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Large-scale vegetative propagation production method of epipremnum pinnata seedling

A technology of vegetative propagation and production method, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems such as inability to obtain a large number of high-quality seedlings, reduce tissue culture browning, improve the acquisition rate, and satisfy the survival rate. Effect

Active Publication Date: 2014-04-30
SUNSHINE HORTICULTURE
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AI Technical Summary

Problems solved by technology

[0003] The traditional propagation of Kylin tail is to adopt the method of cutting seedlings, which cannot obtain a large number of high-quality seedlings in a short period of time

Method used

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Embodiment Construction

[0049] In the present invention, the MS improved medium used is to add 220mg.L in the conventional MS medium -1 Ca(NO 3 ) 2 .4H 2 O, and KNO 3 Dosage from 1900mg.L -1 increased to 2100mg.L -1 .

[0050] In the present invention, the peat soil used is provided by Danish Pinshi Company, adopts high-quality peat moss, and the organic matter content reaches 98%.

[0051] The large-scale asexual reproduction production method of unicorn tail seedlings of the present invention is realized by the following steps:

[0052] 1. Cultivation, pretreatment and explant collection of mother plants

[0053] Due to the high maturity of the branches of the adult mother plant and the high content of phenolic substances, if it is used as an explant, it will seriously affect the acquisition rate of the sterile body and prolong the induction and domestication period. Therefore, the present invention selects excellent, robust, no damage by diseases and insect pests, and the perennial unicorn...

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Abstract

The invention discloses a large-scale vegetative propagation production method of an epipremnum pinnata seedling. The method is achieved by five steps: cultivation of a mother plant, pretreatment and explant collection, sterile material obtaining, domestication and subculture propagation of the sterile material, strong seedling root cultivation, and greenhouse planting. The perennial epipremnum pinnata which is excellent, healthy, and free of plant diseases and insect pests and has female characters is taken as a breeder's seed; the mother plant is cultivated by a cutting seedling obtained from the breeder's seed in a planting manner; a culture medium subjected to inducing, propagation expanding, cultivation and rooting is optimized and improved; rough buds of which the bud roughness is greater than 2.5mm are cut according to 2-4 buds in a cluster when transgenerational clumps are cut; small buds of which the bud roughness is smaller than or equal to 2.5mm are cut by the clumps of 8-10mm; the culture conditions of tissue culture seedlings at each stage are optimized; stable growth in cultivation can be ensured. By adopting the method disclosed by the invention, a great quantity of high-quality nursery-grown plants can be obtained within a short period of time.

Description

technical field [0001] The invention relates to the technical field of plant asexual reproduction, in particular to a method for large-scale production of unicorn tail [Epipremnum pinniatum] seedlings. technical background [0002] Kylintail, also known as Kylinleaf, Tree Dragon, and Flying Centipede, belongs to the genus Kylintail in the family Araceae, and is a perennial evergreen vine foliage plant. It is native to Guangdong, Guangxi, Hainan, Taiwan provinces and regions in southern China, as well as India, Malaysia and other countries. Its nature likes a warm, humid and shaded environment. Stems and leaves can be used for medicine, clearing heat and cooling blood, promoting blood circulation, removing blood stasis, detoxifying and reducing swelling; mainly treating cold and fever; epistaxis; red eyes and swelling pain; whooping cough; bruises; bone fractures; rheumatic arthralgia; Boil; snakebite. Kylin's caudal leaves are dark green and lustrous. The young leaves hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李雪叶清梅简丽观钟春德黄雨花黄惠玲杨双霞
Owner SUNSHINE HORTICULTURE
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