Production method of composite microbial agent for treatment of refractory wastewater

A composite microbial inoculum and a technology for composite microbial bacteria are applied in the field of production of composite microbial inoculants for refractory wastewater treatment, and achieve the effects of low production cost, good cultivation effect and low price.

Inactive Publication Date: 2016-04-27
BLUESTAR LEHIGH ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a new production method for the production of complex microbial inoculants for refractory wastewater treatment in view of the defects in the application technology of the existing compound microbial inoculum for refractory wastewater treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, a kind of production method of refractory waste water treatment composite microbial bacterial agent, comprises the following steps:

[0025] (1) Medium preparation: add corn steep liquor, molasses (weight ratio 2:1) or other extractive organic carbon sources to the basic medium, so that the TOC concentration of the medium is 500mg / L;

[0026] (2) Bacterial flora inoculation: add complex microbial flora to the medium, and the amount added is 1% of the target culture concentration; and the source of the complex microbial flora used includes but is not limited to commercially available complex microbial flora, soil, sludge, Pollution of river sediment;

[0027] (3) Bacteria culture: control dissolved oxygen 0.2mg / L, microbial load 0.1kgTOC / (kgMLSS.d), carry out mixed culture of the inserted complex microbial flora, and ensure the TOC removal of the medium by the complex microbial flora The rate is not less than 80%;

[0028] (4) Scale-up culture: Carry out ...

Embodiment 2

[0029] Embodiment 2, a kind of production method of refractory waste water treatment composite microbial bacterial agent, comprises the following steps:

[0030] (1) Medium preparation: Add corn steep liquor, molasses (weight ratio 1:2) or other extractive organic carbon sources to the basic medium, so that the TOC concentration of the medium is 5000 mg / L;

[0031] (2) Bacterial flora inoculation: add complex microbial flora to the medium, and the amount added is 10% of the target culture concentration; and the source of the complex microbial flora used includes but is not limited to commercially available complex microbial flora, soil, sludge, Pollution of river sediment;

[0032] (3) Bacteria culture: control dissolved oxygen 2.0mg / L, microbial load 0.5kgTOC / (kgMLSS.d), carry out mixed culture of the inserted complex microbial flora, and ensure that the complex microbial flora removes TOC from the medium The rate is not less than 80%;

[0033] (4) Scale-up culture: Carry o...

Embodiment 3

[0034] Embodiment 3, a kind of production method of refractory wastewater treatment composite microbial bacterial agent, comprises the following steps:

[0035] (1) Medium preparation: Add corn steep liquor, molasses or other extractive organic carbon sources to the basal medium so that the TOC concentration of the medium is 3000 mg / L;

[0036] (2) Bacterial flora inoculation: add complex microbial flora to the medium, and the amount added is 5% of the target culture concentration; and the source of the complex microbial flora used includes but is not limited to commercially available complex microbial flora, soil, sludge, Pollution of river sediment;

[0037] (3) Bacteria culture: control dissolved oxygen 1.0mg / L, microbial load 0.3kgTOC / (kgMLSS.d), carry out mixed culture of the inserted complex microbial flora, and ensure that the complex microbial flora removes TOC from the medium The rate is not less than 80%;

[0038] (4) Scale-up culture: Carry out step-by-step scale-...

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Abstract

The invention relates to a production method of a composite microbial agent for treatment of refractory wastewater. The steps of the production method include: adding corn pulp, molasses or other composite carbon source into a production medium, inoculating a composite microbial community, and carrying out stepwise amplification culture under the conditions of dissolved oxygen 0.2-2.0 mg / L, microbial load 0.1-0.5 kgTOC / (kgSS. d) and TOC removal rate > / =80%. By using the production method, it is possible to produce the composite microbial agent in batch, and it is possible to omit strain compounding work from an agent production process. The production method has simple steps and good effect; the preparation cycle of the composite microbial agent is shortened, production cost is lowered, and the produced composite microbial agent is particularly suitable for the treatment of refractory wastewater.

Description

technical field [0001] The invention belongs to the field of environment-friendly microorganisms, and in particular relates to a production method of a composite microbial bacterial agent for refractory wastewater treatment. Background technique [0002] The use of special microorganisms to treat wastewater, especially industrial wastewater, has a good effect. The effect of composite microbial agents on wastewater treatment is usually better than that of single microbial agents on wastewater treatment. The reason is not only that composite microbial agents have more microbial flora, but also because the ability of composite microbial agents to adapt to wastewater quality is better than that of single microbial agents. Single strain bacterial agent. [0003] The production of a single microbial agent usually goes through multiple steps such as medium preparation, sterilization, inoculation, fermentation culture, quality inspection, and packaging. The production of compound ...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/00C02F3/34
CPCC02F3/34C12N1/00C12N1/36
Inventor 徐军李坤王开春何一群赵焰崔庆兰张彬彬田凤蓉
Owner BLUESTAR LEHIGH ENG INST CO LTD
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