and tomato juice with a volume ratio of 1:3:5. , is a rich source of nutrition for the miscellaneous bacteria in the seedling medium, which will cause a large number of miscellaneous bacteria to grow in the matrix, causing the artificial seeds to rot or affect the germination and growth of the seeds. In addition, only the artificial seeds are sown in the seedling pots and transplanted to the field after 3 months. Survival rate, but does not specify the specifications of the seedlings transplanted at this time, the size of the seedlings affects the growth potential and the cycle of the medicine

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